LOCUS       sequence-263715-        7681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible lentiviral vector expressing mouse Klf4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Velychko S, Adachi K, Kim KP, Hou Y, MacCarthy CM, Wu G, Scholer HR
  TITLE     Excluding Oct4 from Yamanaka Cocktail Unleashes the Developmental 
            Potential of iPSCs.
  JOURNAL   Cell Stem Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002.
  PUBMED    31708402
REFERENCE   2  (bases 1 to 7681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2019 Oct 30. pii: S1934-5909(19)30423-0. doi: 
            10.1016/j.stem.2019.10.002."
COMMENT     Sequence Label: pHAGE2-TetOminiCMV-Klf4
FEATURES             Location/Qualifiers
     source          1..7681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2251..2269,2270..2292,2293..2311,2312..2334,2335..2353,
                     2354..2376,2377..2395,2396..2418,2419..2437,2438..2460,
                     2461..2479,2480..2502,2503..2521)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2554..2592
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2679..2688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4123..4711
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4176..4196)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4594..4605)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4859..5039
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5086..5104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5172..5276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5277..5345,5346..6137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5495..5514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6308..6896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7050..7067
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7307..7328
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7343..7348,7349..7366,7367..7373)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7381..7397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7386..7408
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7405..7421
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7405..7421
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7471..7490
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat
     2581 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     2641 gacctccata gaagacaccg ggaccgatcc agcctgcggc cgccatggct gtcagcgacg
     2701 ctctgctccc gtccttctcc acgttcgcgt ccggcccggc gggaagggag aagacactgc
     2761 gtccagcagg tgccccgact aaccgttggc gtgaggaact ctctcacatg aagcgacttc
     2821 ccccacttcc cggccgcccc tacgacctgg cggcgacggt ggccacagac ctggagagtg
     2881 gcggagctgg tgcagcttgc agcagtaaca acccggccct cctagcccgg agggagaccg
     2941 aggagttcaa cgacctcctg gacctagact ttatcctttc caactcgcta acccaccagg
     3001 aatcggtggc cgccaccgtg accacctcgg cgtcagcttc atcctcgtct tccccagcga
     3061 gcagcggccc tgccagcgcg ccctccacct gcagcttcag ctatccgatc cgggccgggg
     3121 gtgacccggg cgtggctgcc agcaacacag gtggagggct cctctacagc cgagaatctg
     3181 cgccacctcc cacggccccc ttcaacctgg cggacatcaa tgacgtgagc ccctcgggcg
     3241 gcttcgtggc tgagctcctg cggccggagt tggacccagt atacattccg ccacagcagc
     3301 ctcagccgcc aggtggcggg ctgatgggca agtttgtgct gaaggcgtct ctgaccaccc
     3361 ctggcagcga gtacagcagc ccttcggtca tcagtgttag caaaggaagc ccagacggca
     3421 gccaccccgt ggtagtggcg ccctacagcg gtggcccgcc gcgcatgtgc cccaagatta
     3481 agcaagaggc ggtcccgtcc tgcacggtca gccggtccct agaggcccat ttgagcgctg
     3541 gaccccagct cagcaacggc caccggccca acacacacga cttccccctg gggcggcagc
     3601 tccccaccag gactacccct acactgagtc ccgaggaact gctgaacagc agggactgtc
     3661 accctggcct gcctcttccc ccaggattcc atccccatcc ggggcccaac taccctcctt
     3721 tcctgccaga ccagatgcag tcacaagtcc cctctctcca ttatcaagag ctcatgccac
     3781 cgggttcctg cctgccagag gagcccaagc caaagagggg aagaaggtcg tggccccgga
     3841 aaagaacagc cacccacact tgtgactatg caggctgtgg caaaacctat accaagagtt
     3901 ctcatctcaa ggcacacctg cgaactcaca caggcgagaa accttaccac tgtgactggg
     3961 acggctgtgg gtggaaattc gcccgctcgg atgaactgac caggcactac cgcaaacaca
     4021 cagggcaccg gccctttcag tgccagaagt gcgacagggc cttttccagg tcggaccacc
     4081 ttgccttaca catgaagagg cacttttaaa tcgatagatc ctaatcaacc tctggattac
     4141 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4201 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4261 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4321 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4381 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4441 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4501 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4561 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4621 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4681 agtcggatct ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa
     4741 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     4801 ctcccaacga agacaagatc acctgcagga caggcgcgcc ctgctttttg cttgtactgg
     4861 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4921 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4981 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac
     5041 ccgggcgatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc
     5101 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5161 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5221 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5281 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5341 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5401 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5461 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     5521 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5581 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5641 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5701 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5761 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5821 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5881 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5941 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6001 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6061 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6121 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6181 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6241 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6301 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6361 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6421 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6481 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6541 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6601 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6661 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6721 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6781 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6841 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6901 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6961 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7021 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7081 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca
     7141 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7201 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc
     7261 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7321 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7381 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa
     7441 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7501 atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc
     7561 cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct
     7621 ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
     7681 t
//
