LOCUS       sequence-262256-       19044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Can be used to generate stable Flp-In cells with 
            tetracycline-inducible human polyduction/fibrocystin expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19044)
  AUTHORS   Kaimori JY, Nagasawa Y, Menezes LF, Garcia-Gonzalez MA, Deng J, Imai
            E, Onuchic LF, Guay-Woodford LM, Germino GG
  TITLE     Polyductin undergoes notch-like processing and regulated release 
            from primary cilia.
  JOURNAL   Hum Mol Genet. 2007 Apr 15. 16(8):942-56.
  PUBMED    17470460
REFERENCE   2  (bases 1 to 19044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2007 Apr 15. 16(8):942-56."
COMMENT     Sequence Label: pcDNA5-GFP-PKHD1
FEATURES             Location/Qualifiers
     source          1..19044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(256..275)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     603..624
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(715..732)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    721..945
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1078..1097)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    1229..1276
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1284..2306
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             1284..2306
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    2436..2557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2473..2492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2527..2546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2606..2622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2606..2622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2619..2641)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2630..2646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2654..2660,2661..2678,2679..2684))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2699..2720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2837..2854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3008..3596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3088..3107)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3767..4558,4559..4627))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4390..4409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4628..4732)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4807..4826)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4998..5377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5378..5581
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5532..5552
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    5583..5601
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5604..5622
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5632..5656
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5747..5879
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5862..5881
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5924..5942
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          5973..6545
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      18988..18997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(18994..18996,18997..18999,19000..19044,1..669)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(18994..18996,18997..18999,19000..19044,1..666)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(join(19039..19044,1..16))
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
       61 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      121 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      181 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      241 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      301 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      361 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      421 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
