LOCUS       sequence-262730-        2317 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of FLCN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Shen K, Rogala KB, Chou HT, Huang RK, Yu Z, Sabatini DM
  TITLE     Cryo-EM Structure of the Human FLCN-FNIP2-Rag-Ragulator Complex.
  JOURNAL   Cell. 2019 Nov 27;179(6):1319-1329.e8. doi: 
            10.1016/j.cell.2019.10.036. Epub 2019 Nov 6.
  PUBMED    31704029
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.10.036"; journalName: "Cell"; date: 
            "2019-11-27- 27"; volume: "179"; issue: "6"; pages: "1319-1329.e8"
COMMENT     Sequence Label: [pSK729] pRK5 HA-FLCN(R164A)
FEATURES             Location/Qualifiers
     source          1..2317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..89
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      1893..1902
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1906..2040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1943..1962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1997..2016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     enhancer        2109..2300
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2132..2152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tcgattgaat tcccgaaccg acagtcggtc tcttcaccaa ggccattcgc gccaccatgg
       61 gctaccccta tgatgtgcct gactacgcag atctcaatgg tggtggtggt gggtcgacga
      121 tgaatgccat cgtggctctc tgccacttct gcgagctcca cggcccccgc actctcttct
      181 gcacggaggt gctgcacgcc ccacttcctc aaggggatgg gaatgaggac agtcccggcc
      241 agggtgagca ggcggaagaa gaggaaggtg gcattcagat gaacagtcgg atgcgtgcgc
      301 acagccccgc agagggggcc agcgtcgagt ccagcagccc ggggcccaaa aagtcggaca
      361 tgtgcgaggg ctgccggtca cttgctgcag ggcacccggg atatatcagc catgataaag
      421 agacctccat taaatacgtc agccaccagc accccagcca cccccagctc ttcagcattg
      481 tccgccaggc ctgtgtccgg agcctgagct gtgaggtctg ccctggccgt gaaggcccca
      541 tcttcttcgg agatgagcag cacggctttg tgttcagcca caccttcttc atcaaggaca
      601 gcctggccgc tggcttccag cgctggtaca gcatcatcac catcatgatg gaccggatct
      661 acctcatcaa ctcctggccc ttcctgctgg ggaaggtccg gggaatcatc gatgagctcc
      721 agggcaaggc gctcaaggtg tttgaggcag agcagtttgg atgcccacag cgtgctcaga
      781 ggatgaacac agccttcacg ccattcctac accagaggaa cggcaacgcc gcccgctcgc
      841 tgacatcgct gacaagtgat gacaacctgt gggcgtgcct gcacacctcc tttgcctggc
      901 tcctgaaggc gtgtggcagc cggctgaccg agaagctcct ggaaggtgct ccgaccgagg
      961 ataccttggt ccagatggag aagctcgctg atttagaaga ggaatcagaa agctgggaca
     1021 actctgaggc tgaagaggag gagaaagccc ctgtgttgcc agagagtaca gaagggcggg
     1081 agctgaccca gggcccggca gagtcctcct ctctctcagg ctgtgggagc tggcagcccc
     1141 ggaagctgcc agtcttcaag tccctccggc acatgaggca ggtcctgggt gccccttctt
     1201 tccgcatgct ggcctggcac gttctcatgg ggaaccaggt gatctggaaa agcagagacg
     1261 tggacctcgt ccagtcagct tttgaagtac ttcggaccat gcttcccgtg ggctgcgtcc
     1321 gcatcatccc atacagcagc cagtacgagg aggcctatcg gtgcaacttc ctggggctca
     1381 gcccgcacgt gcagatcccc ccccacgtgc tctcctcaga gtttgctgtc atcgtggagg
     1441 tccacgcagc cgcacgttcc accctccacc ctgtggggtg tgaggatgac cagtctctca
     1501 gcaagtacga gtttgtggtg accagtggga gccctgtagc tgcagaccga gtgggcccca
     1561 ccatcctgaa taagattgaa gcggctctga ccaaccagaa cctgtctgtg gatgtggtgg
     1621 accagtgcct cgtctgcctc aaggaggagt ggatgaacaa agtgaaggtg ctttttaagt
     1681 tcaccaaggt ggacagtcgc cccaaagagg acacacagaa gctgctgagc atcctgggtg
     1741 cgtccgagga ggacaatgtc aagctgctga agttctggat gactggcctg agcaagacct
     1801 acaagtcaca cctcatgtcc acggtccgca gccccacagc ctcggagtct cggaactgag
     1861 cggccgctaa gtaagtaagg atccccagct tggccgccat ggcccaactt gtttattgca
     1921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     2041 gggaattaat tcggcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta
     2101 ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     2161 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2221 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2281 tagtcagcaa ccatagtccc gcccctaact ccggccc
//
