LOCUS       sequence-261142-        4725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC9A7
FEATURES             Location/Qualifiers
     source          1..4725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2850..2949)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2966..2985)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2967..2985)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2990..3006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2990..3006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3108..3127)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3119..3928
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3186..3205)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4075..4663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4564..4583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggagc ctggcgatgc cgccaggcca ggaagcggaa gggcaaccgg
      721 agcaccccct ccaaggctgc tgctgctgcc actgctgctg ggatggggac tgagagtggc
      781 agcagcagcc tccgccagct cctctggcgc cgccgccgag gatagctccg ccatggagga
      841 gctggccacc gagaaggagg ccgaggagtc tcacaggcag gactctgtga gcctgctgac
      901 attcatcctg ctgctgaccc tgacaatcct gaccatctgg ctgtttaagc accggagagt
      961 gcgcttcctg cacgagaccg gactggcaat gatctacggc ctgatcgtgg gcgtgatcct
     1021 gagatatgga accccagcca catccggaag ggataagtcc ctgtcttgca cccaggagga
     1081 cagggccttt tccacactgc tggtgaacgt gtctggcaag ttctttgagt acacactgaa
     1141 gggcgagatc tctcccggca agatcaacag cgtggagcag aatgatatgc tgcgcaaggt
     1201 gaccttcgac cctgaggtgt tctttaatat cctgctgccc cctatcatct ttcacgccgg
     1261 ctactccctg aagaagcggc acttctttag aaacctgggc tctatcctgg cctatgcctt
     1321 tctgggcaca gccgtgagct gcttcatcat cggcaatctg atgtacggcg tggtgaagct
     1381 gatgaagatc atgggccagc tgtccgataa gttctactat accgactgtc tgttctttgg
     1441 cgccatcatc tctgccaccg accccgtgac agtgctggcc atcttcaacg agctgcacgc
     1501 cgacgtggat ctgtatgccc tgctgtttgg cgagagcgtg ctgaatgatg ccgtggccat
     1561 cgtgctgtct agctccatcg tggcatacca gcctgcaggc ctgaacaccc acgccttcga
     1621 cgcagccgcc ttctttaagt ccgtgggcat cttcctgggc atctttagcg gctccttcac
     1681 catgggcgcc gtgacaggcg tgaacgccaa tgtgaccaag tttacaaagc tgcactgttt
     1741 ccctctgctg gagaccgccc tgttctttct gatgtcttgg agcacatttc tgctggcaga
     1801 ggcatgcggc ttcaccggag tggtggccgt gctgttttgt ggcatcaccc aggcccacta
     1861 cacatataac aatctgagcg tggagtccag gtctcgcaca aagcagctgt ttgaggtgct
     1921 gcacttcctg gccgagaatt tcatctttag ctatatgggc ctggccctgt tcacctttca
     1981 gaagcacgtg ttttccccta tcttcatcat cggcgccttt gtggcaatct tcctgggaag
     2041 ggcagcacac atctacccac tgtccttctt tctgaacctg ggcaggcgcc acaagatcgg
     2101 ctggaatttt cagcacatga tgatgttcag cggactgaga ggagcaatgg ccttcgccct
     2161 ggcaatcagg gatacagcct cctatgccag acagatgatg tttaccacaa ccctgctgat
     2221 cgtgttcttt accgtgtgga tcatcggcgg cggcacaacc ccaatgctgt cctggctgaa
     2281 catcagggtg ggagtggagg agccatctga ggaggatcag aatgagcacc actggcagta
     2341 cttcagagtg ggagtggacc cagatcagga cccaccacct aacaatgact cttttcaggt
     2401 gctgcagggc gatggaccag acagcgccag gggaaacaga acaaagcagg agagcgcctg
     2461 gatctttcgg ctgtggtact ccttcgatca caattatctg aagcctatcc tgacccacag
     2521 cggcccaccc ctgacaacca cactgccagc atggtgcgga ctgctggcaa ggtgtctgac
     2581 atctccccag gtgtatgaca accaggagcc tctgcgcgag gaggatagcg acttcatcct
     2641 gaccgagggc gatctgaccc tgacatacgg cgactctacc gtgacagcca atggctctag
     2701 ctcctctcac accgcaagca catccctgga gggaagccgg agaacaaaga gctcctctga
     2761 ggaggtgctg gagcgggatc tgggcatggg cgaccagaag gtgagctcca ggggaaccag
     2821 gctggtgttc ccactggagg acaacgccga cccagctttc ttgtacaaag ttggcattat
     2881 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2941 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     3001 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     3061 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     3121 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     3181 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     3241 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     3301 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     3361 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     3421 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     3481 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     3541 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     3601 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3661 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3721 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3781 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3841 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3901 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3961 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     4021 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4081 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4141 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4201 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4261 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4321 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4381 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4441 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4501 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4561 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4621 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4681 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
