LOCUS       sequence-261457-       11551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human LMNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Earle AJ, Kirby TJ, Fedorchak GR, Isermann P, Patel J, Iruvanti S, 
            Moore SA, Bonne G, Wallrath LL, Lammerding J
  TITLE     Mutant lamins cause nuclear envelope rupture and DNA damage in 
            skeletal muscle cells.
  JOURNAL   Nat Mater. 2019 Dec 16. pii: 10.1038/s41563-019-0563-5. doi: 
            10.1038/s41563-019-0563-5.
  PUBMED    31844279
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Mater. 
            2019 Dec 16. pii: 10.1038/s41563-019-0563-5. doi: 
            10.1038/s41563-019-0563-5."
COMMENT     Sequence Label: pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro
FEATURES             Location/Qualifiers
     source          1..11551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..1984
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1981..2005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4083..4655
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4106..4657
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4250..4267)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             4681..5343
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     ISPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     promoter        5461..5672
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5685..5953
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5982..5991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6012..6674
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6744..6797
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6798..7397
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6798..6817)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7294..7314
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7398..7986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7451..7471)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7869..7880)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8060..8293
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8365..8486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8402..8421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8456..8475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8526..8661
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8694..8710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8694..8710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8707..8729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8718..8734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8742..8748,8749..8766,8767..8772))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8787..8808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8925..8942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9096..9684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9855..10646,10647..10715))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10478..10497
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10716..10820)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10888..10906
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10944..10966)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11066..11085
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11279..11301
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11293..11310
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11294..11310
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11325..11551
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgatact agtattatgc ccagtacatg accttatggg actttcctac
     1741 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1801 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1861 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1921 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag
     1981 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2041 tagaagattc tagagctagc gaattctaac catggagacc ccgtcccagc ggcgcgccac
     2101 ccgcagcggg gcgcaggcca gctccactcc gctgtcgccc acccgcatca cccggctgca
     2161 ggagaaggag gacctgcagg agctcaatga tcgcttggcg gtctacatcg accgtgtgcg
     2221 ctcgctggaa acggagaacg cagggctgcg ccttcgcatc accgagtctg aagaggtggt
     2281 cagccgcgag gtgtccggca tcaaggccgc ctacgaggcc gagctcgggg atgcccgcaa
     2341 gacccttgac tcagtagcca aggagcgcgc ccgcctgcag ctggagctga gcaaagtgcg
     2401 tgaggagttt aaggagctga aagcgcgcaa taccaagaag gagggtgacc tgatagctgc
     2461 tcaggctcgg ctgaaggacc tggaggctct gctgaactcc aaggaggccg cactgagcac
     2521 tgctctcagt gagaagcgca cgctggaggg cgagctgcat gatctgcggg gccaggtggc
     2581 caagcttgag gcagccctag gtgaggccaa gaagcaactt caggatgaga tgctgcggcg
     2641 ggtggatgct gagaacaggc tgcagaccat gaaggaggaa ctggacttcc agaagaacat
     2701 ctacagtgag gagctgcgtg agaccaagcg ccgtcatgag acccgactgg tggagattga
     2761 caatgggaag cagcgtgagt ttgagagccg gctggcggat gcgctgcagg aactgcgggc
     2821 ccagcatgag gaccaggtgg agcagtataa gaaggagctg gagaagactt attctgccaa
     2881 gctggacaat gccaggcagt ctgctgagag gaacagcaac ctggtggggg ctgcccacga
     2941 ggagctgcag cagtcgcgca tccgcatcga cagcctctct gcccagctca gccagctcca
     3001 gaagcagctg gcagccaagg aggcgaagct tcgagacctg gaggactcac tggcccgtga
     3061 gcgggacacc agccggcggc tgctggcgga aaaggagcgg gagatggccg agatgcgggc
     3121 aaggatgcag cagcagctgg acgagtacca ggagcttctg gacatcaagc tggccctgga
     3181 catggagatc cacgcctacc gcaagctctt ggagggcgag gaggagaggc tacgcctgtc
     3241 ccccagccct acctcgcagc gcagccgtgg ccgtgcttcc tctcactcat cccagacaca
     3301 gggtgggggc agcgtcacca aaaagcgcaa actggagtcc actgagagcc gcagcagctt
     3361 ctcacagcac gcacgcacta gcgggcgcgt ggccgtggag gaggtggatg aggagggcaa
     3421 gtttgtccgg ctgcgcaaca agtccaatga ggaccagtcc atgggcaatt ggcagatcaa
     3481 gcgccagaat ggagatgatc ccttgctgac ttaccggttc ccaccaaagt tcaccctgaa
     3541 ggctgggcag gtggtgacga tctgggctgc aggagctggg gccacccaca gcccccctac
     3601 cgacctggtg tggaaggcac agaacacctg gggctgcggg aacagcctgc gtacggctct
     3661 catcaactcc actggggaag aagtggccat gcgcaagctg gtgcgctcag tgactgtggt
     3721 tgaggacgac gaggatgagg atggagatga cctgctccat caccaccacg gctcccactg
     3781 cagcagctcg ggggaccccg ctgagtacaa cctgcgctcg cgcaccgtgc tgtgcgggac
     3841 ctgcgggcag cctgccgaca aggcatctgc cagcggctca ggagcccagg tgggcggacc
     3901 catctcctct ggctcttctg cctccagtgt cacggtcact cgcagctacc gcagtgtggg
     3961 gggcagtggg ggtggcagct tcggggacaa tctggtcacc cgctcctacc tcctgggcaa
     4021 ctccagcccc cgaacccaga gcccccagaa ctgcagcatc atgtaaggat ccgcggccgc
     4081 tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     4141 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     4201 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     4261 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     4321 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     4381 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     4441 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tcacctcaag cgtattcaac
