LOCUS       sequence-261238-       12303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HDR-cassette to target chicken GAPDH gene for EGFP expression under 
            control of the endogenous promoter. The cassette contains NeoR gene 
            for positive clone selection and DTA gene for negative selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12303)
  AUTHORS   Antonova E, Glazova O, Gaponova A, Eremyan A, Zvereva S, Grebenkina 
            N, Volkova N, Volchkov P
  TITLE     Successful CRISPR/Cas9 mediated homologous recombination in a 
            chicken cell line.
  JOURNAL   F1000Res. 2018 Feb 28;7:238. doi: 10.12688/f1000research.13457.2. 
            eCollection 2018.
  PUBMED    29946437
REFERENCE   2  (bases 1 to 12303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2018-02-28"; volume: "7:238. doi"; pages: " 
            10.12688/f1000research.13457.2. eCollection 2018"
COMMENT     Sequence Label: KI(GAPDH)-P2A-EGFP-CMV-NeoR
FEATURES             Location/Qualifiers
     source          1..12303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1465..1521
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1522..1524,1525..2238)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1522..1524,1525..2235)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1564..1585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1825..1844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2172..2193
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2289..2877
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2342..2362)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2760..2771)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2880..2896)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2904..3111
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3119..3152
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     enhancer        3171..3474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3475..3678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3629..3649
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3761..4564
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3824..3843)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4434..4453
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4579..4700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4616..4635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4670..4689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        7855..8354
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7890..7913)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8287..8306
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8381..8953
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          9030..9095
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    9169..9393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9455..9475)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9455..9473)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9494..9510)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9494..9510)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9507..9529)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9518..9534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9542..9548,9549..9566,9567..9572))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9587..9608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9725..9742)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9896..10484)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9976..9995)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10655..11446,11447..11515))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11278..11297
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11516..11620)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11647..12102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11734..11753)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11944..11965
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12228..12250
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12242..12259
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12243..12259
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12269..12288
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12269..12287
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 gggcagcgga ggagcgcttc cggccgacgt ctcgtcgctg attggcttct tttcctcccg
      241 ccgtgtgtga aaacacaaat ggcgtgtttt ggttggcgta aggcgcctgt cagttaacgg
      301 cagccggagt gcgcagccgc cggcagcctc gctctgccca ctgggtgggg cgggaggtag
      361 gtggggtgag gcgagctgga cgtgcgggcg cggtcggcct ctggcggggc gggggagggg
      421 agggagggtc agcgaaagta gctcgcgcgc gttgttgacc tgacctgccg tctggagaaa
      481 ccagtaagta ttgtggataa ggctgaaaaa tcttcaggct tgcctctaaa attctccata
      541 catgaaatta cgattcctac atccatctgt tcaatagaaa aacttgtttt tgctgaaaat
      601 agtacttgat atgcatggct agtgctatgc agttcccagt gtctgggctg tagctgacat
      661 tttgagatgg gatttgacca tcttctgcct tttttcaggc caagtatgat gatatcaaga
      721 gggtagtgaa ggctgctgct gatgggcccc tgaagggcat cctaggatac acagaggacc
      781 aggtaagtac tttctatgta ggtgttactt gcctcatttt tctgatggtg gcagccttgc
      841 tgggatgatg tttttgggta cttctggttc aggctgttat tgtctgttga gtggaagagt
      901 gtttgacagt gtctctctgt tccaggttgt ctcctgtgac ttcaatggtg acagccattc
      961 ctccaccttt gatgcgggtg ctggcattgc gctgaatgac catttcgtca agcttgtttc
     1021 ctggtatgca cagcctcggg gtgtggggga gactgggagg tagaggctgg cagttctggt
     1081 gcggttctgt gctgtttcat ttcatcagca ccatctgaca atgcatggca acacagatcc
     1141 ttgctgtgtg catgcggttc cagcatgtga cagtctctag tgagatggct tcgtatctga
     1201 aatggctgca gctgccttcc tgtaatggag ggcagatggg atgctaccta ctttctttat
     1261 aaggaataga tgggaagctg tcccatttgg ttgtatgggc tgggactgga agagcagtaa
