LOCUS       sequence-259440-        4996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTEF1-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0622
FEATURES             Location/Qualifiers
     source          1..4996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             243..1736
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             complement(join(2262..3053,3054..3122))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2885..2904
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3123..3227)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3330..3918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3819..3838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4497..4910
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4936..4956
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agtcctgcga tcgccgcttt tctcgctcgg atgagcttac ccgccatatc cgcatccata
       61 ccggtcagaa gcccttccag tgtcgaatct gcatgcgtaa cttcagtcgt agtgaccacc
      121 ttaccaccca catccgcacc cacacaggcg agaagccttt tgcctgtgac atttgtggga
      181 ggaagtttgc caggagtgat gaacgcaaga ggcataccaa aatccataca ggtgctagct
      241 tcatgaagat ggacaaaaag actatagttt ggtttagaag agatctaagg attgaggata
      301 atcctgcatt agcagcagct gctcacgaag gatctgtttt tcctgtcttc atttggtgtc
      361 ctgaagaaga aggacagttt tatcctggaa gagcttcaag atggtggatg aaacaatcac
      421 ttgctcactt atctcaatcc ttgaaggctc ttggatctga cctcacttta atcaaaaccc
      481 acaacacgat ttcagcgatc ttggattgta tccgcgttac cggtgctaca aaagtcgtct
      541 ttaaccacct ctatgatcct gtttcgttag ttcgggacca taccgtaaag gagaagctgg
      601 tggaacgtgg gatctctgtg caaagctaca atggagatct attgtatgaa ccgtgggaga
      661 tatactgcga aaagggcaaa ccttttacga gtttcaattc ttactggaag aaatgcttag
      721 atatgtcgat tgaatccgtt atgcttcctc ctccttggcg gttgatgcca ataactgcag
      781 cggctgaagc gatttgggcg tgttcgattg aagaactagg gctggagaat gaggccgaga
      841 aaccgagcaa tgcgttgtta actagagctt ggagtccagg atggagcaat gctgataagt
      901 tactaaatga gttcatcgag aagcagttga tagattatgc aaagaacagc aagaaagttg
      961 ttgggaattc tacttcacta ctttctccgt atctccattt cggggaaata agcgtcagac
     1021 acgttttcca gtgtgcccgg atgaaacaaa ttatatgggc aagagataag aacagtgaag
     1081 gagaagaaag tgcagatctt tttcttaggg gaatcggttt aagagagtat tctcggtata
     1141 tatgtttcaa cttcccgttt actcacgagc aatcgttgtt gagtcatctt cggtttttcc
     1201 cttgggatgc tgatgttgat aagttcaagg cctggagaca aggcaggacc ggttatccgt
     1261 tggtggatgc cggaatgaga gagctttggg ctaccggatg gatgcataac agaataagag
     1321 tgattgtttc aagctttgct gtgaagtttc ttctccttcc atggaaatgg ggaatgaagt
     1381 atttctggga tacacttttg gatgctgatt tggaatgtga catccttggc tggcagtata
     1441 tctctgggag tatccccgat ggccacgagc ttgatcgctt ggacaatccc gcgttacaag
     1501 gcgccaaata tgacccagaa ggtgagtaca taaggcaatg gcttcccgag cttgcgagat
     1561 tgccaactga atggatccat catccatggg acgctccttt aaccgtactc aaagcttctg
     1621 gtgtggaact cggaacaaac tatgcgaaac ccattgtaga catcgacaca gctcgtgagc
     1681 tactagctaa agctatttca agaacccgtg aagcacagat catgatcgga gcagcagccc
     1741 ggggatccta actcgaggcc aattggtgcg gcaattgata ataacgaaaa tgtcttttaa
     1801 tgatctgggt ataatgagga attttccgaa cgtttttact ttatatatat atatacatgt
     1861 aacatatatt ctatacgcta tagagaaagg aaatttttca attaaaaaaa aaatagagaa
     1921 agagtttcac ttcttgatta tcgctaacac taatggttga agtactgcta ctttaatttt
     1981 atgctggatg tgagacgata aatcgcagcc aagtgagtga atagatgacg caccacggtc
     2041 agacacggcc acatcgtatc tcacaggagc aagcgcgata ggagcactca cacatagtac
     2101 ggtgatccgc tgactccttt gcccaaataa gacgtgagcc actagtgcac tgcagtacag
     2161 cggccgcgat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2221 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2281 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2341 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2401 cgggacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2461 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2521 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2581 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2641 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2701 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2761 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2821 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2881 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2941 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3001 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3061 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3121 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3181 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3241 aaagtgccac ctgtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3301 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3361 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3421 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3481 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3541 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3601 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     3661 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     3721 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     3781 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     3841 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     3901 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3961 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg
     4021 gccgcccctg aattcgcatc tagaacgggg tcatcacggc tcatcatgcg ccaaacaaat
     4081 gtgtgcaata cacgctcgga tgactgcatg atgaccgcac tgactgggga cagcagatcc
     4141 acctaagcct gtgagagaag cagacacccg acagatcaag gcagttacgt ctcaccaaac
     4201 cagatgtcaa cacagctaca acgccttgcc aacagggagt tcttcagaga catggaggct
     4261 caaaacgaaa ttattgacag cctagacatc aatagtcata caacagaaag cgaccaccca
     4321 actttggctg ataatagcgt ataaacaatg catactttgt acgttcaaaa tacaatgcag
     4381 tagatatatt tatgcatatt acatataata catatcacat aggaagcaac aggcgcgttg
     4441 gacttttaat tttcgaggac cgcgaatcct tacatcacac ccaatccccc acaagtgatc
     4501 ccccacacac catagcttca aaatgtttct actccttttt tactcttcca gattttctcg
     4561 gactccgcgc atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct
     4621 ctttcttcct ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagac
     4681 accgcctcgt ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt
     4741 ttcttgaaaa tttttttttt tgattttttt ctctttcgat gacctcccat tgatatttaa
     4801 gttaataaac ggtcatcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac
     4861 tttttttact tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttaattac
     4921 aaaagatcta tggcccccaa gaagaagagg aaggttggta ttcacggggg tacccgccca
     4981 tatgcttgcc ctgtcg
//
