LOCUS       sequence-26171-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Furukawa M, Zhang Y, McCarville J, Ohta T, Xiong Y
  TITLE     The CUL1 C-terminal sequence and ROC1 are required for efficient 
            nuclear accumulation, NEDD8 modification, and ubiquitin ligase 
            activity of CUL1.
  JOURNAL   Mol Cell Biol. 2000 Nov . 20(21):8185-97.
  PUBMED    11027288
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2000 Nov . 20(21):8185-97."
COMMENT     Sequence Label: pcDNA3-HA-CUL1 delta C22
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(247..267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        252..581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      432..567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     494..513
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aactcgagga gacgcaaact actgattcag gcggccatcg tgagaatcat gaagatgagg
       61 aaggttctga aacaccagca gttacttggc gaggtcctca ctcagctgtc ctccaggttc
      121 aaacctcgag tccctgtgat caagaaatgc attgactgac taattgagaa agaatatttg
      181 gagcgagtgg atggtgaaaa ggacacctac agttacttgg cttaaccctt ctggatccag
      241 atctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
      301 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
      361 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
      421 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      481 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
      541 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
      601 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
      661 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatnnactgg
      721 gcacaacana naatcngctg ctctgatgcc gccgtgttcc ggctgtcagc gcagggcgcc
      781 nggttctttt tncaaaacga ctgtccgtgc ctgaatgact gcagac
//
