LOCUS       sequence-262525-        1414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple 
            cloning sites to swap genes-of-interest (i.e. TNFSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF92
FEATURES             Location/Qualifiers
     source          1..1414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..777
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(215..233)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     483..502
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             784..801
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             835..855
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtacagca tgcagctcgc atcctgtctt gcactgacgc tcgcactcct tgtcagcagt
       61 gcacccgtcg acgtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      121 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      181 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      241 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      301 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      361 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      421 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      481 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      541 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
      601 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
      661 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
      721 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggct
      781 agccatcatc accatcacca ttccgctggc tccgctgctg gttctggcga attcgagaac
      841 ctttacttcc agggacccgg gagggcaaat cttgcccacc ctcacgccgc cgaagggctc
      901 tctgcccccc agtttcctgc tcagagcccc tatgcccagc tggtgatgaa agatgccttg
      961 gtgcagaacc agacactgag ctggcacaat gatcctcatc tttctcgtgt gttcctggac
     1021 ccacgaatga attacgatgc tgataaagga gagctggagg tcaaagtcgc cgggctttac
     1081 ttcgtctaca ctcacctgaa gctgcagcag gttgtggttc ctgcatacag tcaaggtaca
     1141 gtcgaggtct ttgtgactgt ggtgcaggac tttggacagc gagctgtctt gaatctctcc
     1201 ctggatgtgg gaccttccag cacttccacc atcaccacct cggagtcctc cctcatcttc
     1261 ctgcatcctg gggagcggct caggctcagc atgagcgcca cagcagggga cttcctggac
     1321 tggcagctgg tgtcaggggc caataccttt ggccttttct gggtggccgg gggacagccc
     1381 ttaaccgatt ctgggacatt aactgtgatc aatt
//
