LOCUS       sequence-25925-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector, Degron N-term Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pL106P-LUC-ENTR1A(w451-1)
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(48..368,369..371))
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     regulatory      368..377
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(408..507)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(526..549)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(557..584)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gatggcgccg ggcctttctt tatgtttttg gcgtcttcca tgaattcttc cggttttaga
       61 agctccacat cgaagacgag agtggcatgt ggtgggatga tgcctgggtg cccagtggca
      121 ccataggcat aatctggaga tatagtcagt ttggctctct gacccacact catctgggca
      181 accccttctt cccagcctcg gatcacctcc tgcttgccta gcataaactt aaagggcttg
      241 tttctgtccc gggaggaatc gactttcttt ccatcttcaa gcatcccggt gtagtgcacc
      301 acacaggtct ggccgcgctt ggggaaggtg cgcccgtctc ctggggagat ggtttccacc
      361 tgcactccca tggtggcgga tccagtcgac tgaattggtt cctttaaagc ctgctttttt
      421 gtacaaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac
      481 tatcagtcaa aataaaatca ttatttgggg cccgagctta agtaactaac taacaggaag
      541 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
      601 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
      661 atccgctccc ggcggatttg tcctactcag gaaagcgcac ccgac
//
