LOCUS       sequence-260749-       10627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KIF1A and cell fill TdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   McVicker DP, Awe AM, Richters KE, Wilson RL, Cowdrey DA, Hu X, 
            Chapman ER, Dent EW
  TITLE     Transport of a kinesin-cargo pair along microtubules into dendritic 
            spines undergoing synaptic plasticity.
  JOURNAL   Nat Commun. 2016 Sep 23;7:12741. doi: 10.1038/ncomms12741.
  PUBMED    27658622
REFERENCE   2  (bases 1 to 10627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-23"; volume: "7:12741. doi"; pages: " 
            10.1038/ncomms12741"
COMMENT     Sequence Label: tdTomato-KIF1A
FEATURES             Location/Qualifiers
     source          1..10627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        90..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      626..635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             632..2059
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     regulatory      3066..3075
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6647..6658
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7255..7271)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7256..7272)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7268..7320
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    7444..7565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7481..7500)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7535..7554
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7572..8027)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7709..7730)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7921..7940
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8054..8158
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8160..8517
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8160..8351
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8183..8203)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8368..8503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8552..9346
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8552..9346
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8606..8625)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9216..9235
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9534..9553)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9578..9625
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9954..10542
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10443..10462
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
      601 ttagtgaacc gtcagatccg ctagcgccac catggtgagc aagggcgagg aggtcatcaa
      661 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
      721 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      781 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
      841 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
      901 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
      961 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
     1021 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     1081 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
     1141 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
     1201 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
     1261 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac
     1321 cggcagcacc ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt
     1381 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
     1441 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     1501 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
     1561 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
     1621 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
     1681 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
     1741 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
     1801 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
     1861 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
     1921 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
     1981 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg
     2041 catggacgag ctgtacaagt tggcggccgc gactagtaca gatctcgaga tggctggggc
     2101 ctctgtgaag gtggcggtgc gcgtccgccc cttcaactcc cgggaaatga gccgcgactc
     2161 caagtgcatc attcagatgt ctggaaacac caccaccatt gtcaacccca aacagcccaa
     2221 ggagacaccc aagagcttca gcttcgatta ctcctactgg tcacatacct cgcccgagga
     2281 catcaactat gcctctcaga agcaggtcta cagggacatt ggggaggaga tgctacagca
     2341 tgcctttgag ggctacaacg tgtgcatctt tgcctatgga cagacagggg ctggcaagtc
     2401 ctacaccatg atgggcaaac aagagaagga ccagcaaggc atcatcccac agctctgtga
     2461 agacctcttc tctcgaatca atgacacgac caacgacaac atgtcctatt ctgtggaggt