     1321 catgaaaagg gatacaagaa acccagtcag tgctgacagc acttctttcc cttcctcagg
     1381 tatgacaatg agtttggata cagcaaccgt gttgtggact tgatggtcca catggcatcc
     1441 aaggagggtt taaacggaag cggagccact aacttctccc tgttgaaaca agcaggggat
     1501 gtcgaagaga atcccgggcc agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1561 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1621 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1681 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     1741 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1801 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1861 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1921 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1981 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2041 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2101 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     2161 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2221 gacgagctgt acaagtaaac gcgtatgcat ggccggccct gcaggaattc gatatcaagc
     2281 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2341 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2401 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2461 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2521 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2581 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2641 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2701 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2761 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2821 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     2881 gataccgtcg acctcgacct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2941 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3001 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3061 gcaggacagc aagggggagg attgggaaga caatggcagg catgctgggg aactagtggt
     3121 gccagggcgt gcccttgggc tccccgggcg cggcggccgc tggagttccg cgttacataa
     3181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     3241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     3301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     3361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     3421 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     3481 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     3541 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     3601 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     3661 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag
     3721 atctcgagct caagcttcga attctgcagt cgaccgccac atgggatcgg ccattgaaca
     3781 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     3841 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     3901 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     3961 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     4021 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     4081 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     4141 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     4201 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     4261 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct
     4321 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     4381 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     4441 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     4501 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     4561 ctgacaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4621 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4681 aaactcatca atgtatctta gggcgcgccc cctttgttgg agcccctgct cttcaccacc
     4741 gctcagttct gcatcctgca gtgagaggcc agttctgttc ccttctgtct cccccactcc
     4801 tccaatttct tcctccacct gggggaggtg ggagaggctg atagaaactg atctgtttgt
     4861 gtaccacctt acatcaataa aagtgttcac catctgaagt gccttgtttg tgtgtcattg
     4921 ggaaactaaa gggaattcaa taaatgcttt gaattaaatt caataaatgc ttacggccac
     4981 cctgtaactg tggggccagc aactccagca agtgttctgc ggtgttccat acctgctgct
     5041 ccactgacaa cacgttaagg ctacaagccc tggcatcagg tgctggcatt tggataattc
     5101 tggttgtgca gaagtgtgga gctgtcctgg gatctatgat ctgtaaaagc agtcactgcc
     5161 cagctgtact gagtgtgagg ctgctgtgca tggagctgtg gcctgcttgc tggcaaggga
     5221 agggctcctg cccttgagcc aaaaggtgtg ttggagggct gtgactggaa ggctgccgca
     5281 tggtggaatg gaaagcagct gttcatccac cttccagctc tctggatttt cttgtctgat
     5341 gggtgtgtgc agaataaagt gctgaggagt gactttgcat tttgacatgg caaaatggca
     5401 caaaacccag gatttttttt attccccctt cgttactaga gatgctacaa agagaactgt
     5461 tctactgcat agctgaaaat aaatcaccct ggaagaaatg gctgcctctg gccctgtgtt
     5521 ctgtggtggg agctagaatt aagcacagct ctgactgcag gagtggcagc tgttgtcagg
     5581 ctcatgagca gcaaatctct gcaagtatca gccctctgcc aggagctgca ttagctccct
     5641 ctgaaagtgc tgaatgctgc agggcgggag cctaccctgg atgatgagtg gtagggtaga
     5701 taggcagttc actaccacca aaaccgcggg gtccttcctt tttttttcat cactaatgag
     5761 gctgagagct gcatcagaga tactgcattg ctccctgcag gaggcaggtt gcagctgctg
     5821 actggagacg gctgctttta cagtctgacc tggggcaagg accgaggctg gaggctctcc
     5881 catggccgag acgtgagaaa catgggacag tctcctggag ccgcttatct ctgacggtag
     5941 agctgtgtga aaactagacg ttcgctgcgc tagagggaga tgactcagag tcgttttcct
     6001 tagaggaaat aggttgcata acctgtctct ggacttacag tacgaacagc tgattggctc
     6061 tgggatggcc tcaatacctg gcttgtcctc taaatgtcct gacagaagct ttaggtaact
     6121 ccagagtgaa actgcagctg ctcttcagct ttcagctgct ctggggctgg ccaggggggc
     6181 agccctgctg agcaggtggg atgctgcagt gcagcaggca gcctacagcc ctgccaggct
     6241 aacaaagagc cctgtgtggg tcccatcctg gccataattg ctgtgctcgg tgctttgaat
     6301 ggctgtcctg tgtgccgctg cctgcatagt tgtgtgcatg ccagcaagat ccttgtgtga
     6361 tgatagggaa gtgctgagag gctgcagaaa caccagatgc ccgcaagcag gaacatttta
     6421 aagaatcacc tttttcatct aagaagcctt atgtttcctg agcagtctcc agctgcctga
