LOCUS       sequence-261788-        4380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC23A3
FEATURES             Location/Qualifiers
     source          1..4380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2505..2604)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2621..2640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2622..2640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2645..2661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2645..2661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2763..2782)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2774..3583
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2841..2860)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3730..4318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4219..4238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgtccc ggtctcccct gaacccttcc cagctgagat ctgtgggcag
      721 ccaggacgca ctggcaccac tgccacctcc agcaccacag aatccctcca cccactcttg
      781 ggatccactg tgcggaagcc tgccatgggg actgtcctgt ctgctggccc tgcagcacgt
      841 gctggtcatg gccagcctgc tgtgcgtgtc ccacctgctg ctgctgtgca gcctgtcccc
      901 aggaggactg tcttacagcc cttcccagct gctggccagc tccttcttta gctgcggcat
      961 gtccaccatc ctgcagacat ggatgggatc caggctgcca ctggtgcagg caccttctct
     1021 ggagttcctg atccctgccc tggtgctgac ctcccagaag ctgcctcggg ccatccagac
     1081 accaggcaac tctagcctga tgctgcacct gtgcagagga ccaagctgtc acggactggg
     1141 acactggaat acatctctgc aggaggtgag cggagcagtg gtggtgtctg gactgctgca
     1201 gggaatgatg ggactgctgg gaagccctgg acacgtgttt ccacactgcg gacctctggt
     1261 gctggcacca agcctggtgg tggcaggact gtccgcccac agggaggtgg cacagttctg
     1321 ctttacccac tggggactgg ccctgctggt catcctgctg atggtggtgt gctcccagca
     1381 cctgggctct tgccagttcc acgtgtgccc ctggcggaga gcctctacat cctctaccca
     1441 cacaccactg cccgtgttta ggctgctgag cgtgctgatc ccagtggcct gcgtgtggat
     1501 cgtgtctgcc ttcgtgggct ttagcgtgat cccacaggag ctgagcgccc caaccaaggc
     1561 accatggatc tggctgcctc acccaggcga gtggaactgg cctctgctga ccccacgcgc
     1621 cctggcagca ggaatctcca tggcactggc agcatctaca agctccctgg gatgctatgc
     1681 actgtgcggc aggctgctgc acctgccacc tccaccccct cacgcatgct ctaggggact
     1741 gagcctggag ggactgggat ctgtgctggc aggcctgctg ggcagcccta tgggcaccgc
     1801 ctctagcttc ccaaacgtgg gcaaggtcgg actgatccag gcaggatccc agcaggtggc
     1861 acacctggtg ggcctgctgt gcgtgggact gggactgagc ccaaggctgg cccagctgct
     1921 gaccacaatc ccactgcctg tggtgggagg cgtgctggga gtgacacagg cagtggtgct
     1981 gtccgccggc ttctcctctt tttacctggc cgacatcgat agcggcagaa atatctttat
     2041 cgtgggcttc tccatcttta tggccctgct gctgccaagg tggttcaggg aggcacccgt
     2101 gctgttttcc accggctggt ctccactgga cgtgctgctg cactccctgc tgacacagcc
     2161 catcttcctg gccggcctgt ctggctttct gctggagaac accatccctg gaacacagct
     2221 ggagagggga ctgggacagg gactgccaag ccccttcacc gcacaggagg caaggatgcc
     2281 tcagaagcca cgcgagaagg ccgcccaggt gtatcgcctg ccttttccaa tccagaatct
     2341 gtgcccctgt atcccccagc ctctgcactg cctgtgccca ctgccagagg accctggcga
     2401 tgaggaggga ggaagctccg agccagagga gatggcagat ctgctgcctg gaagcggaga
     2461 gccttgccca gagtctagca gggagggctt cagatcccag aaggacccag ctttcttgta
     2521 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2581 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2641 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2701 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2761 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2821 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2881 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2941 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3001 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3061 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3121 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3181 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3241 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3301 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3361 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3421 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3481 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3541 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3601 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3661 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3721 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3781 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3841 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3901 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3961 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4021 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4081 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4141 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4201 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4261 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4321 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
