LOCUS       sequence-25827-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Chang MC, Eachus RA, Trieu W, Ro DK, Keasling JD
  TITLE     Engineering Escherichia coli for production of functionalized 
            terpenoids using plant P450s.
  JOURNAL   Nat Chem Biol. 2007 May . 3(5):274-7.
  PUBMED    17438551
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2007 May . 3(5):274-7."
COMMENT     Sequence Label: pDUET-aaCPRct-A13AMO
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     68..87
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        68..86
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    87..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             126..148
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc
       61 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
      121 ataattttgt ttaactttaa gaaggagata taccatggcg ctggataaac tggatctgta
      181 tgtgatcatt acgctggtag ttgcggttgc tgcttatttc gcgaagaacc gccgtagcag
      241 ctctgcggcc aagaaagcag ctgaatctcc tgttatcgtc gtaccaaaaa aagtcaccga
      301 ggacgaagta gacgatggtc gtaaaaaggt taccgtgttc ttcggcaccc aaactggcac
      361 tgcagaaggc ttcgcgaaag cgctggtcga agaagctaag gctcgttatg aaaaagcggt
      421 tttcaaggta atcgatctgg atgactacgc tgcggaagat gacgaatatg aagaaaaact
      481 gaagaaagag tctctggcct tcttctttct ggctacgtac ggtgatggcg aaccgaccga
      541 caacgcagcg cgcttctata aatggtttac tgaaggtgaa gaaaaaggcg agtggctgga
      601 taagctgcag tacgctgtgt tcggcctggg taaccgtcag tacgaacact tcaacaagat
      661 cgctaaagtt gtcgatgaaa aactggtcga acaaggcgct aaacgcctgg tcccnnntgg
      721 catgggtnnn annnnngncc anatgcncga nannntcccc tcaccgccat ggaaagaagc
      781 tgggtttggg ccgggactgt gatnnnntgn gtggcgggnn aa
//