      481 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      541 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      601 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      661 tacaagtaaa gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
      721 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
      781 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
      841 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
      901 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
      961 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     1021 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     1081 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     1141 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     1201 ttgattaggg tgatggttca cgtacctaga agttcctatt ccgaagttcc tattctctag
     1261 aaagtatagg aacttccttg gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     1321 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     1381 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     1441 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     1501 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     1561 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     1621 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     1681 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     1741 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     1801 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     1861 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     1921 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     1981 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     2041 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     2101 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     2161 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     2221 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     2281 gcactcgtcc gagggcaaag gaatagcacg tactacgaga tttcgattcc accgccgcct
     2341 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     2401 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     2461 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2521 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     2581 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2641 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2701 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2761 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2821 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2881 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2941 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3001 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3061 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3121 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3181 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3241 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3301 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3361 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3421 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3481 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3541 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3601 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3661 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3721 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3781 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3841 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3901 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3961 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4021 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4081 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4141 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4201 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4261 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4321 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4381 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4441 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4501 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4561 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4621 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4681 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4741 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     4801 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     4861 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     4921 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     4981 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     5041 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     5101 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     5161 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     5221 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     5281 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     5341 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     5401 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     5461 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     5521 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     5581 tctccctatc agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt
     5641 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     5701 ccgggaccga tccagcctcc ggactctagc gtttaaactt aagcttgtaa gtatcaaggt
     5761 tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt
     5821 gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg
     5881 tgtccactcc cagttcaatt acagctctta aggctagagt acttaatacg actcactata
     5941 ggctagtctg gatcgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt
     6001 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt
     6061 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc
     6121 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt
     6181 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt
     6241 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt
     6301 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc
     6361 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg
     6421 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
     6481 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc
     6541 tacagctcct gggcaacgtg ctggttattg tactgtctca tcattttggc aaagaattga
     6601 tatctgcaga attcgccctt gaaaggatca tttctccctt gagtcacaag gagacagaaa
     6661 cagaaaaaaa agcacaaaag ctatgctgct ccaatcaaaa ctgaaaatgc ttttaatgtc
     6721 tgagcaatct taagagtatt gatttaagtg gacagaatga ctgcctggct gatctctctg
     6781 atgagtattg aagtactact tttggcagta cgtcacctga gtttacatat tgaacctgaa
     6841 gaaggtagcc ttgcaggggg aacgtggatc acagtcattt ttgatggttt ggagttgggt
     6901 gttctttacc ccaacaatgg ctctcaattg gagatacacc tggtgaacgt gaacatggtg
     6961 gtgcccgcat tgcggagtgt tccctgtgac gtctttcctg ttttcttgga tttgcctgtg
     7021 gtgacatgcc ggaccagatc tgtgctgtct gaagcacatg agggtctgta cttcctggaa
     7081 gcatacttcg ggggacagct ggtaagcagt ccaaatccag gaccacgaga tagctgtact
     7141 ttcaagtttt ccaaggcgca gacacccatc gttcaccaag tttatccacc aagtggtgtt
     7201 ccaggaaaac taatacatgt atatggctgg attatcactg gaagattgga aacttttgat
