LOCUS       sequence-261921-        4827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC26A6
FEATURES             Location/Qualifiers
     source          1..4827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2952..3051)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3068..3087)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3069..3087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3092..3108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3092..3108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3210..3229)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3221..4030
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3288..3307)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4177..4765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4666..4685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggac tggcagacgc aagcggacca agggatacac aggccctgct
      721 gtccgccacc caggcaatgg acctgcggag aagggattac cacatggagc gcccactgct
      781 gaatcaggag cacctggagg agctgggccg gtggggatcc gccccacgca cacaccagtg
      841 gcggacctgg ctgcagtgct ctagggcaag ggcatacgca ctgctgctgc agcacctgcc
      901 cgtgctggtg tggctgccaa gatatcctgt gagggactgg ctgctgggcg atctgctgtc
      961 cggcctgtct gtggccatca tgcagctgcc tcagggcctg gcatacgcac tgctggcagg
     1021 actgccaccc gtgttcggcc tgtacagctc cttctatcca gtgtttatct atttcctgtt
     1081 tggcacatcc cgccacatct ctgtgggcac ctttgccgtg atgtccgtga tggtgggcag
     1141 cgtgaccgag tccctggcac cacaggccct gaacgactct atgatcaatg agacagcaag
     1201 ggatgcagca agggtgcagg tggcaagcac cctgtccgtg ctggtgggac tgttccaagt
     1261 gggactgggc ctgatccact tcggctttgt ggtgacatac ctgtccgagc ctctggtgcg
     1321 gggatatacc acagcagcag cagtgcaggt gttcgtgagc cagctgaagt acgtgtttgg
     1381 cctgcacctg tctagccact ctggccccct gagcctgatc tataccgtgc tggaggtgtg
     1441 ctggaagctg cctcagtcca aagtgggaac agtggtgacc gcagcagtgg caggagtggt
     1501 gctggtggtg gtgaagctgc tgaatgataa gctgcagcag cagctgccta tgccaatccc
     1561 aggagagctg ctgacactga tcggagcaac cggaatcagc tacggaatgg gcctgaagca
     1621 caggttcgag gtggacgtgg tgggaaacat cccagcagga ctggtgccac cagtggcacc
     1681 caatacacag ctgttttcta agctggtggg aagcgccttc accatcgcag tggtgggctt
     1741 tgccatcgcc atcagcctgg gcaagatctt cgccctgaga cacggctata gggtggactc
     1801 taaccaggag ctggtggccc tgggcctgag caatctgatc ggcggcatct tccagtgctt
     1861 tccagtgtcc tgttctatga gcagatccct ggtgcaggag tccaccggag gaaactctca
     1921 ggtggcagga gccatctcct ctctgttcat cctgctgatc atcgtgaagc tgggcgagct
     1981 gtttcacgat ctgccaaagg ccgtgctggc agcaatcatc atcgtgaacc tgaagggcat
     2041 gctgagacag ctgtccgaca tgaggtctct gtggaaggcc aatagggccg atctgctgat
     2101 ctggctggtg accttcacag ccaccatcct gctgaacctg gacctgggcc tggtggtggc
     2161 cgtgatcttt agcctgctgc tggtggtggt gagaacacag atgcctcact actccgtgct
     2221 gggccaggtg ccagacaccg atatctacag ggatgtggcc gagtattccg aggcaaagga
     2281 ggtgcgcgga gtgaaggtgt tccggagctc cgccacagtg tacttcgcca acgccgagtt
     2341 ttattctgac gccctgaagc agagatgcgg cgtggacgtg gattttctga tcagccagaa
     2401 gaagaagctg ctgaagaagc aggagcagct gaagctgaag cagctgcaga aggaggagaa
     2461 gctgaggaag caggcagcaa gccctaaggg agcatctgtg agcatcaacg tgaataccag
     2521 cctggaggac atgcggtcca acaatgtgga ggattgtaag atgatgcagg tgtctagcgg
     2581 cgacaagatg gaggatgcca cagccaatgg ccaggaggac tctaaggccc cagatggaag
     2641 caccctgaag gccctgggac tgcctcagcc agacttccac tccctgatcc tggatctggg
     2701 cgccctgtct tttgtggaca cagtgtgcct gaagagcctg aagaacatct tccacgactt
     2761 ccgggagatc gaggtggagg tgtatatggc agcatgtcac agcccagtgg tgtcccagct
     2821 ggaggcagga cacttctttg acgcctccat cacaaagaag cacctgttcg cctctgtgca
     2881 cgatgccgtg acctttgccc tgcagcaccc cagacctgtg ccagactctc ctgtgagcgt
     2941 gaccaggctg gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     3001 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     3061 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     3121 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     3181 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     3241 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     3301 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     3361 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     3421 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     3481 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     3541 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     3601 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     3661 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3721 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     3781 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3841 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3901 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3961 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     4021 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     4081 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     4141 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4201 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4261 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4321 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4381 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4441 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4501 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4561 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4621 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4681 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4741 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4801 cttttgctgg ccttttgctc acatgtt
//
