LOCUS       sequence-261539-       12269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a split firefly luciferase reporter based on the full 
            length, human Gal8 and CALCOCO2, which interact following endosomal 
            disruption.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12269)
  AUTHORS   Kilchrist KV, Tierney JW, Duvall CL
  TITLE     Genetically encoded split-luciferase biosensors to measure endosome 
            disruption rapidly in live cells.
  JOURNAL   ACS Sens. 2020 Jun 23. doi: 10.1021/acssensors.0c00103.
  PUBMED    32573202
REFERENCE   2  (bases 1 to 12269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Sens. 
            2020 Jun 23. doi: 10.1021/acssensors.0c00103."
COMMENT     Sequence Label: pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394
FEATURES             Location/Qualifiers
     source          1..12269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1574..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1700..1720
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1740..1951
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     protein_bind    1952..1976
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1977..1986
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4305..4329)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4330..4904,4905..4907,4908..4916)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4497..4514)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4724..4743
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     protein_bind    complement(6849..6869)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6882..7470
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6935..6955)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7353..7364)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7501..8000
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7536..7559)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7933..7952
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        8010..8057
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8076..8474
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8561..8794
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8866..8987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8903..8922)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8957..8976
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9027..9162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9182..9201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9183..9201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9211..9228)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9211..9227)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9220..9242)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9369..9824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9456..9475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9666..9687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9850..9954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9955..10023,10024..10815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10173..10192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10986..11574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11475..11494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11728..11745
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11862..11883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11898..11903,11904..11921,11922..11928)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11936..11952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11941..11963
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11960..11976
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11960..11976
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11995..12015
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11997..12015
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        12043..12269
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcgct agcttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     1621 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     1681 ttcaaaattt tactagtatc aactttgtat agaaaagttg ggctccggtg cccgtcagtg
     1741 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc
     1801 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     1861 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     1921 ttttcgcaac gggtttgccg ccagaacaca ggcaagtttg tacaaaaaag caggctgcca
     1981 ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg
     2041 ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca
     2101 tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga
     2161 gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg
     2221 tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg
     2281 tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca
     2341 tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc
     2401 aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg
     2461 gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg
     2521 acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg
     2581 gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca
     2641 gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg
     2701 tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct
     2761 ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact
     2821 ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc
     2881 tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca
     2941 gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct
     3001 acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg
     3061 gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta
     3121 agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgtctg
     3181 gtggcggagg ctcgggcgga ggtgggtcgg gtggcggcgg atcaatgatg ttgtccttaa
     3241 acaacctaca gaatatcatc tataacccgg taatcccgtt tgttggcacc attcctgatc
     3301 agctggatcc tggaactttg attgtgatac gtgggcatgt tcctagtgac gcagacagat
     3361 tccaggtgga tctgcagaat ggcagcagca tgaaacctcg agccgatgtg gcctttcatt
     3421 tcaatcctcg tttcaaaagg gccggctgca ttgtttgcaa tactttgata aatgaaaaat
     3481 ggggacggga agagatcacc tatgacacgc ctttcaaaag agaaaagtct tttgagatcg
     3541 tgattatggt gctgaaggac aaattccagg tggctgtaaa tggaaaacat actctgctct
     3601 atggccacag gatcggccca gagaaaatag acactctggg catttatggc aaagtgaata
     3661 ttcactcaat tggttttagc ttcagctcgg acttacaaag tacccaagca tctagtctgg
     3721 aactgacaga gataagtaga gaaaatgttc caaagtctgg cacgccccag cttcctagta
     3781 atagaggagg agacatttct aaaatcgcac ccagaactgt ctacaccaag agcaaagatt
     3841 cgactgtcaa tcacactttg acttgcacca aaataccacc tatgaactat gtgtcaaaga
     3901 ggctgccatt cgctgcaagg ttgaacaccc ccatgggccc tggacgaact gtcgtcgtta
     3961 aaggagaagt gaatgcaaat gccaaaagct ttaatgttga cctactagca ggaaaatcaa
     4021 aggatattgc tctacacttg aacccacgcc tgaatattaa agcatttgta agaaattctt
     4081 ttcttcagga gtcctgggga gaagaagaga gaaatattac ctctttccca tttagtcctg
     4141 ggatgtactt tgagatgata atttattgtg atgttagaga attcaaggtt gcagtaaatg
     4201 gcgtacacag cctggagtac aaacacagat ttaaagagct cagcagtatt gacacgctgg
     4261 aaattaatgg agacatccac ttactggaag taaggagctg gtagacccag ctttcttgta
     4321 caaagtgggc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     4381 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4441 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     4501 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     4561 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     4621 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     4681 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     4741 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4801 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4861 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg
