LOCUS       sequence-26212-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wu S, Ying G, Wu Q, Capecchi MR
  TITLE     A protocol for constructing gene targeting vectors: generating 
            knockout mice for the cadherin family and beyond.
  JOURNAL   Nat Protoc. 2008  . 3(6):1056-76.
  PUBMED    18546598
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2008  . 3(6):1056-76."
COMMENT     Sequence Label: pEYFPpA-ACN
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(20..53)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             728..739
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             763..783
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcgagctcg cgaaagctta taacttcgta tagcatacat tatacgaagt tatggatctc
       61 catgggccag gcaaatatcc cttaccagcc tcacagagac ctcccccacc ccccgcaacc
      121 ctagagttct tttactagtg agggacaagt ggacaatggt gctgttgtgg gccccaccct
      181 gtgtcccctg tgcccacagt ggtcactctg cttggcaggc aggtgttgca ggctggctgc
      241 tccaggccct ggcaggaggt actgaaggac ctggtaggct cagatgccct ggatgccaag
      301 gcactgctgg agtacttcca accggtcagc cagtggctgg aagagcagaa tcagcggaat
      361 ggcgaagtcc taggctggcc agagaatcag tggcgtccac cgttacccga caactatcca
      421 gagggcattg gtaaagctct gagtgagggt ggactgggac caagagaagt cctggcctct
      481 ggcctctggc ttctgggtca aagcctcagc atcctggtca ctttgctgcc agctgagccc
      541 cagtgtcctt tgcttcagtg ccaagccacc cctgggctca tcctcagggc cctaagcaga
      601 aatgggtatg tctttctctc agggtcctag agacagtgtg cccaagcctg agggcccttg
      661 gggtcaggct ggctggcaca ttgctctatg aggtcacact gcaggcttgg ctcttattgg
      721 ccggtgatgg gagcttcagg gctctgcttt cctgcggcca tgcccaagaa gaagaggaag
      781 gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt
      841 gatgaggttc gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat
      901 acctggaaaa tgcttctgtc
//
