LOCUS       sequence-25862-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  TITLE     FQG plasmids
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEF 3-YFP-2
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(115..366)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(157..178)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     506..525
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc
       61 ttataatgcc aactttgtac aagaaagctg ggtagtacct tagctcatga ctgacttgta
      121 gagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      181 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg tagctcaggt agtggttgtc
      241 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      301 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
      361 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
      421 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
      481 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
      541 gcgctcctgg gcgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
      601 gtcggggtag cgggcgaagc acttcaggcc gtagccgaag gtggtcacga gggtgggcca
      661 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaagtggc
      721 atcgccctcg ccctcg
//
