LOCUS       sequence-260433-        4368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC26A11
FEATURES             Location/Qualifiers
     source          1..4368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2493..2592)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2609..2628)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2610..2628)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2633..2649)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2633..2649)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2751..2770)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2762..3571
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2829..2848)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3718..4306
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4207..4226
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgccca gctccgtgac cgccctggga caggcacggt ctagcggacc
      721 aggaatggca ccaagcgcct gctgttgctc ccctgccgcc ctgcagcgga gactgccaat
      781 cctggcatgg ctgcctagct actccctgca gtggctgaag atggacttcg tggcaggact
      841 gtccgtggga ctgaccgcaa tccctcaggc cctggcatac gcagaggtgg caggactgcc
      901 acctcagtac ggcctgtata gcgccttcat gggctgtttc gtgtacttct tcctgggcac
      961 ctccagagat gtgaccctgg gccccacagc catcatgtcc ctgctggtgt ctttctacac
     1021 atttcacgag cctgcctatg ccgtgctgct ggcctttctg agcggatgca tccagctggc
     1081 aatgggcgtg ctgaggctgg gcttcctgct ggactttatc tcctacccag tgatcaaggg
     1141 ctttacctct gccgcagcag tgactatagg ttttgggcaa atcaagaatc tgctgggcct
     1201 gcagaatatc cccagacctt tctttctgca ggtgtatcac accttcctga ggatcgccga
     1261 gacaagggtg ggcgacgccg tgctgggact ggtgtgcatg ctgctgctgc tggtgctgaa
     1321 gctgatgcgg gatcacgtgc cacccgtgca cccagagatg cctccaggcg tgaggctgtc
     1381 caggggactg gtgtgggcag caaccacagc aagaaacgca ctggtggtgt ctttcgccgc
     1441 cctggtggcc tacagctttg aggtgaccgg ctatcagccc ttcatcctga ccggagagac
     1501 agcagaggga ctgccacctg tgcggatccc accctttagc gtgaccacag ccaatggcac
     1561 catctccttc acagagatgg tgcaggacat gggagcagga ctggcagtgg tgcccctgat
     1621 gggcctgctg gagtctatcg ccgtggccaa ggcctttgcc agccagaaca attacaggat
     1681 cgatgccaac caggagctgc tggccatcgg cctgaccaat atgctgggct ccctggtgtc
     1741 ctcttatcca gtgaccggct cttttggcag gacagcagtg aacgcacaga gcggcgtgtg
     1801 caccccagca ggaggactgg tgacaggcgt gctggtgctg ctgtctctgg attacctgac
     1861 aagcctgttc tactatatcc ctaagagcgc cctggcagcc gtgatcatca tggcagtggc
     1921 accactgttc gacaccaaga tctttaggac actgtggcgg gtgaagagac tggatctgct
     1981 gcccctgtgc gtgaccttcc tgctgtgctt ttgggaggtg cagtatggaa tcctggcagg
     2041 cgccctggtg agcctgctga tgctgctgca ctccgccgca aggcctgaga caaaggtgtc
     2101 tgagggacca gtgctggtgc tgcagccagc atctggactg agcttcccag caatggaggc
     2161 cctgagggag gagatcctgt ccagagccct ggaggtgtct cctccacgct gcctggtgct
     2221 ggagtgtacc cacgtgtgca gcatcgacta cacagtggtg ctgggactgg gagagctgct
     2281 gcaggatttt cagaagcagg gagtggccct ggccttcgtg ggactgcagg tgcctgtgct
     2341 gcgcgtgctg ctgtccgccg acctgaaggg cttccagtac ttttctaccc tggaggaggc
     2401 cgagaagcac ctgaggcagg agcctggcac acagccatat aatatccgcg aggactccat
     2461 cctggatcag aaggtggccc tgctgaaggc cgacccagct ttcttgtaca aagttggcat
     2521 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     2581 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     2641 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     2701 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     2761 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     2821 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     2881 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     2941 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     3001 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     3061 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     3121 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     3181 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     3241 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     3301 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     3361 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     3421 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     3481 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     3541 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     3601 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     3661 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3721 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3781 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3841 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3901 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3961 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4021 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4081 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4141 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4201 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4261 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4321 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
