LOCUS       sequence-263331-       11176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 11176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag PIK3CB
FEATURES             Location/Qualifiers
     source          1..11176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        149..528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        529..732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     683..703
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    953..1152
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1353..1769
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1699..1721
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1737..1753
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1787..1796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1793..1834
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1859..1882
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1889..1913
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             2121..2129
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(3428..3439)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5124..5148)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5207..5668
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5263..5280)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5490..5509
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5673..6467
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5727..5746)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6337..6356
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6654..7123
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        7159..7263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7264..7332,7333..8124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7482..7501)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9890..10478
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10379..10398
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10632..10649
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10753..11176,1..4))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10863..10884)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11075..11094
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgcgccgatc ttcaatattg gccattagcc atattattca ttggttatat agcataaatc
       61 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
      121 ggctcatgtc caatatgacc gccatgttgg cattgattat tgactagtta ttaatagtaa
      181 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      241 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      301 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      361 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      421 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      481 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      541 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      601 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      661 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      721 ataagcagag ctggtttagt gaaccgcgcc agtcctccga ttgactgagt cgcccgggta
      781 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
      841 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
      901 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gcaagctggc
      961 cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc
     1021 gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt
     1081 tctgaacacc cggccgcaac cctgggagac gtcccaggga ctttgggggc cgtttttgtg
     1141 gcccgacctg aggaagggag tcgatgtgga atccgacccc gtcaggatat gtggttctgg
     1201 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttgga
     1261 accgaagccg cgcgtcttgt ctgctgcagc gctgcagcat cgttctgtgt tgtctctgtc
     1321 tgactgtgtt tctgtatttg tctgaaaatt agggccagac tgttaccact cccttaagtt
     1381 tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg gtagatgtca
     1441 agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac gtcggatggc
     1501 cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag gtcttttcac
     1561 ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa gccttggctt
     1621 ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct cctcttcctc
     1681 catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga tcctcccttt
     1741 atccagccct cactccttct ctaggcgccg gccggatcca gaattcgcca ccatggggtc
     1801 ttcaaaatct aaaccaaagg accccagcca gcgccggcgc aggatccgag gttaccttga
     1861 ctacaaagac gatgacgaca agcaattgac aagtttgtac aaaaaagttg gcatgtgctt
     1921 cagtttcata atgcctcctg ctatggcaga catccttgac atctgggcgg tggattcaca
     1981 gatagcatct gatggctcca tacctgtgga tttccttttg cccactggga tttatatcca
     2041 gttggaggta cctcgggaag ctaccatttc ttatattaag cagatgttat ggaagcaagt
     2101 tcacaattac ccaatgttca acctccttat ggatattgac tcctatatgt ttgcatgtgt
     2161 gaatcagact gctgtatatg aggagcttga agatgaaaca cgaagactct gtgatgtcag
     2221 accttttctt ccagttctca aattagtgac aagaagttgt gacccagggg aaaaattaga
     2281 ctcaaaaatt ggagtcctta taggaaaagg tctgcatgaa tttgattcct tgaaggatcc
