LOCUS       sequence-262659-       10431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter - constitutive expression of mVenus 
            (515/528) localized in the nucleus, mCherry (587/610) untargeted and
            visualized in the cytoplasm, and Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Calderon GA, Thai P, Hsu CW, Grigoryan B, Gibson SM, Dickinson ME, 
            Miller JS
  TITLE     Tubulogenesis of co-cultured human iPS-derived endothelial cells and
            human mesenchymal stem cells in fibrin and gelatin methacrylate 
            gels.
  JOURNAL   Biomater Sci. 2017 Jun 29. doi: 10.1039/c7bm00223h.
  PUBMED    28661522
REFERENCE   2  (bases 1 to 10431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biomater 
            Sci. 2017 Jun 29. doi: 10.1039/c7bm00223h."
COMMENT     Sequence Label: hEF1-alpha-H2B-mVenus-IRES-mCherry PGK-Puromycin
FEATURES             Location/Qualifiers
     source          1..10431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          116..1038
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     995..1015
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(1714..1725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    2252..2803
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2396..2413)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2623..2642
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    4379..4967
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4432..4452)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4850..4861)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5060..5293
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        5330..5421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5422..6231
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5489..5508)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6317..6905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6806..6825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7043..7269
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7270..7450
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7497..7622
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8115..8348
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8533..8577
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8844..8961
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    9006..9054
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     9079..9098
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(9156..9755)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9239..9259)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9736..9755
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(9767..10266)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9815..9834)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     10208..10231
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     promoter        join(10309..10431,1..1047)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
       61 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
      121 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
      181 aattacttcc acctggctcc agtacgtgat tcttgatccc gagctggagc caggggcggg
      241 ccttgcgctt taggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
      301 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
      361 ctagccattt aaaatttttg atgacgtgct gcgacgcttt ttttctggca agatagtctt
      421 gtaaatgcgg gccaggatct gcacactggt atttcggttt ttgggcccgc ggccggcgac
      481 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
      541 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
      601 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
      661 gaaagatggc cgcttcccgg ccctgctcca gggggctcaa aatggaggac gcggcgctcg
      721 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
      781 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctggagc
      841 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
      901 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
      961 tttggccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     1021 ttttttcttc catttcaggt gtcgtgaaca cctgcgaacg gggtgagacg acacccaagc
     1081 tgtctagagc cgccaccatg cccgagcctg cgaagtcagc cccagcacct aagaagggaa
     1141 gtaagaaggc cgtcaccaaa gcgcagaaga agggagggaa gaagagaaag cggagccgga
     1201 aggaaagcta ctccatctac gtgtacaaag tgctgaaaca agtgcatccg gacactggta
     1261 tcagctccaa ggccatgggc atcatgaact cctttgtgaa cgatatcttc gagcgcattg
     1321 ccggagaggc ttcccgcctg gcccactaca acaagcgcag caccataacc agccgcgaaa
     1381 ttcagaccgc cgtgcgactc ctgctgcccg gagaactggc caagcacgcc gtgtccgagg
     1441 gcactaaggc cgtgaccaag tacacttcag caaaggaccc cgtctgcgag cacccgggaa
     1501 tctgtgggtc gaccatggtg tcgaagggag aggagctgtt caccggggtg gtgccgattc
     1561 tggtggaact tgatggcgac gtcaatggac acaagttctc agtgtccggg gagggagaag
     1621 gagatgctac ctatgggaag ctgactctga agctcatctg cacaacggga aagctgccgg
     1681 tgccgtggcc tactctggtc accaccttgg gttacggcct tcaatgcttc gcgcggtacc
     1741 cagaccacat gaagcaacat gacttcttta agtccgccat gcccgaagga tacgtgcagg
     1801 aaagaactat cttcttcaag gatgacggaa actacaagac cagggctgaa gtgaaattcg
     1861 agggagacac tctcgtgaac cggatcgaac tgaaggggat cgactttaag gaagatggca
     1921 acattctggg gcacaagctg gaatacaatt acaactcgca taacgtctac atcaccgcgg
     1981 acaagcagaa gaacgggatc aaggccaact tcaagattag gcacaacatc gaggacggcg
     2041 gagtgcagtt ggccgatcac taccagcaga ataccccaat cggagatggc cccgtgttgc
     2101 tccctgacaa ccattacctg tcgtatcagt ccaagctgtc caaggacccc aacgagaagc
     2161 gggaccacat ggtgctcctg gagttcgtga ccgccgccgg catcaccctg ggcatggacg
     2221 agctttacaa atgagaattc ccccccccct aacgttactg gccgaagccg cttggaataa
     2281 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2341 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     2401 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2461 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2521 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2581 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2641 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     2701 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga
     2761 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatgctagc ggccacaacc
     2821 atggtgtcaa agggggagga ggataatatg gcgattatca aggagtttat gaggttcaaa
     2881 gtgcacatgg aaggcagcgt gaacggccac gagttcgaaa tcgaaggaga aggggagggt
     2941 cggccttatg aggggaccca gactgctaag ctcaaggtca ccaagggcgg ccccctgccg
     3001 tttgcctggg acatcctgtc cccccaattc atgtacggtt ccaaggccta cgtgaagcac
     3061 ccagcagata ttcctgacta cctgaagctc tcgttcccgg aaggattcaa atgggagaga
     3121 gtgatgaact tcgaagatgg aggcgtcgtg acagtgaccc aggacagcag cctgcaggac
     3181 ggggagttca tctacaaagt caagttgcgg gggaccaact tcccgtccga cggccccgtg
     3241 atgcaaaaga aaactatggg atgggaggcc tcgtccgaac ggatgtaccc ggaggacgga
     3301 gccctgaagg gagaaatcaa gcagcgcctg aagctgaagg acggaggtca ttacgacgcc
     3361 gaagtcaaga cgacttacaa ggctaagaag cccgtgcagc ttcctggagc gtacaacgtg
     3421 aacatcaagc tggatattac ctcccacaac gaagattaca ccatcgtgga gcagtacgaa
     3481 agagccgagg gacgccattc caccggcgga atggacgaac tctacaaata gggatcccgt
     3541 ctctaaaact gttctcatca catcatatca aggttatata ccatcaatat tgccacagat
     3601 gttacttagc cttttaatat ttctctaatt tagtgtatat gcaatgatag ttctctgatt
     3661 tctgagattg agtttctcat gtgtaatgat tatttagagt ttctctttca tctgttcaaa
     3721 tttttgtcta gttttatttt ttactgattt gtaagacttc tttttataat ctgcatatta