     4921 aggagaccat caaagatccc cccacatcag ctgtcttgct ggatcactgt catttctctc
     4981 aggtcatctt taacagtgtg gagaagttct acatccctgg aggggacgtc acatgtcatt
     5041 ataccttcac ccagcatttc atccctcgtc gaaaggattg gattggcatc tttagagcat
     5101 ttaaatgttt ccaagacaaa ttggaacaag aactactcaa atggaggagc caaggacaga
     5161 aattgcaggt ggggtggaag acaacccgtg agtattacac cttcatgtgg gttactttgc
     5221 ccattgacct aaacaacaaa tcagctaaac agcaggaagt ccaattcaaa gcttactacc
     5281 tgcccaagga tgatgagtat taccagttct gctatgtgga tgaggatggt gtggtccggg
     5341 gagcaagtat tcctttccaa ttccgtccag aaaatgagga agacatcctg gttgttacca
     5401 ctcagggaga ggtggaagag attgagcagc acaacaagga gctttgcaaa gaaaaccagg
     5461 agctgaagga cagctgtatc agcctccaga agcagaactc agacatgcag gctgagctcc
     5521 aaaagaagca ggaggagcta gaaaccctac agagcatcaa taagaagttg gaactgaaag
     5581 tgaaagaaca gaaggactat tgggagacag agctgcttca actgaaagaa caaaaccaga
     5641 agatgtcctc agaaaatgag aagatgggaa tcagagtgga tcagcttcag gcccagctgt
     5701 caactcaaga gaaagaaatg gagaagcttg ttcagggaga tcaagataag acagagcagt
     5761 tagagcagct gaaaaaggaa aatgaccacc tctttctcag tttaactgaa cagaggaagg
     5821 accagaagaa gctcgagcag acagtggagc aaatgaagca gaatgaaact actgcaatga
     5881 agaaacaaca ggaattaatg gatgaaaact ttgacctgtc aaaaagactg agtgagaacg
     5941 aaattatatg taatgctctg cagagacaga aagagagatt ggaaggagaa aatgatcttt
     6001 tgaagaggga gaacagcaga ttgctcagtt acatgggtct ggattttaat tctttgccgt
     6061 atcaagtacc tacttcagat gaaggaggcg caagacaaaa tccaggactt gcctatggaa
     6121 acccatattc tggtatccaa gaaagttctt cccccagccc gctctccatc aagaaatgcc
     6181 ctatctgcaa agcagatgat atttgtgatc acaccttgga gcaacagcag atgcagcccc
     6241 tttgtttcaa ttgtccaatt tgtgacaaga tcttcccagc tacagagaag cagatctttg
     6301 aagaccacgt gttctgccac tctctctctg gtggcggagg ctcgggcgga ggtgggtcgg
     6361 gtggcggcgg atcacccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg
     6421 ctctcatcga caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg
     6481 agcacttctt catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag
     6541 ccccagccga actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg
     6601 ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg
     6661 gtaaaaccat gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca
     6721 agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt
     6781 tggacgcccg caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg
     6841 tgtaatagca actttattat acatagttga tcaattccga taatcaacct ctggattaca
     6901 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     6961 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7021 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7081 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     7141 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     7201 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     7261 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     7321 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     7381 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     7441 gtcggatctc cctttgggcc gcctccccgc atcgggaatt cccgcggttc gaattctacc
     7501 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     7561 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     7621 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     7681 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     7741 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     7801 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     7861 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     7921 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     7981 cctcatctcc gggcctttcg acctcacgtg ttgacaatta atcatcggca tagtatatcg
     8041 gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag
     8101 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     8161 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     8221 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     8281 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     8341 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     8401 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     8461 tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     8521 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8581 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8641 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8701 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8761 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8821 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8881 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8941 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     9001 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9061 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9121 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     9181 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     9241 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9301 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9361 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9421 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9481 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9541 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9601 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9661 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9721 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9781 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     9841 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9901 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9961 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    10021 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    10081 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    10141 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    10201 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10261 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10321 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10381 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10441 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    10501 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    10561 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    10621 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    10681 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    10741 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10801 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    11041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    11101 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    11161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    11221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    11281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    11341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    11401 gaagagagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    11461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    11521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    11581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    11641 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11701 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11761 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    11821 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
    11881 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
    11941 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
    12001 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
    12061 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
    12121 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
    12181 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
    12241 tatttaagtg cctagctcga tacataaac
//
