LOCUS       sequence-26080-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: 3xFRB-myc
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..44)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(45..323)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             complement(330..608)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
ORIGIN
        1 gggnctctag tctacagatc ctcttctgag atgagttttt gttcctttga gattcgtcgg
       61 aacacatgat aatagaggtc ccaggcttgg aggaggtcct tgacattccc tgatttcatg
      121 tacttcctgc accactcttg ggcctccatt aaatctcgac cataggcctg attaaaggat
      181 gtttccttca gagtctgggg gccccgttcc atcatagcat gcaagggctc cagcacctca
      241 aacatgcctt tcacgttcct ttccccaaag tacaaacgag atgcctcttc caggccttca
      301 tgccacatct catgccagag gattctagtc tttgagattc gtcggaacac atgataatag
      361 aggtcccagg cttgggtgag gtccttgaca ttccctgatt tcatgtactt cctgcaccac
      421 tcttgggcct ccattaaatc tcgaccatag gcctgattaa aggatgtttc cttcagagtc
      481 tgggggcccc gttccatcat agcatgcaag ggctccagca cctcaaacat gcctttcacg
      541 ttcctttccc caaagtacaa acgagatgcc tcttccaggc cttcatgcca catctcatgc
      601 cagaggattc tagtctttga gattcgtcgg aacacatgat aatagaggtc ccaggcttgg
      661 gtgaggtcct tgacattccc tgatttcatg tacttcctgc accactcttg ggcctccatt
      721 anatnncagt nnnnnnnnnt catnannaan nngntnnncn nnnnnngtcn gggggcccnn
      781 ttcnacntna nanncangca agggctccag cacctcaaac atgcc
//