     7261 tttgatgctg agtacattga tagcccagtg atcttggaag ctcaaggaga caaatgggtt
     7321 actccttgct ctcttataaa taggcagatg ggaagctgtt atcctattca ggaggaccat
     7381 ggtcttggga ctctgcagtg ccatgtggaa ggcgactaca tcggctccca gaatgttagc
     7441 ttctcagtat ttaacaaagg aaagtcaatg gtccacaaga aggcatggct gatcagtgct
     7501 aaacaggatc ttttcctata ccagacacac tcagaaatat tatctgtgtt tccagaaact
     7561 gggagccttg ggggaagaac aaacatcaca attacaggag acttttttga caattctgcc
     7621 caggttacca ttgcaggcat tccatgtgat attagacacg tgtctcccag gaagattgag
     7681 tgcaccactc gggctccagg aaaagatgtg aggctcacca cccctcagcc aggcaatcga
     7741 gggcttcttt ttgaagttgg agatgctgtt gagggactgg aactgactga agccacccca
     7801 gggtacaggt ggcagattgt ccctaatgcc agttctccat ttgggttttg gtcacaggaa
     7861 ggacaacctt tcagagcacg gctcagtggg ttctttgtgg ctccagagac aaataattac
     7921 actttctgga ttcaggcaga cagccaagct tccttgcatt tcagttggtc agaggaacca
     7981 aggactaagg tgaaagtggc ctccatcagc gtcggcactg ctgactggtt tgactcctgg
     8041 gagcagaata gggatgaagg gacctggcag cagaagactc ccaagttgga gctgttgggt
     8101 ggagccatgt actacctgga agcagagcat catgggatag ccccaagcag ggggatgagg
     8161 attggtgtcc agattcacaa cacctggctg aatcctgatg tggtcaccac ttacctacgg
     8221 gagaagcacc agatccgagt ccgagcccag aggcttccag aagtacaggt gctgaatgta
     8281 tcaggcagag gaaacttctt ccttacttgg gacaatgtct ctagtcagcc aatccctgca
     8341 aatgccacag cccatctgat tcaaacaacc attgaggagt tacttgcagt aaaatgcaaa
     8401 ctggaacccc tttggtctaa catccttctc cggcttggat ttgaacgagg cccagaagtt
     8461 tccaactctg atggggacct caccagtggg acggagccct tctgtggcag gttcagcctc
     8521 cgtcagcctc gacaccttgt ccttactccc ccggctgccc agaagggcta tcggctagat
     8581 cagtatacac acctgtgtct tgcatacaaa ggccacatga acaagatcct gaagatgatt
     8641 gtgtccttca caatcggctt tcaaaacatg gtaaagaata ccacctgtga ctggagtctc
     8701 acgaggacca gccccgagag ctggcagttc gattgcactg acctctggga gacttgtgtg
     8761 cgttgcttcg gggatctcca gccccctccg gcaaactccc ccgtgctggt tcatcagatc
     8821 aaccttctcc ctctggccca ggagacgggc ctgttctatg tggatgaaat tattattgca
     8881 gacacaaacg taacagtttc tcaagctgat tctggaacgg ctcgcccagg gggcaatctg
     8941 gtggaatcag tctctgtggt gggatcccct ccggtctaca gtgtcacctc ctggctggcg
     9001 gggtgtggca cggagctccc gctcatcact gcacgctctg tgcccactga aggaacagaa
     9061 gagggatctg gactggtcct ggtgacgaca cagagacgac agcggacaag tccacctcta
     9121 ggaggacact ttcgcatcca gcttcctaat acagtgattt ctgatgtccc tgtacaaatt
     9181 tctgctcatc accttcacca gctcttacag aataatgccg atgacttcac atccaggtac
     9241 ctcaatgcca gtgacttcac tgtgaaggag gatctataca cttgctacga acacgtgtgg
     9301 accttgtcct ggtccactca gattggggat ttgcccaatt ttatcagggt ctctgatgaa
     9361 aaccttactg gagtgaatcc tgctgcagcc acgcgtgtgg tatatgatgg tggagttttt
     9421 cttggaccca tatttggaga catgttggct actgccaacc agcatactca ggtggttgtg
     9481 cgagtgaatg atgtaccagc tcattgccca ggttcctgct ctttccagta cctccaaggg
     9541 tcaactccct gtgtccattc tgtgtggtac tccattgatg gtgacatcaa cctaatgatt
     9601 tacattaccg gaactggttt ctctggtgac tcccagttct tgcaggttac agtgaacaaa
     9661 acgagttgca aagttatttt ctcaaaccag accaatgtag tctgtcagac agatttgcta
     9721 cctgttggaa tgcatcggat cttgatgttg gtgagaccct ctggtcttgc catcagtgcc
     9781 actggagaag acctcttcct aaatgtgaaa cctagactgg atatggtgga gccttccaga
     9841 gctgcggata ttggagggct ctgggccacc atccgaggct ctagtttgga aggtgttagc
     9901 ctgatattat ttggatctta ctcgtgtgcc atcaatgtcg ctacaagcaa ttcaagcaga
     9961 attcagtgca aagttccacc cagggggaaa gatggacgca ttgtgaatgt gactgtgatc
    10021 agaggggact attctgcagt tcttcccaga gcatttacat atgtctcttc cttaaatcca
    10081 gttattgtga ctctgagcag aaacataagc aatatagcag gcggtgagac cctggtcatt
    10141 ggagtggcga ggctgatgaa ctatacggat ttggatgtgg aagtccacgt ccaggatgcc
