LOCUS       sequence-26183-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Zacharek SJ, Xiong Y, Shumway SD
  TITLE     Negative regulation of TSC1-TSC2 by mammalian D-type cyclins.
  JOURNAL   Cancer Res. 2005 Dec 15. 65(24):11354-60.
  PUBMED    16357142
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2005 Dec 15. 65(24):11354-60."
COMMENT     Sequence Label: pcDNA3-HA-APC11
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             114..140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             120..146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(464..482)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(465..482)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(502..519)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    508..732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct atgtacccat acgatgttcc tgactatgcg ggctatccct
      121 atgacgtccc ggactatgca agcttggtac cgttaacatg gatccccatg aaggtgaaga
      181 ttaagtgctg gaacggcgtg gccacttggc tctgggtggc caacgatgag aactgtggca
      241 tctgcaggat ggcatttaac ggatgctgcc ctgactgcaa ggtgcccggc gacgactgcc
      301 cgctggtgtg gggccagtgc tcccactgct tccacatgca ttgcatcctc aagtggctgc
      361 acgcacagca ggtgcagcag cactgcccca tgtgccgcca ggaatggaag ttcaaggagt
      421 gaggcccgac ctggctctcg agcatgcatc tagagggccc tattctatag tgtcacctaa
      481 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      541 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
      601 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
      661 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
      721 tgggctctat ggcttctgan gcggaaagaa ccagctgggg ctctaggggg tatccccacg
      781 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      841 cacttgccag cgccctagcg cccgctcctt t
//
