LOCUS       sequence-260326-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(155..196)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    198..222
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggtggctat tctgcatgca gcatccccac tcttcagctc gccgattcga tccagcaggt
       61 actgattggt ggagtcgacc acaggcagga ctgccacgct cccgccgtcc agctgtccca
      121 gaatctgttt agcgttcagc agcgggatgc tagcggtgct gtccaggccc agcagggggt
      181 tggggatggg cttgccgccc actttgtaca agaaagctgg gttgggcaga ggggtcccgt
      241 tggtggccag gagcttcttg tccttggggg ggcctcggcg gggagagctg ctcagctctg
      301 ggtctgggcg gccgggtggg ggctgcaggc ttcggggtgg ggcaccagca ggccgggtct
      361 gggccccgtt cttctcgtct gccagggcct gcacggaggg ctggtcatga gtgctcagga
      421 gctgtgcctg gatggtctcc accactcgct tgaaccgacg gctgggaccc gagatgagag
      481 tgaaggtgac ggagtagatg ccaccacctc cgctgccgtc ccgtcgcggg gagggctctg
      541 gaccctcaga ggagctgatg tccacctgga agcggacggg cttttggaag acggaggggc
      601 cgccactggc cttgtactcg gccctgaagc tggtctgtga cagcacactg tgactcaggc
      661 tggggatcga cagaaaggca tggacgatgt ctgctttgat gctgctgaga ggtttgtcct
      721 ttagcacgag gaatatttgt tcttctttgt ccaaggagat gaagttcccg aaccaggagc
      781 gttttgccag ctccggggag gactctggcg tcaagctgga catctcctca gcggtaggga
      841 cctgcatctt gcgccggtga aagcgagggg agcccaggaa gctgttgcgg atggagttga
      901 gacgactcct ccaggcggct cccccgacgc acgcggggct gggggggtgg tgttgtcccc
      961 ggggtcctgt gggactggcc tcgggcgtgt gcagagcgag tgcaaggggt cccgccagag
     1021 actccgggta gcctgcgggc tgtcaagggt tgtggatcgg cacttggggt gtg
//
