LOCUS       sequence-260308-        1106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(155..196)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    198..222
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggcctcttat tctgcatgca gcatccccac tcttcagctc gccgattcga tccagcaggt
       61 actgattggt ggagtcgacc acaggcagga ctgccacgct cccgccgtcc agctgtccca
      121 gaatctgttt agcgttcagc agcgggatgc tagcggtgct gtccaggccc agcagggggt
      181 tggggatggg cttgccgccc actttgtaca agaaagctgg gcccatgagg cctgaacttt
      241 tcgggataat tccacatttg gaaagccgcc ccgtcatcat ttccatacag ttagtggtgt
      301 ctgacaagtg ctgggccgca ggcgggtcgt gtgtgctcag cagctgggcc tggatggtct
      361 ccaccaccct cttgaagcga cggctggggc ctgagagcag ggtgaaggtg acggagtaga
      421 tgccgttctc cttctgcgcc tccccaccct ccgtgtaggt gatatcaacc tggaacttga
      481 ccggcttctg gaacacggct ggcccccccg tggccttgta ctcggcccgg aagctcgttt
      541 gggagatgac gctgtggctg agactgggaa tcgacaggaa ggcgtgcacg atgtcagcct
      601 tgatggagct cagaggtttg tctttgatga ccacgaagat ctgctcctcc ttctccaggc
      661 tgatgaagtt cccaaaccag gacttcttcg ccagctctgg ggacgactct ggtgtcaggt
      721 tggacatctc ctccggcgtc ggaacttgca gtttccggcg gtggaagcgg ggtgagccca
      781 gaaagctgtt cttgatggag ttgagccgcg ccctccaggg cacccctccg acgctggggc
      841 tggacggggc gtggggttgg gcgtgccagc cgggctctcc tttggcgtgt ggacaggtgt
      901 cccttggggt ggggagggga ctgccccttg gtgagggtga ggggtcaccc gggggctgct
      961 gatggctggt ggaaaggcct gagagcaacg ctgatggacg agactctgcc gtgctgggcc
     1021 atctcaatgg ccggcgcgca gcaccgggga gccgcgtctg tcacgctgag cacctcatgg
     1081 attgcgntct gacgccgctt gccggc
//
