LOCUS       sequence-263390-        1894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a donor vector containing N-term 3xFLAG to clone into pSLIK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pEN_TTmiRc2_3xflag_NRF2
FEATURES             Location/Qualifiers
     source          1..1894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..73
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ctagatggac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
       61 tgacgatgac aagactagta tggacttgga gctgccgccg ccgggactcc cgtcccagca
      121 ggacatggat ttgattgaca tactttggag gcaagatata gatcttggag taagtcgaga
      181 agtatttgac ttcagtcagc gacggaaaga gtatgagctg gaaaaacaga aaaaacttga
      241 aaaggaaaga caagaacaac tccaaaagga gcaagagaaa gcctttttcg ctcagttaca
      301 actagatgaa gagacaggtg aatttctccc aattcagcca gcccagcaca tccagtcaga
      361 aaccagtgga tctgccaact actcccaggt tgcccacatt cccaaatcag atgctttgta
      421 ctttgatgac tgcatgcagc ttttggcgca gacattcccg tttgtagatg acaatgaggt
      481 ttcttcggct acgtttcagt cacttgttcc tgatattccc ggtcacatcg agagcccagt
      541 cttcattgct actaatcagg ctcagtcacc tgaaacttct gttgctcagg tagcccctgt
      601 tgatttagac ggtatgcaac aggacattga gcaagtttgg gaggagctat tatccattcc
      661 tgagttacag tgtcttaata ttgaaaatga caagctggtt gagactacca tggttccaag
      721 tccagaagcc aaactgacag aagttgacaa ttatcatttt tactcatcta taccctcaat
      781 ggaaaaagaa gtaggtaact gtagtccaca ttttcttaat gcttttgagg attccttcag
      841 cagcatcctc tccacagaag accccaacca gttgacagtg aactcattaa attcagatgc
      901 cacagtcaac acagattttg gtgatgaatt ttattctgct ttcatagctg agcccagtat
      961 cagcaacagc atgccctcac ctgctacttt aagccattca ctctctgaac ttctaaatgg
     1021 gcccattgat gtttctgatc tatcactttg caaagctttc aaccaaaacc accctgaaag
     1081 cacagcagaa ttcaatgatt ctgactccgg catttcacta aacacaagtc ccagtgtggc
     1141 atcaccagaa cactcagtgg aatcttccag ctatggagac acactacttg gcctcagtga
     1201 ttctgaagtg gaagagctag atagtgcccc tggaagtgtc aaacagaatg gtcctaaaac
     1261 accagtacat tcttctgggg atatggtaca acccttgtca ccatctcagg ggcagagcac
     1321 tcacgtgcat gatgcccaat gtgagaacac accagagaaa gaattgcctg taagtcctgg
     1381 tcatcggaaa accccattca caaaagacaa acattcaagc cgcttggagg ctcatctcac
     1441 aagagatgaa cttagggcaa aagctctcca tatcccattc cctgtagaaa aaatcattaa
     1501 cctccctgtt gttgacttca acgaaatgat gtccaaagag cagttcaatg aagctcaact
     1561 tgcattaatt cgggatatac gtaggagggg taagaataaa gtggctgctc agaattgcag
     1621 aaaaagaaaa ctggaaaata tagtagaact agagcaagat ttagatcatt tgaaagatga
     1681 aaaagaaaaa ttgctcaaag aaaaaggaga aaatgacaaa agccttcacc tactgaaaaa
     1741 acaactcagc accttatatc tcgaagtttt cagcatgcta cgtgatgaag atggaaaacc
     1801 ttattctcct agtgaatact ccctgcagca aacaagagat ggcaatgttt tccttgttcc
     1861 caaaagtaag aagccagatg ttaagaaaaa ctag
//
