LOCUS       sequence-263231-        1218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged Golgi marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Schormann W, Hariharan S, Andrews DW
  TITLE     A reference library for assigning protein subcellular localizations 
            by image-based machine learning.
  JOURNAL   J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 
            10.1083/jcb.201904090.
  PUBMED    31968357
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-03-2"; volume: "219(3). pii: 133635. doi"; 
            pages: " 10.1083/jcb.201904090"
COMMENT     Sequence Label: pLVX-EF1a-GalT-IRES-Puromycin
FEATURES             Location/Qualifiers
     source          1..1218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     86..106
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      433..442
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(439..441,442..444,445..903)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLLNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(484..505)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(745..764)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgaaggggga tgagtaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
       61 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
      121 ccatttcagg tgtcgtgagg atctatttcc ggtgaattcc tcgagactag catgaggctt
      181 cgggagccgc tcctgagcgg cagcgccgcg atgccaggcg cgtccctaca gcgggcctgc
      241 cgcctgctcg tggccgtctg cgctctgcac cttggcgtca ccctcgttta ctacctggct
      301 ggccgcgacc tgagccgcct gccccaactg gtcggagtct ccacaccgct gcagggcggc
      361 tcgaacagtg ccgccgccat cgggcagtcc tccggggagc tccggaccgg aggggccaag
      421 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      481 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      541 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      601 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc
      661 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      721 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      781 ttcgagggcg acaccctgct gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      841 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      901 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacac atcgaggacg
      961 gcagcgtgca gctcgcgacc actaccagca acaccccatc gcgacgcccg tgctgctgcc
     1021 gaacactact gagctacagt ccgcctgagc aaagacccac gagagcgcga tcacatgtct
     1081 gctgagttcg tgacgcgcgg atcactctcg gcatgacgac tgacagtaag cggcggattc
     1141 ggccctttcc tcccccctaa cgttttactg cagtcgcatg gaatagcgat gcttggctaa
     1201 aagtaatttc ccccaaag
//