     2521 cagctacatg gagatttact gtgagcgagt ccgcgacctc ctgaacccca agaacaaagg
     2581 caacctgcgt gtgagggagc accctctgct ggggccttat gtggaggacc tctccaagct
     2641 ggctgtgacc tcctacaatg acatccagga cctcatggac tcagggaaca aggccaggac
     2701 tgtggcggcc acaaacatga atgagaccag cagccgttct cacgccgtct tcaacatcat
     2761 cttcacccag aagcgccatg atgcggagac caacatcacc acagagaaag tgagcaaaat
     2821 cagcctggtg gacctggcgg gcagtgaacg agccgactcc acgggagcca agggcacacg
     2881 actcaaggag ggagccaaca tcaacaagtc tttgaccacc ctgggaaagg tcatctctgc
     2941 tctcgctgaa atggactctg gacccaataa gaacaagaaa aagaagaaga cagacttcat
     3001 cccttaccga gactcggtat tgacatggct tctccgggaa aacctgggcg gcaactcaag
     3061 gacagccatg gtggcagccc tgagccctgc ggacatcaac tatgatgaga ccctcagcac
     3121 actgagatat gcagaccggg ccaaacagat ccgctgcaac gccattatca atgaggaccc
     3181 taacaacaag ctgattcgag aactgaagga tgaggtgacc aggctgcgag atctgctgta
     3241 cgcccaaggt cttggcgata tcactgacac caacactgtg cctggaggac ccaaattgac
     3301 caatgccctg gtgggcatga gcccctcgtc ttcgctctca gccctgtcca gccgtgcggc
     3361 ctctgtgtcc agtctccacg agcgcatctt gtttgctcca ggcagcgagg aggctattga
     3421 aaggctgaag gaaacagaga agatcattgc tgagctcaat gagacgtggg aggagaagct
     3481 acggcggaca gaagccatcc ggatggagag ggaagccctg ctggccgaga tgggtgtggc
     3541 catgagggaa gatggtggca ccttgggagt gttttctccc aaaaagacac ctcatcttgt
     3601 caacctgaac gaggatcctt tgatgtctga atgcctcctt tactacatca aagatggcgt
     3661 caccagagtg ggcagagaag acgcagagag gcggcaagac attgtcctga gtgggcactt
     3721 catcaaggag gaacactgca tcttccggag cgactcccga ggaggcggag aagctgtggt
     3781 gaccctggag ccctgtgaag gagcagatac ctatgtgaac ggcaagaaag tcactgagcc
     3841 cagcatccta cggtcaggaa accgcatcat catgggaaaa agccacgtgt tccggtttaa
     3901 ccaccccgag caggctcgtc aggagcgtga gcggacacct tgtgcagaga cacccgctga
     3961 gccagtggac tgggcctttg cccagaggga gctgctggag aagcagggca ttgacatgaa
     4021 gcaggagatg gagcagaggc tccaggagct ggaggaccag taccggaggg agcgggagga
     4081 ggccacatac ctgctggagc agcagaggct ggactatgag agtaagctgg aggccctaca
     4141 gaaacagatg gactccaggt actacccaga ggtgaacgag gaggaggaag aaccagagga
     4201 tgaagtccaa tggacggagc gggagtgtga gttggcactg tgggccttcc ggaaatggaa
     4261 gtggtaccag ttcacttctc ttcgggacct gctgtggggc aatgccatct tcctgaagga
     4321 ggccaatgcc atcagtgtgg agctaaagaa gaaggtccag ttccagttcg tcctcctcac
     4381 agataccctc tactccccac tgccgccaga cctactcccc ccagaagctg ccaaagaccg
     4441 agagacacga cccttcccca ggaccatcgt ggccgtggag gtccaggacc agaagaatgg
     4501 ggccacccac tactggacgc tagagaaact caggcaacgc ctggacctga tgcgggaaat
     4561 gtatgaccgg gctgcagagg ttccctccag cgtcgttgag gactgtgaca atgtggtgac
     4621 tggtggagac cccttctatg accgcttccc ctggttccgg ctggtgggca gggccttcgt
     4681 atacctgagc aacttgctgt accctgtgcc cctggtgcac cgcgtggcca tcgtcagcga
     4741 aaagggagag gtgaaaggct tcctccgtgt ggcggtgcag gccatctcag ctgatgagga
     4801 ggctccggat tatggatcag gtgtccgtca gtcaggaacg gctaaaatct ccttcgatga
     4861 ccagcacttt gaaaagttcc agtctgagtc ctgccctgtg gtggggatgt cccgctcagg
     4921 gacctcccag gaggagctgc gcattgtaga aggccagggt cagggggctg atgctgggcc
     4981 ttcagccgat gaagtgaaca acaacacttg ctcagctgtg cctcctgaag gcctcctaga
     5041 tagcccagag aaggctgccc tggatggacc cctagacaca gccctggacc acctccgtct
     5101 gggcagtacc ttcaccttcc gggttacagt cctgcaggcg tccagtatct cggccgaata
     5161 tgctgacatc ttctgccagt tcaactttat ccaccgccac gatgaggcct tctccactga
     5221 acccctgaag aacactggca ggggaccccc tcttggcttc taccatgttc agaatattgc
     5281 agtagaggtg accaagtcct tcatcgaata catcaagagc cagcccattg tgttcgaggt
     5341 cttcgggcac taccaacagc atccattccc acctctttgc aaggatgttc tcagccccct
     5401 gaggccctca cgtcgccact tccccagggt catgccgcta tccaagccag tgcctgccac
     5461 taagctcagt acaatgacac ggccttcccc aggtccctgc cattgcaagt atgacctgct
     5521 ggtctacttc gagatctgcg agctggaagc caatggcgat tacatccctg ctgtggtaga
     5581 ccaccgtggc ggcatgccgt gcatggggac cttccttctc catcagggca tccagagacg
     5641 gataaccgtg accctgttgc atgagacagg aagccacatc cgctggaagg aagtgcgaga
     5701 gctggttgtg ggtcgcatcc gaaatactcc agaaactgac gaagctctga ttgaccccaa
     5761 catcttgtcc ctcaacatcc tgtcatcagg atacgtccat ccagcccagg atgaccggac
     5821 cttctaccag tttgaggcag cctgggacag ttccatgcat aactccctcc tgctgaatcg
     5881 agtcacccct taccgggaga aaatctacat gacactctct gcctacattg agatggagaa
     5941 ctgcacccag ccagctgtca tcactaagga cttctgcatg gttttctact cccgtgacgc
     6001 caagctgccc gcctcacgct ccattcgcaa cctgtttggc agtgggagcc tgagggccac
     6061 agaaggcaac cgtgtgactg gtgtgtatga gctcagcctt tgccatgtgg ccgatgcagg
     6121 cagcccaggc atgcagcgcc gccgcaggcg ggtgctggac acatcagtag cctacgtccg
     6181 tggtgaggag aacctggctg gctggaggcc ccggagtgac agcctgatcc tagaccacca
