LOCUS       sequence-26081-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: 2xFRB-myc
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..42)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(43..321)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             complement(328..606)
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     gap             642..677
                     /estimated_length=36
ORIGIN
        1 gcctctagtc tacagatcct cttctgagat gagtttttgt tcctttgaga ttcgtcggaa
       61 cacatgataa tagaggtccc aggcttggag gaggtccttg acattccctg atttcatgta
      121 cttcctgcac cactcttggg cctccattaa atctcgacca taggcctgat taaaggatgt
      181 ttccttcaga gtctgggggc cccgttccat catagcatgc aagggctcca gcacctcaaa
      241 catgcctttc acgttccttt ccccaaagta caaacgagat gcctcttcca ggccttcatg
      301 ccacatctca tgccagagga ttctagtctt tgagattcgt cggaacacat gataatagag
      361 gtcccaggct tgggtgaggt ccttgacatt ccctgatttc atgtacttcc tgcaccactc
      421 ttgggcctcc attaaatctc gaccataggc ctgattaaag gatgtttcct tcagagtctg
      481 ggggccccgt tccatcatag catgcaaggg ctccagcacc tcaaacatgc ctttcacgtt
      541 cctttcccca aagtacaaac gagatgcctc ttccaggcct tcatgccaca tctcatgcca
      601 gaggattcta gactcgagcg gccgccactg tgctggatat cnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnann nnnnnnnggc gccncgcgan natatattna nnnataattc
      721 gatagcagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
      781 ncc
//
