LOCUS       sequence-262663-        5926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector compatible with ColE1 plasmids with high copy number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Peterson J, Phillips GJ
  TITLE     New pSC101-derivative cloning vectors with elevated copy numbers.
  JOURNAL   Plasmid. 2008 May;59(3):193-201. doi: 10.1016/j.plasmid.2008.01.004.
            Epub 2008 Mar 4.
  PUBMED    18295880
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2008.01.004"; journalName: "Plasmid"; date: 
            "2008-05"; volume: "59"; issue: "3"; pages: "193-201"
COMMENT     Sequence Label: pJPK13
FEATURES             Location/Qualifiers
     source          1..5926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1154..2104)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFRIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2152..2374
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     3519..3536
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3653..3674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3689..3694,3695..3712,3713..3719)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3727..3743
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3732..3754
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3751..3767
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3751..3767
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3763..4338
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFAG
                     FPRQALNRGLPLGFRFSALRHLDPKKLD"
     primer_bind     3786..3806
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3788..3806
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    3819..3926
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     3843..3859
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3843..3859
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3893..3909)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3894..3910)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3934..3953)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3935..3953)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3963..3980)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3963..3979)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3972..3994)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4121..4576
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4208..4227)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4418..4439
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5009..5028)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5020..5835
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             5020..5835
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5093..5112)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa
       61 gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac
      121 tttctggctg gatgatgggg cgattcaggg cagtttgtct gcaagactct atgagaagca
      181 gataagcgat aagtttgctc aacatcttct cgggcataag tcggacacca tgaccaaaat
      241 cccttaacgt gagttttcgt tccactgagc gtcagatctc tgttcggggg ttcactgacg
      301 ctgcgtgact acgatggtca ggttgcggag gctatggccc tggtacgagc gctgaacaaa
      361 atgacgaaag caggtatgcc tgaaagcgtg cgtattgcct gaaaacacaa cccgctacgg
      421 gggagactta cccgaaatct gatttattca acaaagccac tttcagtatg gatttgggtg
      481 ttacgggggg gggttggtgc ttaaaatcgc gctgaggatt tctgatggtg ttaagcgggc
      541 ggttttgaga tgtaaactcg cccgtttaac ataatggatc ttgcgcgcac cgcccgaaca
      601 ccactcgcca caaaaaaccg ccggaacgtc caaaagtacg ggttttgctg cccgcaaacg
      661 ggctgttctg gtgttgctag tttgttatca gaatcgcaga tccggcttca gccggtttgc
      721 cggctgaaag cgctatttct tccagaattg ccatgatttt ttccccacgg gaggcgtcac
      781 tggctcccgt gttgtcggca gctttgattc gataagcagc atcgcctgtt tcaggctgtc
      841 tatgtgtgac tgttgagctg taacaagttg tctcaggtgt tcaatttcat gttctagttg
      901 ctttgtttta ctggtttcac ctgttctatt aggtgttaca tgctgttcat ctgttacatt
      961 gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt
     1021 atctatcttt tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatgta
     1081 acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag
     1141 agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt
     1201 gtttttgcgt gagccatgag aacgaaccat tgagatcata cttactttgc atgtcactca
     1261 aaaattttgc ctcaaaactg gtgagctgaa tttttgctgt taaagcatcg tgtagtgttt
     1321 ttcttagtcc gttatgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat
     1381 tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa
     1441 cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc
     1501 tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact
     1561 catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta atctctatat
     1621 ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag
     1681 agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact
     1741 ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact
     1801 tggcatagtt tgtccactgg aagatccgaa agcctttaac caaaggattc ctgatttcca
     1861 cagttctcgt catcagctct ctggttgctt tagctaatac accataagca ttttccctac
     1921 tgatgttcat catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg
     1981 tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat
     2041 gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag
     2101 acatacatct caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc
     2161 aatgataatt actagtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct
     2221 ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg
     2281 ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga
     2341 taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt
     2401 acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag
     2461 gcttaagtag caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc
     2521 atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg
     2581 gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta
     2641 cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcgg
     2701 agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc
     2761 catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat
     2821 gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca
     2881 atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg
     2941 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg
     3001 agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag
     3061 cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     3121 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     3181 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     3241 gccggcaccc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat
     3301 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     3361 cattcgccat tcaggctgcc tcctaccaag acgacttcaa atccgacccg tagagatctc
     3421 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3481 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3541 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3601 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     3661 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     3721 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     3781 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc
     3841 gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg
     3901 tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3961 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4021 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4081 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4141 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4201 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4261 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4321 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4381 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4441 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4501 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4561 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4621 tatttttcta aatacattca aatatgtatc cgctcatggt gtccgactta tgcccgagaa
     4681 gatgttgagc aaacttatcg cttatctgct tctcatagag tcttgcagac aaactgccct
     4741 gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt
     4801 aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg
     4861 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccacg
     4921 ttgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     4981 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     5041 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     5101 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc
     5161 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     5221 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     5281 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     5341 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     5401 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     5461 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     5521 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     5581 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     5641 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     5701 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     5761 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     5821 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     5881 acttgacggg acggcggctt tgttgaataa atcgaacttt tgctga
//
