LOCUS       sequence-263730-        9794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nucleo-cytoplasmic mCherry in M-cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, 
            Billault-Chaumartin I, Martin SG
  TITLE     A toolbox of stable integration vectors in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 
            10.1242/jcs.240754.
  PUBMED    31801797
REFERENCE   2  (bases 1 to 9794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-01-8"; volume: "133(1). pii: jcs.240754. doi"; 
            pages: " 10.1242/jcs.240754"
COMMENT     Sequence Label: pAV0762
FEATURES             Location/Qualifiers
     source          1..9794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2697..3152)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2834..2855)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3046..3065
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3278..3300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3292..3309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3293..3309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3319..3338
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3319..3337
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3761..3772
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5134..5838
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(5273..5291)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5541..5560
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      5859..6046
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5914..5938)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            6121..7477
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        6121..6464
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6465..7274
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6532..6551)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      7280..7477
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(7534..7554)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7534..7552)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7573..7589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7573..7589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7586..7608)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7597..7613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7621..7627,7628..7645,7646..7651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7666..7687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7804..7821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7975..8563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8055..8074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8734..9525,9526..9594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9357..9376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9595..9699)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9767..9785
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaccg ggagaatcct gaataatgtg ctgtgaagca gttgaaagaa aattaaagaa
      241 cccaatataa tatgctataa agcaatcaaa aaataatagt aagaaaccca aataaaaccc
      301 cgttgaccat gttcccaatt aaattaacgc atattaatgc aaaaaatgca atattaagac
      361 tagttattaa accgataatt agtcttcaat tattaaaaga tgtagttgat ttgtattatc
      421 tttcaattgc taattctaaa tatattggag aattcaatga gtttatgcat tgaagtttaa
      481 gataacattg gcgctgattg cagcaaaagt aatcacgcat atagctgtag gaccaaaacg
      541 atcatcatat cggaccgcat tatgtttaaa cgttgcttta tatatggata acgacatata
      601 catttatttc ttgaaaaaat agcaaagcta tattgatttt agcgtatgct gctttaaagt
      661 tgcatttcac aatgcttgga aatgtaacga tgacagaatt aatacgatgc aaaactcaaa
      721 taataaactg cgcactaact cactacaata aacaactttg aaaaaaagat tcgttttttc
      781 aacatttacc atctcattaa gctgagctgc caaggtatat acatacttca tcgaatatga
      841 gcgaaaaaca ggttgtaggg atccttggag gtggtcaatt gggccgaatg atggtagagg
      901 cagcccatcg cttaaacatc aaatgcatca tcttggatgc agcaaattct cctgccaaac
      961 aaattgatgg aggacgtgag cacattgatg catcatttac tgaccccgat gcaattgttg
     1021 aactgtctaa gaagtgcacg ttattaacaa ctgaaattga gcatattaac actgatgcct
     1081 tggcagccgt tacgaaatct gttgctgttg aaccctctcc tgcaactctg cgatgcattc
     1141 aagacaaata tcttcaaaaa cagcatttac aggtttttaa gattgcactt cctgaatttt
     1201 gcgatgcacc tgaccaggaa agtgttgaaa aagcaggcca agagtttggt tatccttttg
     1261 tactgaaaag taaaacattg gcttacgacg gtcgtggaaa ttacgttgtt catcaaccat
     1321 ctgagattcc tactgccatc aaagcacttg gtgatcgtcc gctttatgtt gaaaagttcg
     1381 ttcctttctc catggaaatt gcagtgatgg tagtacgcag tttagacgga aaagtttatg
     1441 cttatcctac aactgagacc attcaaaagg ataatgtttg tcatttagta tatgcccctg
     1501 ctcgtcttcc cttctcaatt caacagcgtg ctcaaaccct tgccatggat gcggtgcgca
     1561 cttttgaagg cgctggtata tatggtgtag agatgtttgt tttgcaagat ggtgagacca
     1621 ttttactcaa cgaaattgct cctcggcctc acaattcagg tcactacacc attgaagctt
     1681 gcccaacttc tcagtttgaa gctcacttac gggccatatg tggtcttcct ttcagcgaaa
     1741 tcaacaccca actctcgact tccacaactc atgcgttgat ggtaaatatt ttaggtactg
     1801 atgatcctga ttatgtttca aagatcgcta aacgttctct gtccattccc ggtgcaactt
     1861 tgcatcttta tggtaaagct gaatctagaa agggtcgcaa gatgggacac gttaccatca
     1921 tttctgattc acctcaagaa tgtgaacgta ggtatcagat gcttcttgac gtcaaagatc
     1981 ctgtcgaatc acctgttgtt ggtattatca tgggttcgga ttctgattta agcaagatga
     2041 aagatgctgc cgtcatttta gatgaattca aggtgcctta cgaacttact attgtttcag
     2101 ctcaccgcac accagatcgc atggttactt atgctcgtac cgcagcttca agagggttgc
     2161 gtgtgattat tgctggtgct ggtggtgccg ctcatttgcc tggtatggtt gctgcaatga
     2221 cacctcttcc agtaatcggc gttcctgtaa aaggaagcac tcttgacgga gttgactctc
     2281 ttcactctat tgttcagatg cctcgaggtg tccctgtcgc cactgttgct atcaataata
     2341 gccaaaatgc cggtatttta gcctgtcgta tacttgctac atttcaaccc tcccttttgg
     2401 ctgctatgga gagctttatg gacaatatga aagagattgt tttagaaaag gctgataaat
     2461 tagaaaaagt tggttggaaa aattattctg cataggcgac catagacata actgttaaat
     2521 gattttgctt gctttttgtt ttaaataata ttttattttg aaattctaga ttgtaaaatg
     2581 tattctaaaa agtgattaaa aattaaaaat aaaaaccccc cgaataatgt gctgcgacgc
     2641 agctagggcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     2701 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2761 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2821 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2881 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2941 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     3001 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     3061 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     3121 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     3181 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3301 acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccgct
