LOCUS       sequence-25887-e         612 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Empty control vector, CMV/TO promoter, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pQCXP CMV/TO empty (w368-1)
FEATURES             Location/Qualifiers
     source          1..612
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    61..85
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(154..178)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    219..237
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    240..258
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(260..463)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(289..309)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aattccggat ccgttaatta agcgtacgag gcctaccggt ggccactgtg ctggatatca
       61 accactttgt acaagaaagc tgggtctaga tatctcgagt gcggccgcga attcngtacc
      121 ggatccagtc gactgaattg gttcccatgg tggagcctgc ttttttgtac aaacttgttg
      181 atatctgcag aattccacca cactggacta gtcgacgatc tctatcactg atagggagat
      241 ctctatcact gatagggaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
      301 ccatttgcgt caatggggcg gagttgttac gacnttttgg aaagtcccgt tgattttggt
      361 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
      421 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atancgatga
      481 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
      541 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
      601 cttgatgtac tg
//