     2341 tgaagtaaat gaatttcgaa gaaaaatgcg caaattcagc gaggaaaaaa tcctgtcact
     2401 tgtgggattg tcttggatgg actggctaaa acaaacatat ccaccagagc atgaaccatc
     2461 catccctgaa aacttagaag ataaacttta tgggggaaag ctcatcgtag ctgttcattt
     2521 tgaaaactgc caggacgtgt ttagctttca agtgtctcct aatatgaatc ctatcaaagt
     2581 aaatgaattg gcaatccaaa aacgtttgac tattcatggg aaggaagatg aagttagccc
     2641 ctatgattat gtgttgcaag tcagcgggag agtagaatat gtttttggtg atcatccact
     2701 aattcagttc cagtatatcc ggaactgtgt gatgaacaga gccctgcccc attttatact
     2761 tgtggaatgc tgcaagatca agaaaatgta tgaacaagaa atgattgcca tagaggctgc
     2821 cataaatcga aattcatcta atcttcctct tccattacca ccaaagaaaa cacgaattat
     2881 ttctcatgtt tgggaaaata acaacccttt ccaaattgtc ttggttaagg gaaataaact
     2941 taacacagag gaaactgtaa aagttcatgt cagggctggt ctttttcatg gtactgagct
     3001 cctgtgtaaa accatcgtaa gctcagaggt atcagggaaa aatgatcata tttggaatga
     3061 accactggaa tttgatatta atatttgtga cttaccaaga atggctcgat tatgttttgc
     3121 tgtttatgca gttttggata aagtaaaaac gaagaaatca acgaaaacta ttaatccctc
     3181 taaatatcag accatcagga aagctggaaa agtgcattat cctgtagcgt gggtaaatac
     3241 gatggttttt gactttaaag gacaattgag aactggagac ataatattac acagctggtc
     3301 ttcatttcct gatgaactcg aagaaatgtt gaatccaatg ggaactgttc aaacaaatcc
     3361 atatactgaa aatgcaacag ctttgcatgt taaatttcca gagaataaaa aacaacctta
     3421 ttattaccct cccttcgata agattattga aaaggcagct gagattgcaa gcagtgatag
     3481 tgctaatgtg tcaagtcgag gtggaaaaaa gtttcttcct gtattgaaag aaatcttgga
     3541 cagggatccc ttgtctcaac tgtgtgaaaa tgaaatggat cttatttgga ctttgcgaca
     3601 agactgccga gagattttcc cacaatcact gccaaaatta ctgctgtcaa tcaagtggaa
     3661 taaacttgag gatgttgctc agcttcaggc gctgcttcag atttggccta aactgccccc
     3721 ccgggaggcc ctagagcttc tggatttcaa ctatccagac cagtacgttc gagaatatgc
     3781 tgtaggctgc ctgcgacaga tgagtgatga agaactttct caatatcttt tacaactggt
     3841 gcaagtgtta aaatatgagc cttttcttga ttgtgccctc tctagattcc tattagaaag
     3901 agcacttggt aatcggagga tagggcagtt tctattttgg catcttaggt cagaagtgca
     3961 cattcctgct gtctcagtac aatttggtgt catccttgaa gcatactgcc ggggaagtgt
     4021 ggggcacatg aaagtgcttt ctaagcaggt tgaagcactc aataagttaa aaactttaaa
     4081 tagtttaatc aaactgaatg ccgtgaagtt aaacagagcc aaagggaagg aggccatgca
     4141 tacctgttta aaacagagtg cttaccggga agccctctct gacctgcagt cacccctgaa
     4201 cccatgtgtt atcctctcag aactctatgt tgaaaagtgc aaatacatgg attccaaaat
     4261 gaagcctttg tggctggtat acaataacaa ggtatttggt gaggattcag ttggagtgat
     4321 ttttaaaaat ggtgatgatt tacgacagga tatgttgaca ctccaaatgt tgcgcttgat
     4381 ggatttactc tggaaagaag ctggtttgga tcttcggatg ttgccttatg gctgtttagc
     4441 aacaggagat cgctctggcc tcattgaagt tgtgagcacc tctgaaacaa ttgctgacat
     4501 tcagctgaac agtagcaatg tggctgctgc agcagccttc aacaaagatg cccttctgaa
     4561 ctggcttaaa gaatacaact ctggggatga cctggaccga gccattgagg aatttacact
     4621 gtcctgtgct ggctactgtg tagcttctta tgtccttggg attggtgaca gacatagtga
     4681 caacatcatg gtcaaaaaaa ctggccagct cttccacatt gactttggac atattcttgg
     4741 aaatttcaaa tctaagtttg gcattaaaag ggagcgagtg ccttttattc ttacctatga
     4801 tttcatccat gtcattcaac aaggaaaaac aggaaataca gaaaagtttg gccggttccg
     4861 ccagtgttgt gaggatgcat atctgatttt acgacggcat gggaatctct tcatcactct
     4921 ctttgcgctg atgttgactg cagggcttcc tgaactcaca tcagtcaaag atatacagta
     4981 tcttaaggac tctcttgcat tagggaagag tgaagaagaa gcactcaaac agtttaagca
     5041 aaaatttgat gaggcgctca gggaaagctg gactactaaa gtgaactgga tggcccacac
     5101 agttcggaaa gactacagat ctttgccaac tttcttgtac aaagtggttt gatctagtcg
     5161 accaattccg gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5221 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5281 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5341 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5401 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5461 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5521 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5581 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     5641 ggttttcctt tgaaaaacac gatgataata ccatgattga acaagatgga ttgcacgcag
     5701 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5761 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5821 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     5881 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5941 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     6001 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     6061 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     6121 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     6181 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     6241 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     6301 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     6361 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     6421 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     6481 gttcgcatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     6541 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
     6601 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