     6241 gtgggagctg gagaaactga gcctcttaca ggaggtggaa aagaccaggc actacctgct
     6301 cctacgggag aagctggaga ccacacagcg gcctgtgcct gaggtgctgt cgccagcttc
     6361 cagtgaggac tctgagtccc gtagctcttc aggtgcctct tcgccattgt cagccgaggg
     6421 tcagccgtca cctctggagg ttcccaatga gaggcagagg gagctggccg tcaagtgctt
     6481 acgactcctc atgcacacgt tcaacaggga gtacacccac agccacgtct gtatcagcgc
     6541 cagcgagagc aagctctcag agatgtctgt caccctgatg cgggacccat ccatgtctcc
     6601 tctgggtgcg gctacgctca ctccctcctc cacttgcccc tctctgattg aaggacgcta
     6661 tggtgccact gatgtgagga ccccccaacc ttgctcccgg cccgccagcc cagagcccga
     6721 gctccttcca gaacttgact ccaagaagac tccctcccct gttcgggcaa cagagacaga
     6781 gaaggagccc cagagactgc tggtccctga tatccaagag atccgagtca gcccaatcgt
     6841 ctccaagaag ggatacttgc acttcctgga gccacacaca gctggctggg ccaagcgctt
     6901 cgtggtggtg aggcgtccct atgcctacat gtacaacagt gacaaggaca cagtggagag
     6961 atttgtgctc aacctctcca cagcccaggt ggagtacagc gaggaccagc aggccatgct
     7021 caagacaccc aacacgttcg ctgtgtgcac ggagcaccga ggcatcctgc tgcaggccaa
     7081 tagtgacaag gacatgcacg actggctata tgccttcaac ccactcctgg ctgggaccat
     7141 tcggtccaag ctctcccgaa ggagatctgc gcagatgcgg gtctgagccg aaagccttcc
     7201 ggacttccag tggcccagac cactccctcg tgccgaattc gatatcaagc ttatcgatac
     7261 cgtcgacctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
     7321 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     7381 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     7441 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7561 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     7621 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     7681 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     7741 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     7801 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     7861 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     7921 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     7981 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     8041 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8101 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     8161 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8221 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8281 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8341 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8401 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     8461 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     8521 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     8581 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     8641 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     8701 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     8761 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     8821 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     8881 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     8941 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     9001 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     9061 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     9121 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     9181 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     9241 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     9301 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     9361 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     9421 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     9481 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     9541 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     9601 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     9661 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     9721 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     9781 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     9841 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9901 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9961 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10021 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10081 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10141 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10201 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10261 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10321 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10381 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10441 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10501 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10561 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10621 cctgatt
//
