LOCUS       sequence-25927-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector for EGFPshRNA#2 under H1/TO promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pENTR/pTER shEGFP #2 (728-2)
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..43
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(175..274)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(386..405)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(464..483)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctttggattt gggatcttat aagttcccta tcagtgatag agatccccgc tggagtacaa
       61 ctacaacttc aagagagttg tagttgtact ccagcttttt ggaaaagctt ggtaccgagc
      121 tcggatccac tagtccagtg tggtggaatt cgcggccgca ctcgagatat ctagacccag
      181 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
      241 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg
      301 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
      361 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
      421 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
      481 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
      541 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
      601 agactaaact ggctgacgga att
//
