LOCUS       sequence-259304-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid includes a EGFP gene driven by a minimal CMV promoter. 
            Cis-regulatory elements can be cloned into the single EcoRV site. 
            This plasmid can be used for generating a lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Roman Azcona MS, Fang Y, Carusillo A, Cathomen T, Mussolino C
  TITLE     A versatile reporter system for multiplexed screening of effective 
            epigenome editors.
  JOURNAL   Nat Protoc. 2020 Oct;15(10):3410-3440. doi: 
            10.1038/s41596-020-0380-y. Epub 2020 Sep 4.
  PUBMED    32887975
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41596-020-0380-y"; journalName: "Nat Protoc"; date: 
            "2020-10"; volume: "15"; issue: "10"; pages: "3410-3440"
COMMENT     Sequence Label: pCCl-MCS_miniCMV_EGFP
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(15..17,18..20,21..734)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(15..17,18..20,21..731)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(60..81)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(321..340)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     668..689
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgcggaatt caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
       61 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      121 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      181 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      241 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      301 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      361 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      421 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
      481 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
      541 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      601 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      661 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      721 agctgtacaa gtaaagcg
//