     4501 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     4561 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc ccgaaccacg
     4621 gggacgtggt tttcctttga aaaacacgat gataatatgg agagcgacga gagcggcctg
     4681 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
     4741 gtgggcggcg gagagggcac ccccaagcag ggccgcatga ccaacaagat gaagagcacc
     4801 aaaggcgccc tgaccatcag cccctacctg ctgagccacg tgatgggcta cggcttctac
     4861 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
     4921 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
     4981 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtggtggg caccggcttc
     5041 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
     5101 ctgcacccca tgggcgataa cgtgctggtg ggcagcttcg cccgcacctt cagcctgcgc
     5161 gacggcggct actacagctt cgtggtggac agccacatgc acttcaagag cgccatccac
     5221 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggagctgcac
     5281 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc catcgccttc
     5341 gccagatccc gcgctcagtc gtccaattct gccgtggacg gcaccgccgg acccggctcc
     5401 accggatctc gctaagatat cggccgcgaa ggatctgcga tcgctccggt gcccgtcagt
     5461 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     5521 cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     5581 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     5641 tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc
     5701 cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc
     5761 cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca
     5821 ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct
     5881 ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc
     5941 gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg gagagcgacg
     6001 agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg aacggcgtgg
     6061 agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg accaacaaga
     6121 tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac gtgatgggct
     6181 acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc ctgcacgcca
     6241 tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc ggcgtgctgc
     6301 acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg
     6361 gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc agcaacgcca
     6421 ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc gcccgcacct
     6481 tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg cacttcaaga
     6541 gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg
     6601 aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc ttcaagaccc
     6661 ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg
     6721 gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc ggtgacgtgg
     6781 aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6841 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6901 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6961 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     7021 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     7081 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     7141 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7201 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7261 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7321 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7381 gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7441 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7501 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7561 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7621 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     7681 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     7741 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     7801 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7861 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7921 ggcctcttcc gcgtctccgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7981 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8041 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     8101 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8161 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8221 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8281 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8341 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     8401 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8461 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8521 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8581 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8641 gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat catgtcatag
     8701 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8761 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8821 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8881 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8941 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9001 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9061 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9841 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9901 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9961 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10021 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10081 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10141 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10201 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10261 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10321 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10381 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10441 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10501 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10561 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10621 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10681 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10741 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10801 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    10861 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
    10921 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    10981 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    11041 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    11101 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
    11161 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    11221 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    11281 cagtcacgac gttgtaaaac gacggccagt gccaagctga cgcgtgtagt cttatgcaat
    11341 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
    11401 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
    11461 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
    11521 tgtatttaag tgcctagctc gatacataaa c
//
