LOCUS       sequence-259353-        8008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of KIF1C-mCherry in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Theisen U, Straube E, Straube A
  TITLE     Directional persistence of migrating cells requires Kif1C-mediated 
            stabilization of trailing adhesions.
  JOURNAL   Dev Cell. 2012 Dec 11;23(6):1153-66. doi: 
            10.1016/j.devcel.2012.11.005.
  PUBMED    23237952
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2012.11"; journalName: "Dev Cell"; date: 
            "2012-12-11- 11"; volume: "23"; issue: "6"; pages: "1153-66"
COMMENT     Sequence Label: pKIF1C-mCherry
FEATURES             Location/Qualifiers
     source          1..8008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    133..254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(170..189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     224..243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(261..716)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(398..419)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     610..629
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        743..847
                     /gene="bla"
                     /label=AmpR promoter
     promoter        849..1206
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        849..1040
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(872..892)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1057..1192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1241..2035
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1241..2035
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1295..1314)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1905..1924
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2223..2242)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2267..2314
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2643..3231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3132..3151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3406..3709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3710..3913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3864..3884
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      6210..6219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7302..7311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7308..8008,1..10)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7453..7471)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7721..7740
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
       61 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      121 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      241 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
      301 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
      361 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
      421 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
      481 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
      541 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
      601 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
      661 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
      721 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      781 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      841 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
      901 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      961 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     1021 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     1081 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     1141 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     1201 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     1261 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     1321 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     1381 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     1441 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     1501 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     1561 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     1621 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     1681 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     1741 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     1801 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     1861 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     1921 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     1981 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     2041 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     2101 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     2161 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     2221 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     2281 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     2341 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     2401 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     2461 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     2521 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2581 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2761 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3301 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta
     3361 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     3421 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3481 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3541 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3601 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3661 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3721 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     3781 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     3841 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     3901 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc
     3961 gagctcaagc ttcgaattct ggagctatgg ctggtgcctc ggtgaaagtg gcagtgaggg
     4021 ttcggccctt taacgcccgt gagaccagcc aggatgccaa gtgtgtggtc agcatgcagg
     4081 gcaacaccac ctccatcatc aatcctaaac agagcaagga tgcccccaaa agcttcacct
     4141 ttgactactc ctactggtca cacacttcga cggaggaccc ccagtttgca tctcagcagc
     4201 aagtgtatcg ggacatcgga gaagagatgc tgctccacgc ctttgaaggc tacaacgtgt
     4261 gcatctttgc ctatgggcag accggggctg ggaaatccta taccatgatg gggcgacagg
     4321 agccagggca gcagggcatc gtgccccagc tctgtgagga cctcttctct cgcgttagtg
     4381 agaaccagag tgctcagcta tcctactctg tggaggtgag ctatatggag atctactgtg
     4441 agcgggtacg agacctcttg aaccccaaga gtcggggttc tctgcgggtc cgggagcacc
     4501 ccatcctggg cccgtacgtg caggacctgt ccaaattggc tgtgacctcc tacgcagaca
     4561 ttgctgacct catggactgt ggaaataaag cacggactgt ggctgccacc aacatgaatg
     4621 agaccagcag ccgttcccat gccgtcttta ccatcgtctt cacacagcgc tgccatgacc
     4681 agctcacggg gctggactcg gagaaggtca gtaagatcag tttggtggac cttgctggga
     4741 gtgagcgagc cgactcctca ggggcccggg gcatgcgcct gaaggaagga gccaacatca
     4801 ataagtccct gactacacta gggaaagtga tctcggccct tgcagatatg caatcaaaga
     4861 agcgaaagtc ggattttatc ccctacaggg actctgtgct cacctggctg ctcaaggaaa
     4921 atttgggggg gaactcacgc acagccatga ttgcagccct gagccctgct gacatcaatt
     4981 acgaggagac tctcagcacc ctcaggtatg ctgaccgcac caagcaaatc cgctgcaatg
     5041 ccatcatcaa cgaggaccct aatgcccggc tgattagaga gctgcaggag gaagtagccc
     5101 ggctgcggga actgctgatg gctcagggac tgtcagcctc tgctctggaa ggcctgaaga
     5161 cggaagaagg gagtgtcaga ggcgccctgc cagctgtgtc atctccccca gctccagttt
     5221 caccctcatc acccaccaca cataatgggg agctggagcc gtcattctcc cccaacacgg
     5281 agtcccagat tgggcctgag gaagccatgg agaggctgca ggagacagag aagattatag
     5341 ctgagctgaa cgagacatgg gaggagaagc tacgcaagac agaagccctg aggatggaga
     5401 gagaagcatt gctggctgag atgggggtgg ccgtccggga ggatggggga actgtgggcg
     5461 tcttctctcc aaagaagact ccccacctgg tgaacctgaa cgaagaccct ctgatgtctg
     5521 agtgtctgct ctaccacatc aaagatggcg tcaccagggt cggccaagta gatatggaca
     5581 tcaagctgac cggacagttc attcgggagc aacactgtct gttccggagc atcccccagc
     5641 cagatggaga agtggtggtc actctggagc cttgtgaagg agctgagaca tatgtgaatg
     5701 ggaagcttgt gacggagccg ctggtgctga agtcagggaa taggattgtg atgggcaaga
     5761 accacgtttt ccgcttcaac cacccggagc aggcaaggct ggaacgggaa cgaggggtcc
     5821 ccccaccccc aggaccgccc tctgagccag tcgactggaa ctttgcccag aaggaactgc
     5881 tggagcagca aggcatcgac ataaagctgg aaatggagaa gaggctgcag gatctggaga
     5941 atcagtaccg gaaagaaaag gaagaagccg atcttctgct ggagcagcag cgactgtatg
     6001 cagactcgga cagcggggat gactctgaca agcgctcttg tgaagagagc tggaggctca
     6061 tctcctcctt gcgggagcag ctgccgccca ccacggtcca gaccattgtc aaacgctgtg
     6121 gtctgcccag cagtggcaag cgcagggccc ctcgcagggt ttatcagatc ccccagcgac
     6181 gcaggctgca gggcaaagac ccccgctggg ccaccatggc tgacctgaag atgcaggcgg
     6241 tgaaggagat ctgctacgag gtggccctgg ctgacttccg ccacgggcgg gctgagattg
     6301 aggccctggc cgccctcaag atgcgggagc tgtgtcgcac ctatggcaag ccagacggcc
     6361 ccggagacgc ctggagggct gtggcccggg atgtctggga cactgtaggc gaggaggaag
     6421 gaggtggagc tggcagtggt ggtggcagtg aggagggagc ccgaggggcg gaggtggagg
     6481 acctccgggc ccacatcgac aagctgacgg ggattctgca ggaggtgaag ctgcagaaca
     6541 gcagcaagga ccgggagctg caggccctgc gggaccgcat gctccgcatg gagagggtca
     6601 tccccctggc ccaggatcat gaggatgaga atgaagaagg tggtgaggtc ccctgggccc
     6661 cgcctgaagg atcagaggca gcagaggagg cagcccccag tgaccgcatg ccgtcagccc
     6721 ggcccccctc gccaccactg tcaagctggg agcgggtgtc acggctcatg gaggaggacc
     6781 ctgccttccg tcgtggtcgt cttcgctggc tcaagcagga gcagctacgg ctgcagggac
     6841 tgcagggctc tgggggccgg ggcggggggc tgcgcaggcc cccagcccgc tttgtgcccc
     6901 ctcacgactg caagctacgc ttccccttca agagcaaccc ccagcaccgg gagtcttggc
     6961 cagggatggg gagcggggag gctccaactc cgctccaacc ccctgaggag gtcactcccc
     7021 atccagccac ccctgcccgc cggcctccga gtccccgaag gtcccaccat ccccgcagga
     7081 actccctgga tggagggggc cgatcccggg gagcgggttc tgcacagcct gaaccccagc
     7141 acttccagcc caaaaagcac aactcttatc cccagccacc ccaaccctac ccagcccagc
     7201 ggcccccagg gccccgctac cccccataca ctactccccc acgaatgaga cggcagcgtt
     7261 ctgcccctga cctcaaggag agtggggcag ctgtggatcc cgccaccatg gtgagcaagg
     7321 gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     7381 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     7441 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     7501 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     7561 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     7621 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     7681 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     7741 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     7801 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     7861 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     7921 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     7981 gccactccac cggcggcatg gacgagct
//