     3781 caattctctt tactggggtg ttgcaaatat tttctgtcat tctatggcct gacttttctt
     3841 aatggttttt taattttaaa aataagtctt aatattcatg caatctaatt aacaatcttt
     3901 tctttgtggt taggactttg agtcataaga aatttttctc tacactgaag tcatgatggc
     3961 atgcttctat attattttct aaaagattta aagttttgcc ttctccattt agacttataa
     4021 ttcactggaa tttttttgtg tgtatggtat gacatatggg ttccctttta ttttttacat
     4081 ataaatatat ttccctgttt ttctaaaaaa gaaaaagatc atcattttcc cattgtaaaa
     4141 tgccatattt ttttcatagg tcacttacat atatcaatgg gtctgtttct gagctctact
     4201 ctattttatc agcctcactg tctatcccca cacatctcat gctttgctct aaatcttgat
     4261 atttagtgga acattctttc ccattttgtt ctacaagaat atttttgtta ttgtctttgg
     4321 gctttctata tacattttga aatgaggttg acaagttagc gtgacgtcaa ttcccgataa
     4381 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4441 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4501 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4561 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4621 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4681 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4741 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4801 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4861 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcctcttcg
     4921 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattgcg
     4981 cacgcagtgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5041 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     5101 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5161 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5221 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5281 aaaatctcta gcatcagaat tggttaattg gttgtaacac tgacccctat ttgtttattt
     5341 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5401 taatattgaa aaaggaagaa tatgagccat attcaacggg aaacgtcgag gccgcgatta
     5461 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     5521 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     5581 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     5641 acggaattta tgccacttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     5701 ttactcacca ctgcgatccc cggaaaaaca gcgttccagg tattagaaga atatcctgat
     5761 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ctcgattcct
     5821 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgcc tcgctcaggc gcaatcacga
     5881 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     5941 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     6001 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     6061 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     6121 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     6181 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta actcatgacc
     6241 aaaatccctt aacgtgagtt acgcgcgcgt cgttccactg agcgtcagac cccgtagaaa
     6301 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6361 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6421 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6481 agttagccca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6541 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6601 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6661 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6721 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6781 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6841 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6901 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6961 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7021 gagctgatac cgctcagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     7081 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     7141 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     7201 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     7261 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7321 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7381 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7441 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     7501 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     7561 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     7621 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     7681 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     7741 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     7801 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     7861 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     7921 aggaacagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     7981 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     8041 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     8101 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     8161 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     8221 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     8281 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     8341 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     8401 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     8461 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     8521 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     8581 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     8641 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     8701 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     8761 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     8821 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
     8881 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     8941 attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgagatccc gaaccccgcg
     9001 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
     9061 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccgg
     9121 tcgagaagct tacgcgtgaa tttcgacgcg gccgctcagg caccgggctt gcgggtcatg
     9181 caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc
     9241 tcgtagaagg ggaggttgcg gggcgcggat gtctccagga aggcgggcac cccggcgcgc
     9301 tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg
     9361 ggcgacacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg
     9421 aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg
     9481 ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc
     9541 gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag
     9601 agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg
     9661 gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg
     9721 gtggcgaggc gcaccgtggg cttgtactcg gtcattgtgg cggcgacgaa aggcccggag
     9781 atgaggaaga ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc
     9841 ctccggagga ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg
     9901 acccacccct tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc
     9961 tgccccaaag gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag
    10021 tgagtcgtgc tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga
    10081 acttcctgac taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg
    10141 gttggcgcct accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc
    10201 aagtgcccag cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc
    10261 ctacccggta gagaaacttg atctgtcgcc gcaattcaag cttcgtgagg ctccggtgcc
    10321 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggctctggc
    10381 tgcaggtaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg a
//