     6661 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     6721 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     6781 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
     6841 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     6901 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     6961 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     7021 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     7081 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacaggcca gtggggccgc
     7141 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7201 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7261 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7321 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7381 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7441 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7501 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7561 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7621 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7681 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7741 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7801 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7861 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7921 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7981 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8041 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8101 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8161 gatttgcggc cgcgaaaagg acaagcagcg aaaattcacg cccccttggg aggtggcggc
     8221 atatgcaaag gatagcactc ccactctact actgggtatc atatgctgac tgtatatgca
     8281 tgaggatagc atatgctacc cggatacaga ttaggatagc atatactacc cagatataga
     8341 ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc cagatataaa
     8401 ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc cagatataga
     8461 ttaggatagc ctatgctacc cagatataga ttaggatagc atatgctatc cagatatttg
     8521 ggtagtatat gctacccatg gcaacattag cccaccgtgc tctcagcgac ctcgtgaata
     8581 tgaggaccaa caaccctgtg cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag
     8641 atcgcagcaa tcgcgcccct atcttggccc gcccacctac ttatgcaggt attccccggg
     8701 gtgccattag tggttttgtg ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc
     8761 agccaagtta ttacaccctt attttacagt ccaaaaccgc agggcggcgt gtgggggctg
     8821 acgcgtgccc ccactccaca atttcaaaaa aaagagtggc cacttgtctt tgtttatggg
     8881 ccccattggc gtggagcccc gtttaatttt cgggggtgtt agagacaacc agtggagtcc
     8941 gctgctgtcg gcgtccactc tctttcccct tgttacaaat agagtgtaac aacatggttc
     9001 acctgtcttg gtccctgcct gggacacatc ttaataaccc cagtatcata ttgcactagg
     9061 attatgtgtt gcccatagcc ataaattcgt gtgagatgga catccagtct ttacggcttg
     9121 tccccacccc atggatttct attgttaaag atattcagaa tgtttcattc ctacactagt
     9181 atttattgcc caaggggttt gtgagggtta tattggtgtc atagcacaat gccaccactg
     9241 aaccccccgt ccaaatttta ttctgggggc gtcacctgaa accttgtttt cgagcacctc
     9301 acatacacct tactgttcac aactcagcag ttattctatt agctaaacga aggagaatga
     9361 agaagcaggc gaagattcag gagagttcac tgcccgctcc ttgatcttca gccactgccc
     9421 ttgtgactaa aatggttcac taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg
     9481 acagctcatg gggtgggaga tatcgctgtt ccttaggacc cttttactaa ccctaattcg
     9541 atagcatatg cttcccgttg ggtaacatat gctattgaat tagggttagt ctggatagta
     9601 tatactacta cccgggaagc atatgctacc cgtttagggt taacaagggg gccttataaa
     9661 cactattgct aatgccctct tgagggtccg cttatcggta gctacacagg cccctctgat
     9721 tgacgttggt gtagcctccc gtagtcttcc tgggcccctg ggaggtacat gtcggccgca
     9781 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9841 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9901 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9961 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10021 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10081 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10141 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10201 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10261 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10321 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10381 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10441 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10501 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10561 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10621 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10681 ctctccccgc gcgttggccg attcattaat gcaactatgg ttggcgcgtt gtaaacgtta
    10741 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaataga
    10801 ccgaaatcgg caaaatccct tataaatcaa aagaatagcc cgagatagag ttgagtgttg
    10861 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
    10921 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg
    10981 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
    11041 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
    11101 ctaaggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
    11161 atgcgccgct acaggg
//