    10201 ttggctccgg ttcacacaca gtcggcttgg ggcctggagg tggcactgcc cccactgcca
    10261 gctggtctcc acagaatttc cgtctctatc aatggggtca gcattcactc acaaggggtt
    10321 gatctccaca tccagtacct cacagaagtt ttcagcatcg agccttgctg tgggtccctg
    10381 ctgggaggga ccatcctcag catctcagga ataggcttca gcagggaccc agctttggtt
    10441 tgggtacttg tgggcaatcg gtcctgtgac attgtgaact taacggaggc gagcatctgg
    10501 tgtgaaaccc tgccagcccc ccagataccc gatgcgggcg ctcccactgt tccagctgcc
    10561 gtggaggtct gggctggcaa caggttcttc gcccgtggtc cttcaccaag cttggtgggg
    10621 aaaggcttca ccttcatgta tgaagcggca gcaacaccag tagtcactgc catgcaagga
    10681 gaaatcacaa atagcagcct gagcctgcat gtgggaggaa gtaacctctc caactcagtc
    10741 atccttctgg ggaacctgaa ctgtgatgtt gagacacagt ccttccaggg caacgtgagc
    10801 ctgtctggat gctccatccc tcttcacagt ctggaggctg gcatctatcc tctccaagta
    10861 cgtcagaagc agatgggatt tgctaatatg tctgtggtgc tccagcaatt tgcagtgatg
    10921 cctcggataa tggccatctt cccatcgcag ggttcggcat gtggtgggac catacttact
    10981 gtgagggggt tgcttcttaa ctctagaagg aggtcagttc gggttgacct ctcgggtcct
    11041 tttacttgtg tgattttgag tttgggagac cacaccattc tctgccaggt tagcctggag
    11101 ggtgacccct tgcctggagc ttccttctcc ctgaacgtca cagccctggt caatgggcta
    11161 accagcgagt gtcaggggaa ttgcactctt ttcataaggg aagaggcaag tcctgtcatg
    11221 gatgccttgt ccacaaacac cagtgggtct ctgaccactg tgctgattag gggtcagagg
    11281 ttagccacca cagctgatga gccgatggta tttgtggatg atcaacttcc ttgcaatgta
    11341 acttttttta atgcaagcca cgttgtgtgc cagacaagag acttggcccc aggaccccac
    11401 tacctgtcag ttttttatac aagaaatggg tatgcttgtt ctggtaatgt ttccagacac
    11461 ttctacatta tgccccaagt gtttcattat tttcctaaga atttcagctt acatggtggg
    11521 agcctcttga ccatagaggg cacaggcctg agaggacaga acaccacgtc agtctatatt
    11581 gaccagcaga cctgcctgac ggtgaacatc ggtgctgagc tcatccggtg cattgttccc
    11641 acagggaatg gctctgttgc cctggaaata gaggtagatg gactttggta tcacatagga
    11701 gtcattggtt ataacaaggc ctttacccca gaattaatct ctatttctca gagcgatgac
    11761 atcttaacct ttgcagtggc ccagatctca ggagctgcaa acattgacat ttttatagga
    11821 atgtcaccct gtgtgggtgt ctctggtaac cacaccgttc ttcagtgcgt ggtcccttcc
    11881 cttccggccg gggagtacca cgtcagaggc tatgactgca tcagagggtg ggcctcatct
    11941 gccctggtgt tcacctcaag agttattatt acagcagtga cggagaactt cggctgcctg
    12001 ggtggaaggc tggtgcatgt gtttggagcg ggattttctc cagggaatgt ctcagctgct
    12061 gtgtgtggtg ctccctgccg agtcctggct aatgctacag tgtctgcctt cagctgcttg
    12121 gttctgcccc tggatgtgtc cttggccttc ctgtgtggcc tgaagcgtga ggaggacagc
    12181 tgtgaggctg ccagacacac ctatgtgcag tgtgatttga cagttgccat ggcgacagag
    12241 caactgcttg aatcgtggcc ttacctctac atttgcgagg aaagttccca atgcctcttt
    12301 gtgccagatc attgggcaga gtcaatgttt ccatcattct cgggcctctt tatcagccct
    12361 aaagtggaaa gagatgaagt tctcatctat aatagctcct gtaacattac catggaaact
    12421 gaggcagaga tggagtgtga gacgcccaat cagccaatta ctgtcaagat tactgagata
    12481 cggaaacgct ggggccagaa cactcagggc aacttttctt tacagttctg ccggagatgg
    12541 tccaggactc acagctggtt tcctgaaagg ctgccacaag atggcgacaa cgtcacagtg
    12601 gagaatggcc aattgcttct gctggacact aacacaagca tcctcaactt actgcacatt
    12661 aaagggggca agctgatttt catggcccca ggacccatcg agctcagggc acacgccatc
    12721 cttgtttctg atggtggaga gctccggatt ggatccgaag acaagccctt ccaaggcaga
    12781 gctcagatca cactctacgg gagttcctac tcaactccct tctttcccta tggagtcaag
    12841 ttcctggctg tgaggaatgg aactctttct ctgcacggtt cactaccaga agtaattgtc
    12901 acctgtctta gagcaactgc ccatgcccta gacacagtgc tggctttaga agatgctgtg
    12961 gactggaacc ctggggatga agttgtcatc atcagtggaa caggtgttaa aggtgccaaa
    13021 ccgatggaag agattgtcac tgtggaaact gtgcaggata cagacctcta tcttaagtca
    13081 cctttgagat attctcacaa ctttacagag aattgggtgg ctggagagca ccatatttta
