LOCUS       sequence-260290-        1227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1227)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PKMYT1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(157..198)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    200..224
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggccggccg gatctgcatg cagcatcccc actcttcagc tcgccgattc gatccagcag
       61 gtactgattg gtggagtcga ccacaggcag gactgccacg ctcccgccgt ccagctgtcc
      121 cagaatctgt ttagcgttca gcagcgggat gctagcggtg ctgtccaggc ccagcagggg
      181 gttggggatg ggcttgccgc ccactttgta caagaaagtt ggggttgggt ctagggtgtc
      241 ctcaaacagg ctgaggaggt tccgaggctc aaaggagggg aaggagcccc gaggaggctc
      301 tgagttgatg tcacttaggt ccagggcatc cctgggtgtg cacctgctcc ggggggtgga
      361 ggtgctcccc acagtccggg ccaggacagc ctcaggggag agtgaaggcc ctaggctgtc
      421 gtcatcccag ttgctggaga ggctgctgtc caggagcaaa ctgcagggtg gtgagccagg
      481 cggggtggct ggcgggccca ggggctgtag ccagctggca gggtgagcca gcccatgcca
      541 gagccagcag agcagggcaa gcagggcctg ccacagggcc caccctcggc tcagggcctc
      601 cgctgccatg caccacagca caccccaggc ccgcggctgc ctcaacacag gcagtgccag
      661 cagggcctcg gccgtggccc gcagcttggg gtctggctcc agcatcatga caaggacaga
      721 acgcagctcg gaagacagac cggcagtgaa ctcagggggc aggtagccct ggcgcagctg
      781 ctgccagccc tccccacccg tggggcagct ccatgttgca tgccacttca ggatggtgag
      841 gcccagactg aacacatccg ctgctgtccc ataggagccc tgcagcagct cgggggccat
      901 gtagcggggg tctccctcct ggacctcacc agcttctgct gtacccagct ccaccagcag
      961 tcccgaagtc actccagctt gcagcggccc cgggggcccc aggaaggtgt tggccaggct
     1021 ttgacttcca agggcaccca aggccctagc cttcgcaaag ggccagggca agcatgcttg
     1081 tcccgcagta agcccagaac ctgggccttc aggtagctgg acctccaggc cttacaagtg
     1141 tgcttagcac gctgggctcg accagactcc gttgcatgga catggtgtcc gcctactcct
     1201 cagggcctag gttctagcgc acgcgtt
//
