LOCUS       sequence-25875-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR 
            insertion, Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X2 Hygro DEST (w17-1)
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      36..171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    211..332
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(222..241)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     276..295
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             404..584
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 acgactcact atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta
       61 cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag
      121 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
      181 taggggcggg atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca
      241 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      301 ttgtaaccat tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt
      361 gcaagttata aatactgaat aataagatga catgaactac tactgctaga gattttccac
      421 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
      481 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
      541 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc
//
