LOCUS       sequence-263285-        4225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Matsushima K, Morishita K, Yoshimura T, Lavu S, Kobayashi Y, Lew W, 
            Appella E, Kung HF, Leonard EJ, Oppenheim JJ
  TITLE     Molecular cloning of a human monocyte-derived neutrophil chemotactic
            factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and 
            tumor necrosis factor.
  JOURNAL   J Exp Med. 1988 Jun 1. 167(6):1883-93.
  PUBMED    3260265
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Med. 
            1988 Jun 1. 167(6):1883-93."
COMMENT     Sequence Label: pUC19/human IL-8
FEATURES             Location/Qualifiers
     source          1..4225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    1004..1060
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2600..2617)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2600..2616)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2609..2631)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2825..2844)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2944..2966
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3004..3022)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3090..3194
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3195..3263,3264..4055)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLTSRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3413..3432)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca
     1021 ggtcgactct agaggatccc cgggtaccga gctcgaattc actagagcac acaagcttct
     1081 aggacaagag ccaggaagaa accaccggaa ggaaccatct cactgtgtgt aaacatgact
     1141 tccaagctgg ccgtggctct cttggcagcc ttcctgattt ctgcagctct gtgtgaaggt
     1201 gcagttttgc caaggagtgc taaagaactt agatgtcagt gcataaagac atactccaaa
     1261 cctttccacc ccaaatttat caaagaactg agagtgattg agagtggacc acactgcgcc
     1321 aacacagaaa ttattgtaaa gctttctgat ggaagagagc tctgtctgga ccccaaggaa
     1381 aactgggtgc agagggttgt ggagaagttt ttgaagaggg ctgagaattc ataaaaaaat
     1441 tcattctctg tggtatccaa gaatcagtga agatgccagt gaaacttcaa gcaaatctac
     1501 ttcaacactt catgtattgt gtgggtctgt tgtagggttg ccagatgcaa tacaagattc
     1561 ctggttaaat ttgaatttca gtaaacaatg aatagttttt cattgtacca tgaaatatcc
     1621 agaacatact tatatgtaaa gtattattta tttgaatcta caaaaaacaa caaataattt
     1681 ttaaatataa ggattttcct agatattgca cgggagaata tacaaatagc aaaattgagg
     1741 ccaagggcca agagaatatc cgaactttaa tttcaggaat tgaatgggtt tgctagaatg
     1801 tgatatttga agcatcacat aaaaatgatg ggacaataaa ttttgccata aagtcaaatt
     1861 tagctggaaa tcctggattt ttttctgtta aatctggcaa ccctagtctg ctagccagga
     1921 tccacaagtc cttgttccac tgtgccttgg tttctccttt atttctaagt ggaaaaagta
     1981 ttagccacca tcttacctca cagtgatgtt gtgaggacat gtggaagcac tttaagtttt
     2041 ttcatcataa cataaattat tttcaagtgt aacttattaa cctatttatt atttatgtat
     2101 ttatttaagc atcaaatatt tgtgcaagaa tttggaaaaa tagaagatga atcattgatt
     2161 gaatagttat aaagatgtta tagtaaattt attttatttt agatattaaa tgatgtttta
     2221 ttagataaat ttcaatcagg gtttttagat taaacaaaca aacaattggg tacccagtta
     2281 aattttcatt tcagatatac aacaaataat tttttagtat aagtacatta ttgtttatct
     2341 gaaattttaa ttgaactaac aatcctagtt tgatactccc agtcttgtca ttgccagctg
     2401 tgttggtagt gctgtgttga attacggaat aatgagttag aactattaaa acagccaaaa
     2461 ctccacagtc aatattagta atttcttgct ggttgaaact tgtttattat gtacaaatag
     2521 attcttataa tattatttaa atgactgcat ttttaaatac aaggctttat atttttaact
     2581 ttaaaaaaaa ccggaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2641 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2701 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     2761 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2821 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     2881 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     2941 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     3001 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     3061 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3121 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3181 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     3241 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     3301 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     3361 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     3421 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     3481 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     3541 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     3601 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     3661 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     3721 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     3781 tacttactct aacttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     3841 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     3901 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3961 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4021 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     4081 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4141 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4201 ccgtagaaaa gatcaaagga tcttc
//
