LOCUS       sequence-258286-        9187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express both AsCas12a and CRISPR arrays on single transcripts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Campa CC, Weisbach NR, Santinha AJ, Incarnato D, Platt RJ
  TITLE     Multiplexed genome engineering by Cas12a and CRISPR arrays encoded 
            on single transcripts.
  JOURNAL   Nat Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6.
  PUBMED    31406383
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Aug 12. pii: 10.1038/s41592-019-0508-6. doi: 
            10.1038/s41592-019-0508-6."
COMMENT     Sequence Label: pCE048-SiT-Cas12a
FEATURES             Location/Qualifiers
     source          1..9187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        233..1411
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          464..1402
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1359..1379
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1446..5366
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5086..5095
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5367..5414
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5421..5501
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     5698..5714
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5698..5714
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    5775..6363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5828..5848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6246..6257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     repeat_region   6450..6590
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6450..6579
                     /label=AAV2 ITR
     rep_origin      6665..7120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6752..6771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6962..6983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7137..7156)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7256..7278
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7316..7334)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7402..7506
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7507..7575,7576..8367)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7725..7744)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8538..9126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9027..9046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaatt aatgcaaaga tggataaagt tttaaacaga
      181 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
      241 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
      301 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
      361 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
      421 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
      481 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
      541 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
      601 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
      661 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
      721 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
      781 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
      841 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
      901 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
      961 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     1021 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     1081 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     1141 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     1201 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     1261 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     1321 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     1381 aagttttttt cttccatttc aggtgtcgtg agctagcgtt taaacttaag cttggtaccg
     1441 ccaccatgac acagttcgag ggctttacca acctgtatca ggtgagcaag acactgcggt
     1501 ttgagctgat cccacagggc aagaccctga agcacatcca ggagcagggc ttcatcgagg
     1561 aggacaaggc ccgcaatgat cactacaagg agctgaagcc catcatcgat cggatctaca
     1621 agacctatgc cgaccagtgc ctgcagctgg tgcagctgga ttgggagaac ctgagcgccg
     1681 ccatcgactc ctatagaaag gagaaaaccg aggagacaag gaacgccctg atcgaggagc
     1741 aggccacata tcgcaatgcc atccacgact acttcatcgg ccggacagac aacctgaccg
     1801 atgccatcaa taagagacac gccgagatct acaagggcct gttcaaggcc gagctgttta
     1861 atggcaaggt gctgaagcag ctgggcaccg tgaccacaac cgagcacgag aacgccctgc
     1921 tgcggagctt cgacaagttt acaacctact tctccggctt ttatgagaac aggaagaacg
     1981 tgttcagcgc cgaggatatc agcacagcca tcccacaccg catcgtgcag gacaacttcc
     2041 ccaagtttaa ggagaattgt cacatcttca cacgcctgat caccgccgtg cccagcctgc
     2101 gggagcactt tgagaacgtg aagaaggcca tcggcatctt cgtgagcacc tccatcgagg
     2161 aggtgttttc cttccctttt tataaccagc tgctgacaca gacccagatc gacctgtata
     2221 accagctgct gggaggaatc tctcgggagg caggcaccga gaagatcaag ggcctgaacg
     2281 aggtgctgaa tctggccatc cagaagaatg atgagacagc ccacatcatc gcctccctgc
     2341 cacacagatt catccccctg tttaagcaga tcctgtccga taggaacacc ctgtctttca
     2401 tcctggagga gtttaagagc gacgaggaag tgatccagtc cttctgcaag tacaagacac
     2461 tgctgagaaa cgagaacgtg ctggagacag ccgaggccct gtttaacgag ctgaacagca
     2521 tcgacctgac acacatcttc atcagccaca agaagctgga gacaatcagc agcgccctgt
     2581 gcgaccactg ggatacactg aggaatgccc tgtatgagcg gagaatctcc gagctgacag
     2641 gcaagatcac caagtctgcc aaggagaagg tgcagcgcag cctgaagcac gaggatatca
     2701 acctgcagga gatcatctct gccgcaggca aggagctgag cgaggccttc aagcagaaaa
     2761 ccagcgagat cctgtcccac gcacacgccg ccctggatca gccactgcct acaaccctga
     2821 agaagcagga ggagaaggag atcctgaagt ctcagctgga cagcctgctg ggcctgtacc
     2881 acctgctgga ctggtttgcc gtggatgagt ccaacgaggt ggaccccgag ttctctgccc
     2941 ggctgaccgg catcaagctg gagatggagc cttctctgag cttctacaac aaggccagaa
     3001 attatgccac caagaagccc tactccgtgg agaagttcaa gctgaacttt cagatgccta
     3061 cactggcctc tggctgggac gtgaataagg agaagaacaa tggcgccatc ctgtttgtga
     3121 agaacggcct gtactatctg ggcatcatgc caaagcagaa gggcaggtat aaggccctga
     3181 gcttcgagcc cacagagaaa accagcgagg gctttgataa gatgtactat gactacttcc
     3241 ctgatgccgc caagatgatc ccaaagtgca gcacccagct gaaggccgtg acagcccact
     3301 ttcagaccca cacaaccccc atcctgctgt ccaacaattt catcgagcct ctggagatca
     3361 caaaggagat ctacgacctg aacaatcctg agaaggagcc aaagaagttt cagacagcct
     3421 acgccaagaa aaccggcgac cagaagggct acagagaggc cctgtgcaag tggatcgact
