LOCUS       sequence-261183-       10233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ATP measurement, nucleus, Tol2 transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Yaginuma H,  Okada Y
  TITLE     Live cell imaging of metabolic heterogeneity by quantitative 
            fluorescent ATP indicator protein, QUEEN-37C
  JOURNAL   bioRxiv 2021.10.08.463131
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.10.08.463131"
COMMENT     Sequence Label: pT2MPEF-NLS-QUE37C
FEATURES             Location/Qualifiers
     source          1..10233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..1182
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          231..1173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1245..1265
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      1278..1287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1644..1895
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1832..1853
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1959..1980)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2220..2239)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2483..2707
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2754..2777)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3151..3170
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3235..3834
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3235..3254)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3731..3751
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    3921..4145
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4173..4189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4197..4213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4221..4227,4228..4245,4246..4251))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4266..4287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(5603..5740,5741..5752)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(5771..5789)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5810..5826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5834..5850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5858..5864,5865..5882,5883..5888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5903..5924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6041..6058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6212..6800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6292..6311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6971..7762,7763..7831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7594..7613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7832..7936)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7962..8417)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8099..8120)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8311..8330
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8544..8566
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8558..8575
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8559..8575
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8582..8601
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8582..8600
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(8614..8625,8626..8813)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    10082..10130
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattaa agctagcgct
     1201 accggactca gatctcgagc tcaagcttcg aattcaccat ggcgccgaaa aaaaaacgca
     1261 aagtggatcc accggtcgcc accatggtga aaactgtgaa agtgaatata acaacccctg
     1321 atgggccagt ctacgacgct gatatcgaga tggtgtccgt gcgggccgag agtggtgatc
     1381 tcggcatcct ccccggtcac attcccacaa aggccccact gaagatcgga gctgtgcggc
     1441 tgaagaagga cggccaaaca gaggatgtcg cagtctcagg cggcactgtt gaagtgcggc
     1501 ctgaccacgt taccattaat gctcaagccg ctgaacgggc ggaggacatt gacgtcctcc
     1561 gcgccaaagc ggcgaaagag cgggcggagc gccgcctgca aagccagacc cgttacaaca
     1621 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1681 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1741 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc
     1801 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1861 ccgggatcac tctcggcatg gacgagctgt acaagggtgg caccggaggg agcatggtga
     1921 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1981 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     2041 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     2101 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2161 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2221 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2281 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2341 agtacaacct gggtgacatc gacttcaaac gggccgaact ggcgttaaaa cgcgccatga
     2401 accgtttgag cgttgcggaa gataagtgag cggccgctcg ataagcttga tcgatgatct
     2461 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2521 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2581 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2641 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2701 tctatggctt ctgaggcgga aagaacctgc agcttttccc aaggcagtct ggagcatgcg
     2761 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     2821 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     2881 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     2941 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     3001 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     3061 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     3121 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     3181 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgtggccac aaccatgacc
     3241 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     3301 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     3361 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     3421 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     3481 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     3541 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     3601 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     3661 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     3721 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     3781 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     3841 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gatgatctag
     3901 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3961 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4021 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4081 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4141 tatggctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4201 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4261 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4321 ggaaacctgt cgtgccagcg gatccctaga gggacagccc ccccccaaag cccccaggga