     6481 aaaatgcatt gaactcatgc tgttattcaa caaagcccta tcaaattgct gtgggagtgg
     6541 ggggcaaatt aatacaacct gggggctgtg ggggacaggg aggggaggca gagtacctgt
     6601 tgttctgaga accactgctg cgaacgtttt gggtataacg gttgattttg ttttcattaa
     6661 aacaccgttc caacccgttc acactcttgc tggatgttcc cgtaaggcta cgggcagcga
     6721 ggctgacgaa gccgggagcc gcagaactta cagcgcggta gctcaggtgt ggaagtagtg
     6781 agagggcgct tggccaagcg agctgtgcag cagccccagt ccgctgcggg ctcggtgccc
     6841 tccctctctt catcagcccc cggcaccttc ccgtgagtcc tgaagagttt gcactcagtc
     6901 gctgcgcctg cagatggcac cacagcaagg ccacgggcgg cagagctgtg tcaccgctgt
     6961 gtcaccgctc tgccgccctg tccaggagct gcagccctga agaggggagc gctgctgttg
     7021 cagctgactc tctccctgta gaagttctcg gctcagcaga gaagctctgg gtttgctctt
     7081 gcacattttg aaggaaatct tgcagaaacg atcactgggg gggagagggg tggacaggct
     7141 ctcgggctgg cagaaggagg ctctgcccag cagaggagat ggaagaaatg gtgctgctgt
     7201 gccaggccaa accagctgct agggcagctg cagtcagcca gaacccatgc tactctatga
     7261 gcgcccaagg cagcactcag tgctgctcgg ggctgtgccg gcacagcaca agggccatgc
     7321 ctcagagtgg cagccaacta catccagggg tgctcagttg cacctttttg ttcttatgaa
     7381 ggcagagaaa attcagaatg aagcagtggt atggtaatta gcagtgcttt tagagcaaaa
     7441 gcgctgcttt tttggcagtt tcaaatttca agagagagaa tctcatcggc atttgcacgc
     7501 cttaccatac gtgtgttgcc tcccaagaaa tggctggggt tgttctcaga ccttctcaag
     7561 atgtcagcat gagaccgtag ccttaagcag aacattatgt agtgaaaacc atcttgagca
     7621 gacgtgcaaa ctaactaatg ctactggaac gtcacaccga aatggaacaa aacagccact
     7681 acacaagcac agtaaaagtg ttggacaccc acacatactg tctgcttgta aagtaaaccg
     7741 cttgctgagc acatcactcc aaaaggtctg tgcaaggaag atacagggct cggagagaaa
     7801 agactgcgag gcatgtcgtc gacggtatcg ataagcttga tatcgaattc taccgggtag
     7861 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     7921 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     7981 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     8041 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     8101 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     8161 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     8221 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     8281 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     8341 ctccgggcct ttcgacctgc aggtcctcgc catggatcct gatgatgttg ttgattcttc
     8401 taaatctttt gtgatggaaa acttttcttc gtaccacggg actaaacctg gttatgtaga
     8461 ttccattcaa aaaggtatac aaaagccaaa atctggtaca caaggaaatt atgacgatga
     8521 ttggaaaggg ttttatagta ccgacaataa atacgacgct gcgggatact ctgtagataa
     8581 tgaaaacccg ctctctggaa aagctggagg cgtggtcaaa gtgacgtatc caggactgac
     8641 gaaggttctc gcactaaaag tggataatgc cgaaactatt aagaaagagt taggtttaag
     8701 tctcactgaa ccgttgatgg agcaagtcgg aacggaagag tttatcaaaa ggttcggtga
     8761 tggtgcttcg cgtgtagtgc tcagccttcc cttcgctgag gggagttcta gcgttgaata
     8821 tattaataac tgggaacagg cgaaagcgtt aagcgtagaa cttgagatta attttgaaac
     8881 ccgtggaaaa cgtggccaag atgcgatgta tgagtatatg gctcaagcct gtgcaggaaa
     8941 tcgtgtcagg cgatctcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     9001 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     9061 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     9121 gggagcagtg gtggaatgca gatcctagag ctcgctgatc agcctcgact gtgccttcta
     9181 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     9241 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     9301 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     9361 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     9421 gctcgagggg gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     9481 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9541 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9601 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9661 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     9721 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9781 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9841 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10021 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10081 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10141 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10201 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10261 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10321 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    10381 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    10441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    10621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    10681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    10741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    10801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    11341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    11401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    11461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    11521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    11581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    11641 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    11701 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    11761 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    11821 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    11881 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    11941 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    12001 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    12061 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
    12121 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    12181 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    12241 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct
    12301 cca
//