     3481 tcacaaggga ttttctgtcc aagtatacca agacaacctc tatcgatctg tctagcctgc
     3541 ggccatcctc tcagtataag gacctgggcg agtactatgc cgagctgaat cccctgctgt
     3601 accacatcag cttccagaga atcgccgaga aggagatcat ggatgccgtg gagacaggca
     3661 agctgtacct gttccagatc tataacaagg actttgccaa gggccaccac ggcaagccta
     3721 atctgcacac actgtattgg accggcctgt tttctccaga gaacctggcc aagacaagca
     3781 tcaagctgaa tggccaggcc gagctgttct accgccctaa gtccaggatg aagaggatgg
     3841 cacaccggct gggagagaag atgctgaaca agaagctgaa ggatcagaaa accccaatcc
     3901 ccgacaccct gtaccaggag ctgtacgact atgtgaatca cagactgtcc cacgacctgt
     3961 ctgatgaggc cagggccctg ctgcccaacg tgatcaccaa ggaggtgtct cacgagatca
     4021 tcaaggatag gcgctttacc agcgacaagt tctttttcca cgtgcctatc acactgaact
     4081 atcaggccgc caattcccca tctaagttca accagagggt gaatgcctac ctgaaggagc
     4141 accccgagac acctatcatc ggcatcgatc ggggcgagag aaacctgatc tatatcacag
     4201 tgatcgactc caccggcaag atcctggagc agcggagcct gaacaccatc cagcagtttg
     4261 attaccagaa gaagctggac aacagggaga aggagagggt ggcagcaagg caggcctggt
     4321 ctgtggtggg cacaatcaag gatctgaagc agggctatct gagccaggtc atccacgaga
     4381 tcgtggacct gatgatccac taccaggccg tggtggtgct ggagaacctg aatttcggct
     4441 ttaagagcaa gaggaccggc atcgccgaga aggccgtgta ccagcagttc gagaagatgc
     4501 tgatcgataa gctgaattgc ctggtgctga aggactatcc agcagagaaa gtgggaggcg
     4561 tgctgaaccc ataccagctg acagaccagt tcacctcctt tgccaagatg ggcacccagt
     4621 ctggcttcct gttttacgtg cctgccccat atacatctaa gatcgatccc ctgaccggct
     4681 tcgtggaccc cttcgtgtgg aaaaccatca agaatcacga gagccgcaag cacttcctgg
     4741 agggcttcga ctttctgcac tacgacgtga aaaccggcga cttcatcctg cactttaaga
     4801 tgaacagaaa tctgtccttc cagaggggcc tgcccggctt tatgcctgca tgggatatcg
     4861 tgttcgagaa gaacgagaca cagtttgacg ccaagggcac ccctttcatc gccggcaaga
     4921 gaatcgtgcc agtgatcgag aatcacagat tcaccggcag ataccgggac ctgtatcctg
     4981 ccaacgagct gatcgccctg ctggaggaga agggcatcgt gttcagggat ggctccaaca
     5041 tcctgccaaa gctgctggag aatgacgatt ctcacgccat cgacaccatg gtggccctga
     5101 tccgcagcgt gctgcagatg cggaactcca atgccgccac aggcgaggac tatatcaaca
     5161 gccccgtgcg cgatctgaat ggcgtgtgct tcgactcccg gtttcagaac ccagagtggc
     5221 ccatggacgc cgatgccaat ggcgcctacc acatcgccct gaagggccag ctgctgctga
     5281 atcacctgaa ggagagcaag gatctgaagc tgcagaacgg catctccaat caggactggc
     5341 tggcctacat ccaggagctg cgcaacaaaa ggccggcggc cacgaaaaag gccggccagg
     5401 caaaaaagaa aaagggatcc tacccatacg atgttccaga ttacgcttat ccctacgacg
     5461 tgcctgatta tgcataccca tatgatgtcc ccgactatgc ctaagaattc gattcgtcag
     5521 tagggttgta aaggtttttc ttttcctgag aaaacaacct tttgttttct caggttttgc
     5581 tttttggcct ttccctagct ttaaaaaaaa aaaagcaaaa ctcaccgagg cagttccata
     5641 ggatggcaag atcctggtat tggtctgcga aatttctact cttgtagatt gaagagccag
     5701 gaaacagcta tgacgctctt ctaatttcta ctcttgtaga ttctagaaaa gaattcgata
     5761 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5821 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     5881 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5941 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6001 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6061 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6121 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     6181 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6241 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6301 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6361 cgcatcgata ccgagcgcta ataaaagatc tttattttca ttagatctgt gtgttggttt
     6421 tttgtgtcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     6481 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     6541 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     6601 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     6661 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6721 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     6781 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6841 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     6901 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6961 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     7021 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     7081 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     7141 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     7201 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cttctccggg
     7261 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgatacg aaagggcctc
     7321 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7381 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7441 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7501 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7561 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7621 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7681 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7741 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7801 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7861 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7921 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7981 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8041 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     8101 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     8161 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8221 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8281 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8341 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8401 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8461 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8521 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8581 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8641 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8701 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8761 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8821 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8881 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8941 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     9001 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9061 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9121 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9181 cacatgt
//