     4381 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
     4441 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
     4501 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
     4561 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
     4621 agaacggcct gggttgcaaa ggagcacagt gctcatccag atccaacccc ctgctatgtg
     4681 cagggtcatc aaccagcagc ccaggctgcc cagagccaca tccagcctgg ccttgaatgc
     4741 ctgcagggat ggggcatcca cagcctcctt gggcaacctg ttcagtgcgt caccaccctc
     4801 tgggggaaaa actgcctcct catatccaac ccaaacctcc cctgtctcag tgtaaagcca
     4861 ttcccccttg tcctatcaag ggggagtttg ctgtgacatt gttggtctgg ggtgacacat
     4921 gtttgccaat tcagtgcatc acggagaggc agatcttggg gataaggaag tgcaggacag
     4981 catggacgtg ggacatgcag gtgttgaggg ctctgggaca ctctccaagt cacagcgttc
     5041 agaacagcct taaggataag aagataggat agaaggacaa agagcaagtt aaaacccagc
     5101 atggagagga gcacaaaaag gccacagaca ctgctggtcc ctgtgtctga gcctgcatgt
     5161 ttgatggtgt ctggatgcaa gcagaagggg tggaagagct tgcctggaga gatacagctg
     5221 ggtcagtagg actgggacag gcagctggag aattgccatg tagatgttca tacaatcgtc
     5281 aaatcatgaa ggctggaaaa gccctccaag atccccaaga ccaaccccaa cccacccacc
     5341 gtgcccactg gccatgtccc tcagtgccac atccccacag ttcttcatca cctccaggga
     5401 cggtgacccc cccacctccg tgggcagctg tgccactgca gcaccgctct ttggagaagg
     5461 taaatcttgc taaatccagc ccgaccctcc cctggcacaa cgtaaggcca ttatctctca
     5521 tccaactcca ggacggagtc agtgaggatg gggctctagc cggaccgacg cgtctcgata
     5581 gggataacag ggtaattgat ccaatactca agtacaattt taatggagta cttttttact
     5641 tttactcaag taagattcta gccagatact tttactttta attgagtaaa attttcccta
     5701 agtacttgta ctttcacttg agtaaaattt ttgagtactt tttacacctc tggagctcca
     5761 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     5821 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5881 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5941 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6001 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6061 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6121 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6181 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6241 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6301 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6361 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6421 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6481 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6541 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6601 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6661 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6721 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6781 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6841 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6901 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6961 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7021 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7081 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7141 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7201 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7261 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7321 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7381 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7441 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7501 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7561 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7621 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7681 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7741 ctttcaccag cgtttctggg tgagcaaaaa cgggaaggca aaatgccgca aaaaagggaa
     7801 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7861 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7921 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     7981 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     8041 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     8101 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     8161 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     8221 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     8281 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     8341 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     8401 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     8461 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     8521 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     8581 gtaatacgac tcactatagg gcgaattggg taccagaggt gtaaagtact tgagtaattt
     8641 tacttgatta ctgtacttaa gtattatttt tggggatttt tactttactt gagtacaatt
     8701 aaaaatcaat acttttactt ttacttaatt acattttttt agaaaaaaaa gtacttttta
     8761 ctccttacaa ttttatttac agtcaaaaag tacttatttt ttggagatca cttgtcgagt
     8821 agggataaca gggtaatgat cccccgggct gcagtcgcga cgcgtcctag agggacagcc
     8881 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     8941 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     9001 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     9061 gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg ctgaaagaga
     9121 gatttagaat gacagaatca tagaacggcc tgggttgcaa aggagcacag tgctcatcca
     9181 gatccaaccc cctgctatgt gcagggtcat caaccagcag cccaggctgc ccagagccac
     9241 atccagcctg gccttgaatg cctgcaggga tggggcatcc acagcctcct tgggcaacct
     9301 gttcagtgcg tcaccaccct ctgggggaaa aactgcctcc tcatatccaa cccaaacctc
     9361 ccctgtctca gtgtaaagcc attccccctt gtcctatcaa gggggagttt gctgtgacat
     9421 tgttggtctg gggtgacaca tgtttgccaa ttcagtgcat cacggagagg cagatcttgg
     9481 ggataaggaa gtgcaggaca gcatggacgt gggacatgca ggtgttgagg gctctgggac
     9541 actctccaag tcacagcgtt cagaacagcc ttaaggataa gaagatagga tagaaggaca
     9601 aagagcaagt taaaacccag catggagagg agcacaaaaa ggccacagac actgctggtc
     9661 cctgtgtctg agcctgcatg tttgatggtg tctggatgca agcagaaggg gtggaagagc
     9721 ttgcctggag agatacagct gggtcagtag gactgggaca ggcagctgga gaattgccat
     9781 gtagatgttc atacaatcgt caaatcatga aggctggaaa agccctccaa gatccccaag
     9841 accaacccca acccacccac cgtgcccact ggccatgtcc ctcagtgcca catccccaca
     9901 gttcttcatc acctccaggg acggtgaccc ccccacctcc gtgggcagct gtgccactgc
     9961 agcaccgctc tttggagaag gtaaatcttg ctaaatccag cccgaccctc ccctggcaca
    10021 acgtaaggcc attatctctc atccaactcc aggacggagt cagtgaggat ggggctctag
    10081 gaataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg gtcgacattg
    10141 attattgact agatcatcgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
    10201 cccacagtcc ccgagaagtt ggggggaggg gtc
//
