LOCUS       sequence-25939-e         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral eGFP expression vector, CMV/TO promoter, Puro .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti CMV/TO eGFP Puro (w159-1)
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..69)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    100..688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(153..173)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(571..582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tggacgagct gtacaagtaa agcggccgca ctcgagatat ctagacccag ctttcttgta
       61 caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa
      121 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      181 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      241 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      301 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      361 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
      421 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
      481 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
      541 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
      601 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
      661 cggatctccc tttgggccgc ctccccgcct ggaattctac cgggta
//