     3361 agcatgcgag ctttgcgtaa ttatgtacgg cgtcctggga tagtcgctgt ttgttaaaaa
     3421 aaaggtgatt cgcaactgat gtatgcagat ggcgatgaga aagtagagag aggtagtgaa
     3481 aaaagataat gaaatataga gaactacgta acgctccaca atagaacaaa taccgtgcac
     3541 taaagtttca agaaatttcc gcttcaatag gaaagaataa acaaattaga atggtttgtt
     3601 ggtacgggag aaagccgatt acgaacagtc aaggcaatga ttttgattga tatgatcaac
     3661 caattttgat tttactaatt caaaatgaaa tcctattaat ttcaatcgat agctcgcccc
     3721 ttttacttac aattttttga aagatgttgt tgggggatga aaagaggaga aatcgaaagc
     3781 aaacaactgg cacaatatag ccgcgaaaaa aaattttttt aatgtaaata taccgcgtta
     3841 cttgaccttc ctttataagt aaatttttag tcctgtgatc ttccgaggca gcatgtgccg
     3901 tttgcataat ttatgaccaa atccgttacc cacatatatc atttgtttgc cttgagcacc
     3961 ccggataaaa ggttgaagag ttggtttccc gatatatggt ccttacacta aagtaaagga
     4021 tgctcagtaa gcttctcccg tctagactgg aaatagcgca aaaatcattt ggtatgtata
     4081 atcaaatatt acggaacgga gtacgactta aattaattct attaacaaat tgaaagcgca
     4141 tagctctttc agatatgaaa gtgaagcaga aggtgagcat tattcgcact tagcaacaga
     4201 aaatttgtaa ctgacgacct tgcaaaggct tgtaaggtga gctaagttcc gcttccgtct
     4261 acggttctta ccgttcttct cctcgtttcc gtgcgatgcg gtaccgtgta aaggcatacc
     4321 ccgtcttttt ctataactat tttaacgaaa cttgttgaag cttaatcctc acgagttttc
     4381 cgtttgggta tttcgtgtgg tttcggatgt ttcaaaagtt tggggtaatc gacatttgtc
     4441 cgttccccag atttctgaag ataatgttaa ggcttaccga atcaataacc tgacgtgttc
     4501 attcgtacat gtgccattgt tgttgacaaa gaattaaccc catttacaag tatggtgcgg
     4561 acaaccaatt actatatttt taccaaggtg gtcctccaac aaacgaatcc gttcaacgac
     4621 ttgggtcacc ttatataggt tgatttgcaa gaagttttgc ttattccatc tttggacggt
     4681 tgtagcacat aagatgtcca tgttagtacc caaataaagt attttgtttt agtttagata
     4741 attatataaa attaaaacta acttatccaa cttaagcgtg tcagcaatgc aatcaagaac
     4801 tgctattatt tatttacacc ttgacgagta caccacttgt cattcatttt cgtaactttc
     4861 gggagctact ttgaggttgt tcctttcatt cttttttttt tataaattta gttcctttgt
     4921 tatattttat tttattgtaa tctaacttct gcatcttcta tcaaagggat atttcccatt
     4981 gtgctgagta cacaatgggc ctttgttagg tactctccat tgttgtccac catacaaaat
     5041 gaagggcttg tcctctcttc agacaccatt cggtgcggtt tctttggcaa agaatcactc
     5101 tttggatttt gttttttaga attcatggca aggagcaagg gcgaggagga taacatggcc
     5161 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     5221 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     5281 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     5341 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     5401 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     5461 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     5521 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     5581 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     5641 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     5701 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     5761 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     5821 gacgagctgt acaagtaagg cgcgccactt ctaaataagc gaatttctta tgatttatga
     5881 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     5941 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     6001 ctcaggtata gtatgaggtc gctcttattg accacacctc taccggcaga tccgctaggg
     6061 ataacagggt aatatagatc tgtttagctt gcctcgtccc cgccgggtca cccggccagc
     6121 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
     6181 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
     6241 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
     6301 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
     6361 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
     6421 ttgctaggat acagttctca catcacatcc gaacataaac aaccatgggt aaggaaaaga
     6481 ctcacgtttc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     6541 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg
     6601 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     6661 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     6721 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggcaaa acagcattcc
     6781 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     6841 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     6901 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     6961 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag cttttgccat
     7021 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     7081 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     7141 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     7201 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     7261 atgagttttt ctaatcagta ctgacaataa aaagattctt gttttcaaga acttgtcatt
     7321 tgtatagttt ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat
     7381 tttttttcgc ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc
     7441 atgcgtcaat cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg
     7501 ccatccagtt taaacgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     7561 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7621 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7681 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     8041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     8101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     8161 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8401 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8641 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8701 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8761 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8821 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8881 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8941 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9001 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     9061 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9121 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9181 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9241 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9301 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9361 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9421 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9481 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9541 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9601 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9661 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9721 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     9781 gaggcccttt cgtc
//
