LOCUS       sequence-260793-        3203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZE1-ahpCp-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Muller IE, Rubens JR, Jun T, Graham D, Xavier R, Lu TK
  TITLE     Gene networks that compensate for crosstalk with crosstalk.
  JOURNAL   Nat Commun. 2019 Sep 6;10(1):4028. doi: 10.1038/s41467-019-12021-y.
  PUBMED    31492904
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-6"; volume: "10(1):4028. doi"; pages: " 
            10.1038/s41467-019-12021-y"
COMMENT     Sequence Label: TJF66
FEATURES             Location/Qualifiers
     source          1..3203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             317..328
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             335..1045
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(480..498)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     748..767
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1081..1167
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1332..1920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1412..1431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2008..2102)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2132..2923,2924..2992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2755..2774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2993..3097)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3165..3183
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcttagatca ggtgattgcc ctttgtttat gagggtgttg taatccatgt cgttgttgca
       61 tttgtaaggg caacacctca gcctgcaggc aggcactgaa gataccaaag ggtagttcag
      121 attacacggt cacctggaaa gggggccatt ttacttttta tcgccgctgg cggtgcaaag
      181 ttcacaaagt tgtcttacga aggttgtaag gtaaaactta tcgatttgat aatggaaacg
      241 cattagccga atcggcaaaa attggttacc ttacatctca tcgaaaacac ggaggaagta
      301 tagatgcgaa ttcattaaag aggagaaagg taccatggtg agcaagggcg aagaagataa
      361 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      421 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      481 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      541 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      601 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      661 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      721 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      781 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      841 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      901 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      961 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     1021 cggcatggac gagctgtaca agtaacccgg gggatcccat ggtacgcgtg ctagaggcat
     1081 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1141 gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagggcgt tcggctgcgg
     1201 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1261 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1321 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1501 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1681 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1741 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     1801 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1861 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1981 gggattttgg tcatgactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc
     2041 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     2101 tccaagcgag ctcgtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2161 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2221 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2281 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2341 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2401 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2461 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2521 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2581 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2641 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2701 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2761 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2821 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2881 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2941 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3001 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3061 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3121 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3181 ggccctttcg tcttcacctc gag
//