    13141 aaggccactg tggctctgct cagcaggagt attaccatac aaggaaatct cactaatgag
    13201 agggagaagc tgcttgtttc atgccaggag gccaatgctc cagaaggtaa tctgcagcac
    13261 tgtttgtatt ccatgagtga gaagatgcta ggatccaggg atatgggagc cagagtgatc
    13321 gttcagtcct tcccagaaga gcccagccag gtccagttga agggagtgca gtttcaagtc
    13381 ttggggcaag ccttccataa gcatctgagc tcactcactc tggtgggagc tatgagagag
    13441 tctttcatac agggctgcac agtgaggaac tccttcagta gaggcctcag catgtgcggg
    13501 accttgggcc tgaaggtgga cagtaatgta ttctacaata ttttaggtca tgcgctgcta
    13561 gttgggacat gcacggagat gagatatatc tcctgggagg caattcatgg aaggaaagat
    13621 gactggtcag gacatggaaa tataataaga aacaacgtga tcatccaggt ttctggtgcc
    13681 gagggactct ccaatcctga aatgttgaca ccatctggca tctatatctg cagtcccacc
    13741 aatgttatag aggggaacag agtgtgtggt gctggctatg gctacttttt ccatctcatg
    13801 accaaccaaa catcacaagc tccgcttctt tccttcactc agaacattgc acattcttgt
    13861 accaggtatg gtctctttgt ataccctaaa tttcagccac cttgggataa tgtcactggc
    13921 accactctgt tccagagctt cacagtttgg gaaagtgcag gtggtgccca gatttttaga
    13981 agtagcaatc ttcgcctgaa aaacttcaaa gtttattcat gcagagattt tggaattgac
    14041 gtcttggaaa gtgatgcaaa tacttcagtt actgacagct tattacttgg tcattttgcc
    14101 cacaagggaa gtctgtgtat gtcatctggg attaaaactc ctaaaagatg ggaactgatg
    14161 gtgtctaaca caacctttgt taattttgat ctcatcaact gtgtggccat tagaacctgt
    14221 tcagactgtt cccaaggaca aggtggattt actgtgaaga ccagccagtt gaagtttaca
    14281 aactcttcaa acttagtggc atttccattt cctcatgcag caattttgga agacttggat
    14341 ggatctctgt ctgggaaaaa cagaagtcac attcttgctt ctatggaaac cctttcagct
    14401 tcttgtttgg tcaattcaag ctttggtcgg gttgtccatg gcagtgcctg tggaggaggt
    14461 gttctttttc atcgtatgtc tattggttta gcgaatactc ctgaagtttc ttatgattta
    14521 accatgactg acagcagaaa taaaacaacc actgtcaatt atgtacgtga tacattgtct
    14581 aaccctcgtg gctggatggc tctgctcttg gaccaagaga cctactcatt gcaatctgag
    14641 aacctttgga tcaacagatc tctgcagtac tcagcaacct ttgacaactt tgctcctggt
    14701 aattacctac tgctggtgca cacagatttg ccgccttacc ctgacatcct cctaagatgt
    14761 gggagtcgag tgggtctgtc ttttccattt cttccatcac caggtcagaa ccaaggctgt
    14821 gactggttct tcaatagcca gctgaggcaa ctcacctatc tggtttcagg tgaaggccaa
    14881 gttcaagtca ttctccgggt gaaggaaggt atgcccccaa ctatttcagc ttctacctct
    14941 gcccctgaat cagctttaaa atggtccctc cctgaaacat ggcaaggtgt tgaagaaggc
    15001 tggggaggat acaacaatac cattccaggc cctggggatg acgttctcat tttacccaac
    15061 agaactgtcc ttgtggatac agatcttcca ttcttcaaag ggctgtatgt gatggggacc
    15121 ttagacttcc ctgtggacag aagcaatgtt ctgagtgtgg catgcatggt cattgcaggc
    15181 ggggagctga aagttggtac tttagaaaat cccttagaaa aggaacaaaa gcttctgatt
    15241 ctccttagag cctcagaggg agtcttttgt gaccgtatga atggaattca tattgaccca
    15301 ggaacaattg gggtttatgg gaaagttcat ctttacagtg cttatcctaa gaactcctgg
    15361 acacatcttg gagctgatat tgcctcagga aatgagagaa ttatagtaga agatgcagtg
    15421 gattggcgcc cccatgacaa aatagtcctt agctcctctt cttatgagcc tcatgaagca
    15481 gaggtcctca ctgtgaaaga agtcaagggc caccatgtga ggatctatga acggctcaaa
    15541 caccggcata ttggaagtgt acatgtcacg gaggatggcc gacacattcg tttggctgct
    15601 gaggttggac tgttgacccg aaatatacaa attcagcctg acgtatcatg tagggggaga
    15661 ctgtttgtgg ggtccttcag gaagtccagc cgagaagaat tttcaggtgt ccttcaactt
    15721 cttaatgtgg aaattcagaa cttcgggtca ccattgtact catctgttga attcagtaat
    15781 gtgtcagcag gatcctggat catatcatct actctgcacc agagctgtgg cgggggcatt
    15841 catgcagctg ccagtcatgg agtactttta aatgacaata ttgtgtttgg cacagctggc
    15901 catggcatag atttagaggg tcaggcctat actgtcacta ataaccttgt ggttctgatg
    15961 acacagccag cgtggtccac catttgggtg gcaggaatca aagtgaacca ggtaaaggac
    16021 atcaacctcc atggcaacgt tgtggcagga tcagagagac ttggctttca catccgaggc
    16081 cacaagtgct cctcttgtga actgctttgg tctgacaatg tggcgcattc aagtcttcat
