LOCUS       sequence-260274-        1279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of GFP (as control) tagged with C-terminal
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1279)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-GFP-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(181..183,184..186,187..897)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(226..247)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(487..506)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     834..855
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(917..941)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             943..984
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gngtggctgc gagtgtgttt tgtgagtttt ttaggcacct tttgaatgta atcatttggg
       61 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
      121 gcttttttgt tagacgcaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      181 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      241 ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac
      301 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      361 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      421 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      481 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      541 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      601 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      661 ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      721 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      781 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      841 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
      901 aagggtgggc gcgccgaccc agctttcttg tacaaagtgg gcggcaagcc catccccaac
      961 cccctgctgg gcctggacag caccgctagc aaagacaata ctgtgcctct gaagctgatc
     1021 gctctcctgg ctatggcgag ttccatagtg gcgacagctg ggagaaaccc tgggcatgtc
     1081 cagggccgct atcacagcac attcagactc tgcgcgactg ggggcggtga cgtgtcacgg
     1141 gcccggaaag gctactctct gcccgagcta tccgctgctg acgctaacga ttctgggaaa
     1201 ggctgacggc gggagcgtgn agtcctggcc ngtggtcgac tccaccnatc agatactgtg
     1261 ctggatcgan tccgcccga
//
