LOCUS       sequence-263431-        2982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gata2a endothelial enhancer (x6) and basal promoter in p5E Gateway 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S,
            Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND
  TITLE     Valves Are a Conserved Feature of the Zebrafish Lymphatic System.
  JOURNAL   Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 
            10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26.
  PUBMED    31564611
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.08.019"; journalName: "Dev Cell"; date: 
            "2019-11-4- 4"; volume: "51"; issue: "3"; pages: "374-386.e5"
COMMENT     Sequence Label: p5E-6xgata2aECEbasp
FEATURES             Location/Qualifiers
     source          1..2982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(46..132)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     181..203
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     195..212
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     196..212
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    252..347
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    687..810
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(882..901)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(883..901)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(906..922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(906..922)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1024..1043)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1035..1844
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1102..1121)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1991..2579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2480..2499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2733..2750
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2909..2936)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
       61 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
      121 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
      181 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggcccgag ttaacgctac
      241 catggagctc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
      301 tgataagcaa tgctttttta taatgccaac tttgtataga aaagttgagt tgtttcctat
      361 ccggatatct gcccgggccc gataaagttg tttcctatcc ggatatctgc ccgggcccga
      421 taacacgtca cgcacgtgca gtactagttg tttcctatcc ggatatctgc ccgggcccga
      481 taaagttgtt tcctatccgg atatctgccc gggcccgata acacgtcacg cacgtgcagt
      541 actagttgtt tcctatccgg atatctgccc gggcccgata aagttgtttc ctatccggat
      601 atctgcccgg gcccgataag cttctagagg gtatataatg gatcatcgcg tctcagcctc
      661 actttgagct cctccacacg ctcgagcaag tttgtacaaa aaagttgaac gagaaacgta
      721 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
      781 ctgtaaaaca caacatatgc agtcactatg aaccaactac ttagatggta ttagtgacct
      841 gtagaattcg agctctagag ctgcagggcg gccgcgatat cccctatagt gagtcgtatt
      901 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
      961 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     1021 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     1081 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1141 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1201 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1261 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1321 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     1381 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     1441 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     1501 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     1561 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     1621 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     1681 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     1741 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     1801 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     1861 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     1921 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     1981 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2041 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2101 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2161 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2221 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2281 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2341 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2401 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2461 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2521 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2581 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2641 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2701 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2761 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     2821 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     2881 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     2941 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc ca
//
