LOCUS       sequence-263321-        3900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Taylor SS, Kim C, Vigil D, Haste NM, Yang J, Wu J, Anand GS
  TITLE     Dynamics of signaling by PKA.
  JOURNAL   Biochim Biophys Acta. 2005 Dec 30. 1754(1-2):25-37.
  PUBMED    16214430
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2005 Dec 30. 1754(1-2):25-37."
COMMENT     Sequence Label: pRSETB PKA RIalpha
FEATURES             Location/Qualifiers
     source          1..3900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1301..1319)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1315..1362
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1459..1914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1546..1565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1756..1777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1940..2044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2045..2113,2114..2905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2263..2282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3076..3664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3565..3584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3818..3835
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tggcttccgg caccaccgcc
      121 agcgaggagg agcgcagcct ccgggaatgc gagctctatg tccagaagca caacatccag
      181 gcgctgctca aggactccat cgtgcagctg tgcaccgcac ggcccgagag gcccatggcc
      241 ttcctcaggg aatacttcga gaagctggag aaggaggagg caaagcagat ccagaacctg
      301 cagaaagcag gcagccgtgc agactctcgg gaggatgaaa tctccccgcc gccccctaac
      361 ccggtggtga agggccggcg gcgacgcggg gccatcagcg ctgaggtcta caccgaggag
      421 gatgccgcgt cctatgttcg gaaggttata ccgaaagatt ataagacaat ggctgcttta
      481 gctaaagcca ttgaaaagaa cgtactgttt tcacatcttg atgataacga gagaagtgac
      541 atttttgacg ccatgttccc ggtttccttt attgctggag agactgttat tcagcagggt
      601 gacgaagggg ataacttcta cgtgattgac caaggagaga tggacgtcta tgtcaacaat
      661 gaatgggcaa ccagtgttgg ggaaggaggg agcttcgggg agcttgctct gatttacggg
      721 actcctcgag cggccactgt caaggccaag acgaacgtga aactgtgggg cattgaccgg
      781 gacagctaca gaaggatcct catgggaagc acgctgagaa agcggaagat gtatgaggag
      841 tttcttagta aagtgtctat tttagaatct ctggacaagt gggagcgtct cacggtagct
      901 gatgcattgg aaccagtcca gtttgaagac gggcagaaga ttgtggtaca gggggagccg
      961 ggtgatgagt tcttcattat tttagagggc tcagccgcgg tgctgcagcg gcgctcagag
     1021 aacgaagagt ttgtggaagt gggaaggttg gggccttcag actacttcgg cgagatcgct
     1081 ctgctgatga accggccccg tgcagccacc gtggtggccc gtggcccgct gaagtgcgtc
     1141 aagctggacc ggccgcggtt cgagcgcgtt ctcggcccgt gctccgacat cctcaagcgc
     1201 aacatccagc agtacaacag cttcgtgtcc ctgtctgtct gatgaaagct tgatccggct
     1261 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     1321 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     1381 tccggatctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     1441 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     1501 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     1561 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     1621 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     1681 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     1741 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     1801 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     1861 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     1921 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1981 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2041 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2101 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2161 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2221 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2281 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2341 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2401 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2461 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2521 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2581 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2641 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2701 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2761 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2821 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2881 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2941 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3001 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3061 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3121 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3181 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3241 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3301 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3361 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3421 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3481 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3541 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3601 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3661 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3721 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3781 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3841 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
//
