LOCUS       sequence-260281-        1215 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK12 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1215)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-CDK12-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1215
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(157..198)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    200..224
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggncgtccga tttctgcatg cagcatcccc actcttcagc tcgccgattc gatccagcag
       61 gtactgattg gtggagtcga ccacaggcag gactgccacg ctcccgccgt ccagctgtcc
      121 cagaatctgt ttagcgttca gcagcgggat gctagcggtg ctgtccaggc ccagcagggg
      181 gttggggatg ggcttgccgc ccactttgta caagaaagct gggttgccgc tcgagcctga
      241 gtaaggaact cctctccctc ttccccctct tggtgggact cttgtaggcc cccgatagag
      301 ttttccataa gcagaagact gagttgggcc cccccagtga aggcctgctc ctgagctgct
      361 ggccccagtg gttcctggcc ccagctcccc atagttttgc aatttggtct ctgcatgtac
      421 cacagaattg ctgcttccct cagccttcag gaatggttgc ccgaccaccg ggtgtaacgc
      481 taaggggacc ctggcaaaac ggaggtcctg gtcccctgga aactgcactg accctgtgcc
      541 ctggtaactg ctggactccc caaggtggct cagagagcct gagaaggtcc tgttcttcac
      601 cagggtctgg accaaggatt ctgtcaaggc tggaccagag tttcgttcat cagtgtcaat
      661 ggcactgaac cctgtttcag gcccatcagt gtttccataa gtcctgtttg gacggggtcg
      721 ggtggagtac tccattggtc tcaaggcctg gtgctcatgt ggcatgtggc caggaggctc
      781 tgcttcatgc tgggataaaa gttgcagcaa ggcggctgtc acggctgggt tcaactcctg
      841 gggggcgctg gaaagatcgc cttcaccaga ggggtggagg tggcggcggt ggaggtccgg
      901 ggggctcagg ggcctcttct ctgctgcctc ctcctgtgca ttgtgggagt cttcggggcc
      961 cctgtggacc actgtcctgg ccctgttgtt ttcctccaga ctgcctgctg gctctgggtt
     1021 ttcctcagct gactcagaaa ctgccaaaat attcttgcat tgtcagctgg agtgctgagc
     1081 cttcaagggt cattcagttc ctgctgangc agatcactgg ggccaagggt gcttccttcc
     1141 aagactccct ctggggccat gnntctgagt ctggctgcga ggnaatacct tccgtcaagg
     1201 tcannaatgg ataga
//