    16141 ggccttcatc tctataagga aagtggactt gacaactgta ccagaatctc tggcttcttg
    16201 gctttcaaga actttgacta tggtgccatg ttacatgtag agaacagcgt ggagatagag
    16261 aacattactc tggtagacaa tactattggt cttttggcag tagtgtatgt attttctgct
    16321 ccacaaaatt ccgtcaaaaa agtgcagatt gtgcttagga attcagtcat tgtggccacc
    16381 agctcttctt ttgactgcat tcaggacaaa gtgaagccgc actcagccaa cttgacatca
    16441 acagatagag ctccctccaa tccaagagga ggtcgaattg gtattctgtg gcctgtattc
    16501 acctcagaac caaatcagtg gcctcaggag ccatggcaca aagtgaggaa tgatcattca
    16561 atttcaggaa tcatgaaact tcaagatgtt accttttcta gttttgtgaa gagttgctat
    16621 agcgatgacc tggatgtctg cattctacca aatgcagaga acagtggaat tatgcaccca
    16681 ataacagcag agaggaccag gatgctaaag ataaaagata aaaacaagtt ctactttcct
    16741 tcattacaac ccaggaaaga tttaggaaaa gtagtctgtc ctgaattaga ctgtgcaagt
    16801 ccaagaaaat atctcttcaa ggatctggat gggagagccc tgggtctgcc tccaccagtt
    16861 tctgtatttc ctaaaacaga ggcagaatgg actgcatcct tcttcaacgc aggtacattt
    16921 agagaagaac agaaatgtac ataccaattt ctgatgcaag gattcatctg caaacagact
    16981 gaccaagtgg tcctaattct tgatagcgct gatgccattt gggcaattca gaagttatat
    17041 ccagttgtat ctgtgactag tggttttgtt gatgtcttta gcagtgtaaa tgccaatatt
    17101 ccctgctcta cttctgggtc agtgtctact ttctattcta tcttacccat caggcaaatc
    17161 accaaagtct gcttcatgga tcaaactcct caagttttgc gcttttttct attggggaac
    17221 aaaagtacct ccaagcttct cttggctgta ttctaccatg agctccagag cccccacgtc
    17281 ttcttagggg aaagttttat tccacccact ctggttcagt cagcttcctt attgctgaat
    17341 gaatctattg gtgccaacta tttcaacatc atggataacc tcttgtatgt tgtcctacaa
    17401 ggagaggagc ccattgaaat acgctcaggt gtttccattc acttggccct cactgtgatg
    17461 gtttcagtct tagaaaaagg ctgggaaata gtaatactcg aaagactaac taacttctta
    17521 cagattggcc aaaaccaaat caggtttatt cacgagatgc ctggccatga agagacctta
    17581 aaggccattg ctgacagtag agcaaaaaga aagcgcaatt gccctactgt gacttgcact
    17641 agtcattata gaagagttgg tcaacgtagg cctctcatga tggaaatgaa ctcacatagg
    17701 gcttcacccc caatgactgt ggaaactatc tcaaaagtga ttgtcattga aattggtgat
    17761 tcgccaacag taaggagcac tggaatgatt tcatccttat caagtaacaa attacagaat
    17821 ttggctcatc gagtcatcac tgctcaacag actggggtac tagagaatgt tctgaatatg
    17881 actatcgggg ccttactagt tactcagtca aagggagtca ttggctatgg aaatacaagc
    17941 agttttaaaa ctgggaactt gatatatatt cggccctatg cactttccat cctagtccag
    18001 ccttcagatg gagaagtggg aaatgagctt ccagtgcagc cacaattggt atttttggat
    18061 gagcagaatc gaagagtaga gtccctggga cctccttcag agccatggac aatttcagct
    18121 tccctggaag gagcatcaga ctcagtgcta aaagggtgca cccaggcaga aactcaagat
    18181 ggttatgtta gcttctacaa cttggcagtc ttgatctctg ggtcaaactg gcactttatt
    18241 tttactgtca cttctcctcc aggagtcaat tttacagctc gatccaagcc atttgctgtc
    18301 ttgcctgtga ctaggaagga gaagtcgacc atcatcctgg ctgctcccct gtcctctgtg
    18361 gcctcatggc tggctctgag ctgtctggtg tgctgttggc ttaaaagaag caaaagcaga
    18421 aaaacaaaac ctgaagagat tcctgaatcc cggactaata atcaaaatat tcatatccac
    18481 atctcatcca aacgccgaga atcacaaggg cccaaaaaag aagacactgt ggtgggagaa
    18541 gatatgagaa tgaaggtcat gctgggcaag gtgaaccagt gcccccacca gttgatgaat
    18601 ggagtgtcca gaaggaaagt tagccgccac attgtccgag aggaagaggc tgctgtgcct
    18661 gctcctggta ctactggcat cacatcccat gggcacatct gtgctccagg tgctcctgct
    18721 cagcaggtgt acctgcaaga gactgggaac tggaaggagg gccaagagca gttgctcaga
    18781 taccagctgg caggccaaaa tcagctgctg ctgctatgcc cagacttcag acaagagagg
    18841 cagcagttgc cagggcaaag tcggctgagt aagcaaagtg gcagcttggg gctttcccaa
    18901 gagaagaaag cctcctgcgg ggccactgag gcattctgcc ttcattcagt acacccggaa
    18961 actattcagg agcaactggc accggtcgcc accatggtga gcaagggcga ggagctgttc
    19021 accggggtgg tgcccatcct ggtc
//
