LOCUS       sequence-25882-e         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for miRNA expression under CMV/TO promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR/pSM2(CMV/TO) (w55-1)
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     ncRNA           81..174
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           188..300
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(332..431)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(543..562)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ctctccctat cagtganaga ganctcccta tcagtgatag agatcgtcga ctagtcgact
       61 agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca
      121 catcttggaa acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctcgagca
      181 accagaattc aaggggctac tttaggagca attatcttgt ttactaaaac tgaatacctt
      241 gctatctctt tgatacattt ttacaaagct gaattaaaat ggtataaatt aaatcacttt
      301 tttcaattgg aagactaatg cgtttatcta gacccagctt tcttgtacaa agttggcatt
      361 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      421 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
      481 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
      541 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
      601 catg
//
