LOCUS       sequence-26160-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
  TITLE     WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 
            by DDB1-CUL4-ROC1 ligase.
  JOURNAL   Genes Dev. 2008 Apr 1. 22(7):866-71.
  PUBMED    18381890
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Apr 1. 22(7):866-71."
COMMENT     Sequence Label: pcDNA3-FLAG-DDB1
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..100
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacatggact acaaagacga tgacgataaa ccaagcttgg taccgggtat
      121 gtcgtacaac tacgtggtaa cggcccagaa gcccaccgcc gtgaacggct gcgtgaccgg
      181 acactttact tcggccgaag acttaaacct gttgattgcc aaaaacacga gattagagat
      241 ctatgtggtc accgccgagg ggcttcggcc cgtcaaagag gtgggcatgt atgggaagat
      301 tgcggtcatg gagcttttca ggcccaaggg ggagagcaag gacctgctgt ttatcttgac
      361 agcgaagtac aatgcctgca tcctggagta taaacagagt ggcgagagca ttgacatcat
      421 tacgcgagcc catggcaatg tccaggaccg cattggccgc ccctcagaga ccggcattat
      481 tggcatcatt gaccctgagt gccggatgat tggcctgcgt ctctatgatg gccttttcaa
      541 ggttattcca ctagatcgcg ataataaaga actcaaggcc ttcaacatcc gcctggagga
      601 gctgcatgtc attgatgtca agttcctata tggttgccaa gcacctacta tttgctttgt
      661 ctaccaggac cctcaggggc ggcacgtaaa aacctatgag gtgtctctcc gagaaaagga
      721 attcaataag ggcccttgga aacaggaaaa tgtcgaagct gaagcttcca tggtgatcgc
      781 agtcccagag ccctttgggg gggccatcat cattggacag gantcaatca cctatcacaa
      841 tggtgacaaa tacctggcta ttgcccctcc tatcatcaag caaagcacga ttg
//
