LOCUS       sequence-259398-        6807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Zif268DBD-CRY2PHR under pscADH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6807)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 6807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0320
FEATURES             Location/Qualifiers
     source          1..6807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             19..39
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1848..2035
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1903..1927)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2058..2078)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2058..2076)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2097..2113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2097..2113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2110..2132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2121..2137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2145..2151,2152..2169,2170..2175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2190..2211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2328..2345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2499..3087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2579..2598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3258..4049,4050..4118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3881..3900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4119..4223)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    4260..4763
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     4804..4822
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4860..4882)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4982..5001
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5022..5302
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             5303..5977
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     primer_bind     complement(6217..6238)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6411..6807
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 atctcatata caatggcccc caagaagaag aggaaggttg gtattcacgg gggtacccgc
       61 ccatatgctt gccctgtcga gtcctgcgat cgccgctttt ctcgctcgga tgagcttacc
      121 cgccatatcc gcatccatac cggtcagaag cccttccagt gtcgaatctg catgcgtaac
      181 ttcagtcgta gtgaccacct taccacccac atccgcaccc acacaggcga gaagcctttt
      241 gcctgtgaca tttgtgggag gaagtttgcc aggagtgatg aacgcaagag gcataccaaa
      301 atccatacag gtgctagctt catgaagatg gacaaaaaga ctatagtttg gtttagaaga
      361 gacctaagga ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt
      421 cctgtcttca tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga
      481 tggtggatga aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac
      541 ctcactttaa tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc
      601 ggtgctacaa aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat
      661 accgtaaagg agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta
      721 ttgtatgaac cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct
      781 tactggaaga aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg
      841 ttgatgccaa taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg
      901 ctggagaatg aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga
      961 tggagcaatg ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca
     1021 aagaacagca agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc
     1081 ggggaaataa gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca
     1141 agagataaga acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta
     1201 agagagtatt ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg
     1261 agtcatcttc ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa
     1321 ggcaggaccg gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg
     1381 atgcataaca gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca
     1441 tggaaatggg gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac
     1501 atccttggct ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg
     1561 gacaatcccg cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg
     1621 cttcccgagc ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta
     1681 accgtactca aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac
     1741 atcgacacag ctcgtgagct actagctaaa gctatttcaa gaacccgtga agcacagatc
     1801 atgatcgagc agcagcccgg gtcgacctgc agccaagcta attccgggcg aatttcttat
     1861 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1921 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     1981 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgg
     2041 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     2101 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2161 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2221 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2281 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2341 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2401 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2461 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2521 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2581 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2641 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2701 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2761 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2821 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2881 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2941 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3001 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3061 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3121 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3181 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3241 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3301 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3361 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3421 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3481 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3541 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3601 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3661 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3721 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3781 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3841 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3901 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3961 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4021 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4081 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtccttttca
     4261 tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa
     4321 tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga
     4381 ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct ctcaggtatt
     4441 aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt
     4501 acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa
     4561 atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc
     4621 ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat acaaaacata
     4681 aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga tacgaggcgc
     4741 gtgtaagtta caggcaagcg atccgtctaa gaaaccatta ttatcatgac attaacctat
     4801 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     4861 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4921 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     4981 gcggcatcag agcagattgt actgagagtg caccatagat cacgacatta ctatatatat
     5041 aatataggaa gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg
     5101 ccagatggca gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa
     5161 tcttgatccg gagcttttct ttttttgccg attaagaatt aattcggtcg aaaaaagaaa
     5221 aggagagggc caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa
     5281 gcacacaaag gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt
     5341 ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc
     5401 tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc
     5461 aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt
     5521 ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg
     5581 cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag agttcctcgg
     5641 tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc
     5701 ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga
     5761 acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc ccgaaagctt
     5821 acattttatg ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc ttagattaaa
     5881 tggcgttatt ggtgttgatg taagcggagg tgtggagaca aatggtgtaa aagactctaa
     5941 caaaatagca aatttcgtca aaaatgctaa gaaataggtt attactgagt agtatttatt
     6001 taagtattgt ttgtgcactt gccgatctat gcggtgtgaa ataccgcaca gatgcgtaag
     6061 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     6121 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     6181 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     6241 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     6301 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     6361 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc caacttcttt
     6421 tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca atgacaaaaa
     6481 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
     6541 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca
     6601 cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga
     6661 aataaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt attaatcttt
     6721 tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg cacaatattt
     6781 caagctatac caagcataca atcaact
//
