LOCUS       sequence-259773-        5152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for serotonin (mammalian expression, membrane 
            localization tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Unger EK, Keller JP, Altermatt M, Liang R, Matsui A, Dong C, Hon OJ,
            Yao Z, Sun J, Banala S, Flanigan ME, Jaffe DA, Hartanto S, Carlen J,
            Mizuno GO, Borden PM, Shivange AV, Cameron LP, Sinning S, Underhill 
            SM, Olson DE, Amara SG, Temple Lang D, Rudnick G, Marvin JS, Lavis 
            LD, Lester HA, Alvarez VA, Fisher AJ, Prescher JA, Kash TL, 
            Yarov-Yarovoy V, Gradinaru V, Looger LL, Tian L
  TITLE     Directed Evolution of a Selective and Sensitive Serotonin Sensor via
            Machine Learning.
  JOURNAL   Cell. 2020 Dec 12. pii: S0092-8674(20)31612-3. doi: 
            10.1016/j.cell.2020.11.040.
  PUBMED    33333022
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Dec 12. pii: S0092-8674(20)31612-3. doi: 
            10.1016/j.cell.2020.11.040."
COMMENT     Sequence Label: pDisplay-iSeroSnFR
FEATURES             Location/Qualifiers
     source          1..5152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        389..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     637..656
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        637..655
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     736..795
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1248..1269
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2380..2409
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2413..2559
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(2581..2598)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2587..2811
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2943..3531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3023..3042)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3702..4493,4494..4562))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4325..4344
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4563..4667)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4694..5149
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4781..4800)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4991..5012
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagaa ctctctggct
      601 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      661 ccaagcttgg taccgaactc ggatccacta gtaacggccg ccagtgtgct ggaattcggc
      721 ttggggatat ccaccatgga gacagacaca ctcctgctat gggtactgct gctctgggtt
      781 ccaggttcca ctggtgacag atctgcgaac gacaccgtag ttgtgggctc gattaaccat
      841 acagaacaga ttatcgtcgc aaacatgttg gcagagatga ttgaggcgca tacagacctt
      901 aaggtggttc gcaaactgaa ccttggcggg gttaacgtta actttgaagc cattaaacgc
      961 ggaggtgcga ataatggtat tgacatttac ctggagtacg ttgggtatgg tcttgtggat
     1021 attctggggt acccggagcc gaacgtctat atcatcgccg acaagcagaa gaacggcatc
     1081 aaggcgaact tcaagatccg ctacaacgtg gaggacggca gcgtgcagct cgccgaccac
     1141 taccagcaga acacccccat cggcgacggc cctgtgctgc tgcctgacaa ccactacctg
     1201 agcacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1261 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     1321 ggagggagca tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1381 ctggacggcg gcgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     1441 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1501 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1561 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1621 atcagcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
     1681 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1741 gggcacaagc tggagtacaa ctttccgccg cccggtgcta ctgatccaga aggtgcatac
     1801 gaaaccgtga agaaggagta caaacgtaaa tggaatattg tatggctcaa accactggga
     1861 ttcaacgctt cgtatgtgct tgcggttaaa gacgaactgg cgaaacagta taaccttaaa
     1921 accttcagtg acttagcgaa aatctcggat aagctgattc tgggtgcaaa catgatgttt
     1981 ttagaaaatc ccgatggtta cccaggcctg caaaaactgt acaatttcaa attcaagcac
     2041 accaaaagca tggacgctgg tattccgtat accgccattg ataataacga agttcaggta
     2101 attgatgcca ctgccactga tggcttgctg gtgagccaca aattaaaaat tctggaggat
     2161 gataaagcgt tcttcccgcc gtattatgct gcccccatca tccgtcagga tgtcttagat
     2221 aagcatcctg aactgaagga cgtgctgaac aaactcgcga atcaaatttc actggaagaa
     2281 atgcagaaac tgaattacaa gcgtgacggt gagggtcagg acccagcgaa agtagctaag
     2341 gagtttttga aagagaaagg tttaattctg caggtcgacg aacaaaaact catctcagaa
     2401 gaggatctga atgctgtggg ccaggacacg caggaggtca tcgtggtgcc acactccttg
     2461 ccctttaagg tggtggtgat ctcagccatc ctggccctgg tggtgctcac catcatctcc
     2521 cttatcatcc tcatcatgct ttggcagaag aagccacgtt aggcggccgc tcgagatcag
     2581 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2641 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2701 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2761 aggattggga agacaatagc aggcgtgctg gggatgcggt gggctctatg gcttctgagg
     2821 cggaaagaac cagtggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2881 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2941 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3001 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3061 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3121 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     3181 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3241 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     3301 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     3361 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     3421 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     3481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3541 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3601 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3661 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3721 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3781 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3841 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3901 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3961 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4021 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4081 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4141 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4201 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4261 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4321 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4381 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4441 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4501 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4561 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4621 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     4681 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5041 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5101 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cg
//
