LOCUS       sequence-260428-        4815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC39A6
FEATURES             Location/Qualifiers
     source          1..4815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2940..3039)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3056..3075)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3057..3075)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3080..3096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3080..3096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3198..3217)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3209..4018
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3276..3295)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4165..4753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4654..4673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggcca ggaagctgtc tgtgatcctg atcctgacct tcgccctgag
      721 cgtgacaaat cctctgcacg agctgaaggc cgccgccttt ccccagacca cagagaagat
      781 ctcccctaat tgggagtctg gcatcaacgt ggatctggcc atcagcaccc ggcagtacca
      841 cctgcagcag ctgttctaca gatatggcga gaacaatagc ctgtccgtgg agggctttag
      901 gaagctgctg cagaacatcg gcatcgacaa gatcaagcgc atccacatcc accacgatca
      961 cgaccaccac agcgaccacg agcaccactc cgatcacgag cggcactctg accacgagca
     1021 ccacagcgag catgaacacc actccgatca cgaccaccac tctcaccaca atcacgccgc
     1081 cagcggcaag aacaagagaa aggccctgtg cccagatcac gactccgata gctccggcaa
     1141 ggatcccagg aattctcagg gcaagggagc acacaggcct gagcacgcat ccggccggag
     1201 aaatgtgaag gactctgtga gcgcctccga ggtgacctct acagtgtaca acaccgtgag
     1261 cgagggcaca cacttcctgg agaccatcga gacacctcgg ccaggcaagc tgtttccaaa
     1321 ggacgtgtct agctccaccc ccccttccgt gacatctaag agcagggtgt ctcgcctggc
     1381 cggcagaaag accaatgagt ccgtgtctga gcctcggaag ggcttcatgt atagcagaaa
     1441 cacaaatgag aacccacagg agtgttttaa cgccagcaag ctgctgacct cccacggcat
     1501 gggcatccag gtgccactga atgccacaga gttcaactac ctgtgccccg ccatcatcaa
     1561 tcagatcgat gcccggagct gtctgatcca cacctccgag aagaaggccg agatcccacc
     1621 caagacatat tccctgcaga tcgcctgggt gggcggcttc atcgccatca gcatcatctc
     1681 ctttctgtct ctgctgggcg tgatcctggt gcccctgatg aacagggtgt tctttaagtt
     1741 cctgctgagc tttctggtgg ccctggcagt gggcaccctg tccggcgacg ccttcctgca
     1801 cctgctgcct cacagccacg catcccacca ccacagccac tcccacgagg agccagccat
     1861 ggagatgaag cgcggccccc tgttcagcca cctgtctagc cagaatatcg aggagtctgc
     1921 ctactttgat agcacctgga agggactgac agccctggga ggactgtatt tcatgtttct
     1981 ggtggagcac gtgctgaccc tgatcaagca gtttaaggac aagaagaaga agaaccagaa
     2041 gaagcctgag aacgacgatg acgtggagat caagaagcag ctgtccaagt acgagtctca
     2101 gctgagcaca aacgaggaga aggtggatac cgatgacagg acagagggct atctgcgcgc
     2161 cgattcccag gagccttctc acttcgacag ccagcagcca gccgtgctgg aggaggagga
     2221 agtgatgatc gcccacgccc acccccagga ggtgtacaat gagtatgtgc ctagaggctg
     2281 caagaacaag tgtcacagcc actttcacga taccctgggc cagtccgatg acctgatcca
     2341 ccaccaccac gactaccacc acatcctgca tcaccaccac caccagaatc accacccaca
     2401 ctctcacagc cagcggtatt ccagagagga gctgaaggat gcaggagtgg ccacactggc
     2461 atggatggtc atcatgggcg atggcctgca caacttctct gacggactgg caatcggagc
     2521 agcctttacc gagggactgt cctctggcct gtccacatct gtggccgtgt tctgccacga
     2581 gctgccacac gagctgggcg actttgccgt gctgctgaag gccggcatga ccgtgaagca
     2641 ggccgtgctg tacaatgccc tgtctgccat gctggcctat ctgggcatgg ccaccggcat
     2701 cttcatcggc cactacgccg agaacgtgag catgtggatc ttcgccctga cagccggcct
     2761 gtttatgtat gtggccctgg tggacatggt gcccgagatg ctgcacaatg acgcctccga
     2821 tcacggctgt tctagatggg gctacttctt tctgcagaac gccggcatgc tgctgggctt
     2881 cggcatcatg ctgctgatct ccatctttga gcacaagatc gtgttccgca tcaactttga
     2941 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     3001 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     3061 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     3121 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     3181 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     3241 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     3301 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     3361 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     3421 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     3481 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     3541 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     3601 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     3661 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     3721 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     3781 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     3841 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     3901 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     3961 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     4021 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     4081 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     4141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4801 ttttgctcac atgtt
//
