LOCUS       sequence-26211-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Wu S, Ying G, Wu Q, Capecchi MR
  TITLE     A protocol for constructing gene targeting vectors: generating 
            knockout mice for the cadherin family and beyond.
  JOURNAL   Nat Protoc. 2008  . 3(6):1056-76.
  PUBMED    18546598
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2008  . 3(6):1056-76."
COMMENT     Sequence Label: pEYFPpA-ACN
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..52
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    79..128
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(246..265)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ccgaataggg cgaattcata acttcgtata atggtatgct atacgaagtt atggatctgt
       61 cgatcgacgg atcgatccga acaaacgacc caacacccgn tgcgttttat tctgtctttt
      121 tattgccgat cccctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
      181 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
      241 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
      301 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
      361 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
      421 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
      481 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
      541 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
      601 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
      661 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
      721 agccacgata gccgcgctgc ctcgtcctgc agttcattca ggcacggaca ggtcggtctt
      781 ga
//
